LOCUS       sequence-317038-        4292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobius Assembly cloning and expression vector for plants with 
            high-copy number origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pMAP L1B
FEATURES             Location/Qualifiers
     source          1..4292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      50..638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     539..558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2160..2184
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    2211..2235
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2288..2322
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2331..2342
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3031..3102
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3118..3145
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3375..4190)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4182..4201
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 atcggattgg cggttatgcg gttcccgtag aaaggatcaa aggatcttct tgagatcctt
       61 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      721 attctgtgga taaccgtatt accgcctttg agtgagctgc cgatccgccg ccagttcagg
      781 ccgtccggcc ccggattctg accataattt catcgaaaaa aggggcgcag cccttcttgt
      841 tctaatagtt ctataagttc aggcgaaaat cgtgcagcaa ttacaaaagg ttgcgcgtct
      901 ataagtgggg aatccagacg caaaagtggg gaatccagcc gcaaaagtgg ggaatccagc
      961 cgaaatcgcg gattgacgag tgggatttcc cgcccaataa atccaccatg ggaaagacac
     1021 tcgacgttgc ccgcgaccgg gcctttgacc agaccgcgac cgtgctgccc gccgaaatgg
     1081 cgcggggggt ctatatgcgc aacgccccca gcctcgcggc cctgaagctg atgcatctga
     1141 tgatcgccac ggcgggcggg cgcatggccg atgacgtgcg ccacgaaatg cggctggccg
     1201 acatccgcaa gatcgacggt atggataacc acacccgggc cagcctgacc ccgctctttg
     1261 cggaactggc ggcggcggtg ctgacccacg acgacccgga aaagcgggtc gtgaccatcg
     1321 gtggcctgct ggacgaagcc cggatcgatt accggcacga ggtcagcggc gatcttctgg
     1381 tgtcgtggac cttccgcagc atgttccgcc gcatggcggc ggaatcgaac cattgggcga
     1441 ttctcgaccg gcagaccgtg ttccacctcg gcagcaagta ttccgtgctg ttgttccagc
     1501 acatcgccag cttcaaggaa tacgaccaca ttaccggcaa gacctttacc gtgccggagt
     1561 tgcgggctgt gtttggtatc cccgagggca aaatcaagcg tttcgcagac ctcaacagag
     1621 acgtgctgac gcccgccatt gccgaaatca accagctttc ccgcctgact ctgaccgcca
     1681 cgccgaacaa gatcgggcgc accgtggcca gcgtgacgat tgcttgggaa gaaaagcccc
     1741 tcgaaggcaa gcgctcgacc aaggccgaac tggaccgccc gaaggtgggc cggaaggccc
     1801 ggcgcgacgg caccgccgag acggtggcac gggccttccc ggcatcgggc gggatcgagt
     1861 tcgaccagca ttggcgcgac ctcaagcggg cggcgggctg caacatggac aacaccatga
     1921 tcgccgacaa attccgggca tggtgcgccg ggaagggcct cgctctcgat gcccggaaca
     1981 tcgaacaggc gttcagcagc ttctgcacca aggtgggccg ggtctgacca tcatcagttc
     2041 ggtggtcttc cgacgaacaa taaggccgca aatcgcggcc ttttttattg ataacaaaac
     2101 cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag cggcagcggc
     2161 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctactgg tggcaggata
     2221 tattgtggtg taaacaatgg agaaaaagag aattggaatt ccacctgcat atcagaggag
     2281 agagaccttt acggctagct cagtcctagg tatagtgcta gctactagag aaagaggaga
     2341 aatactaaat gtcgcactca aaacaagcac tggcagatac gatgaaaatg acgtggctga
     2401 tggaaggttc ggtcaacggt cacgccttta cgattgaagg cgaaggtacc ggcaaaccgt
     2461 atgaaggtaa acagagtggc acctttcgtg ttacgaaagg cggtccgctg ccgtttgcgt
     2521 ttgatattgt cgctccgacc ctgaaatacg gtttcaaatg cttcatgaaa tacccggcgg
     2581 atattccgga ctactttaaa ctggccttcc cggaaggcct gacgtacgat cgtaaaattg
     2641 cgtttgaaga cggcggttgt gcgaccgcca cggtcgaaat gagcctgaag ggtaacaccc
     2701 tggtgcataa aacgaacttt cagggcggta atttcccgat tgatggtccg gtgatgcaaa
     2761 aacgcaccct gggctgggaa ccgacctctg aaaaaatgac gccgtgcgat ggtattatca
     2821 aaggcgacac catcatgtac ctgatggttg aaggcggtaa aacgctgaaa tgtcgttatg
     2881 aaaacaatta ccgcgccaac aaaccagtgc tgatgccgcc gagccacttt gtggatctgc
     2941 gtctgacccg cacgaatctg gataaagaag gtctggcgtt caaactggaa gaatatgctg
     3001 ttgcccgtgt gctggaagtg taataagctt caaataaaac gaaaggctca gtcgaaagac
     3061 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     3121 tcaccttcgg gtgggccttt ctgcgggtct cacgctgtca atatgcaggt gctgcaggaa
     3181 ttgttgattt tgtgatgact gatggcagga tatatgcggt tgtaattcat ttttattgtc
     3241 taaatttctg tatttgtttg tttgttcggt tgtaaatttt tttggaagaa caagaaaaga
     3301 aaaaacaccc gttagggtgt ttttagttag tgtggcgcgc cgacttgcga catgcggtcc
     3361 tttgcaatca actattagaa aaattcatcc agcatcagat gaaattgcag tttgttcata
     3421 tccggattat caatgccata tttctgaaac agacgttttt gcaggctcgg gctaaattcg
     3481 cccaggcagt tccacagaat ggccagatcc tgataacgat ccgcaatgcc cacacggccc
     3541 acatcaatgc agccaatcag tttgccttca tcgaaaatca ggttatccag gctaaaatcg
     3601 ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt tatgcatttc tttccacacc
     3661 tgttccaccg gccagccgtt acgttcatca tcaaaatcgc tcgcatccac caggccgttg
     3721 ttcatacggc tctgcgcctg ggccagacga aacacacgat cgctgttaaa cgggcagttg
     3781 cacaccggaa tgctatgcag acgacgcaga aacacggcca gcgcatccac aatgttttcg
     3841 ccgctatccg gatattcttc cagcacctga aacgcggttt tgcccggaat cgcggtggtc
     3901 agcagccacg catcatccgg ggtgcgaata aaatgtttaa tggtcggcag cggcataaat
     3961 tcggtcagcc agttcagacg caccatttca tcggtcacat cgttcgccac gctgcctttg
     4021 ccatgtttca gaaacagttc cggcgcatcc ggtttgccat acagacgata aatggtcgcg
     4081 ccgctctgac ccacgttatc acgcgcccat ttatagccat acagatccgc atccatgttg
     4141 ctgttcagac gcggacggct acagctcgtt tcacgctgaa tatggctcat aacacccctt
     4201 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     4261 gcaatgtaac atcagagatt ttgagacaca ag
//
