LOCUS       sequence-317041-        4292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobius Assembly cloning and expression vector for plants with 
            high-copy number origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pMAP L1-Gamma
FEATURES             Location/Qualifiers
     source          1..4292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      60..648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     549..568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2170..2194
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    2221..2245
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2298..2332
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2341..2352
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3041..3112
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3128..3155
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3385..4200)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4192..4211
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gagacacaag atcggattgg cggttatgcg gttcccgtag aaaggatcaa aggatcttct
       61 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      181 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctgc cgatccgccg
      781 ccagttcagg ccgtccggcc ccggattctg accataattt catcgaaaaa aggggcgcag
      841 cccttcttgt tctaatagtt ctataagttc aggcgaaaat cgtgcagcaa ttacaaaagg
      901 ttgcgcgtct ataagtgggg aatccagacg caaaagtggg gaatccagcc gcaaaagtgg
      961 ggaatccagc cgaaatcgcg gattgacgag tgggatttcc cgcccaataa atccaccatg
     1021 ggaaagacac tcgacgttgc ccgcgaccgg gcctttgacc agaccgcgac cgtgctgccc
     1081 gccgaaatgg cgcggggggt ctatatgcgc aacgccccca gcctcgcggc cctgaagctg
     1141 atgcatctga tgatcgccac ggcgggcggg cgcatggccg atgacgtgcg ccacgaaatg
     1201 cggctggccg acatccgcaa gatcgacggt atggataacc acacccgggc cagcctgacc
     1261 ccgctctttg cggaactggc ggcggcggtg ctgacccacg acgacccgga aaagcgggtc
     1321 gtgaccatcg gtggcctgct ggacgaagcc cggatcgatt accggcacga ggtcagcggc
     1381 gatcttctgg tgtcgtggac cttccgcagc atgttccgcc gcatggcggc ggaatcgaac
     1441 cattgggcga ttctcgaccg gcagaccgtg ttccacctcg gcagcaagta ttccgtgctg
     1501 ttgttccagc acatcgccag cttcaaggaa tacgaccaca ttaccggcaa gacctttacc
     1561 gtgccggagt tgcgggctgt gtttggtatc cccgagggca aaatcaagcg tttcgcagac
     1621 ctcaacagag acgtgctgac gcccgccatt gccgaaatca accagctttc ccgcctgact
     1681 ctgaccgcca cgccgaacaa gatcgggcgc accgtggcca gcgtgacgat tgcttgggaa
     1741 gaaaagcccc tcgaaggcaa gcgctcgacc aaggccgaac tggaccgccc gaaggtgggc
     1801 cggaaggccc ggcgcgacgg caccgccgag acggtggcac gggccttccc ggcatcgggc
     1861 gggatcgagt tcgaccagca ttggcgcgac ctcaagcggg cggcgggctg caacatggac
     1921 aacaccatga tcgccgacaa attccgggca tggtgcgccg ggaagggcct cgctctcgat
     1981 gcccggaaca tcgaacaggc gttcagcagc ttctgcacca aggtgggccg ggtctgacca
     2041 tcatcagttc ggtggtcttc cgacgaacaa taaggccgca aatcgcggcc ttttttattg
     2101 ataacaaaac cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag
     2161 cggcagcggc ggcaggatat attcaattgt aaatggcttc atgtccggga aatctactgg
     2221 tggcaggata tattgtggtg taaacaatgg agaaaaagag aattggaatt ccacctgcat
     2281 atgtcaggag agagaccttt acggctagct cagtcctagg tatagtgcta gctactagag
     2341 aaagaggaga aatactaaat gtcgcactca aaacaagcac tggcagatac gatgaaaatg
     2401 acgtggctga tggaaggttc ggtcaacggt cacgccttta cgattgaagg cgaaggtacc
     2461 ggcaaaccgt atgaaggtaa acagagtggc acctttcgtg ttacgaaagg cggtccgctg
     2521 ccgtttgcgt ttgatattgt cgctccgacc ctgaaatacg gtttcaaatg cttcatgaaa
     2581 tacccggcgg atattccgga ctactttaaa ctggccttcc cggaaggcct gacgtacgat
     2641 cgtaaaattg cgtttgaaga cggcggttgt gcgaccgcca cggtcgaaat gagcctgaag
     2701 ggtaacaccc tggtgcataa aacgaacttt cagggcggta atttcccgat tgatggtccg
     2761 gtgatgcaaa aacgcaccct gggctgggaa ccgacctctg aaaaaatgac gccgtgcgat
     2821 ggtattatca aaggcgacac catcatgtac ctgatggttg aaggcggtaa aacgctgaaa
     2881 tgtcgttatg aaaacaatta ccgcgccaac aaaccagtgc tgatgccgcc gagccacttt
     2941 gtggatctgc gtctgacccg cacgaatctg gataaagaag gtctggcgtt caaactggaa
     3001 gaatatgctg ttgcccgtgt gctggaagtg taataagctt caaataaaac gaaaggctca
     3061 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     3121 tcacactggc tcaccttcgg gtgggccttt ctgcgggtct cacgctcttg atatgcaggt
     3181 gctgcaggaa ttgttgattt tgtgatgact gatggcagga tatatgcggt tgtaattcat
     3241 ttttattgtc taaatttctg tatttgtttg tttgttcggt tgtaaatttt tttggaagaa
     3301 caagaaaaga aaaaacaccc gttagggtgt ttttagttag tgtggcgcgc cgacttgcga
     3361 catgcggtcc tttgcaatca actattagaa aaattcatcc agcatcagat gaaattgcag
     3421 tttgttcata tccggattat caatgccata tttctgaaac agacgttttt gcaggctcgg
     3481 gctaaattcg cccaggcagt tccacagaat ggccagatcc tgataacgat ccgcaatgcc
     3541 cacacggccc acatcaatgc agccaatcag tttgccttca tcgaaaatca ggttatccag
     3601 gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt tatgcatttc
     3661 tttccacacc tgttccaccg gccagccgtt acgttcatca tcaaaatcgc tcgcatccac
     3721 caggccgttg ttcatacggc tctgcgcctg ggccagacga aacacacgat cgctgttaaa
     3781 cgggcagttg cacaccggaa tgctatgcag acgacgcaga aacacggcca gcgcatccac
     3841 aatgttttcg ccgctatccg gatattcttc cagcacctga aacgcggttt tgcccggaat
     3901 cgcggtggtc agcagccacg catcatccgg ggtgcgaata aaatgtttaa tggtcggcag
     3961 cggcataaat tcggtcagcc agttcagacg caccatttca tcggtcacat cgttcgccac
     4021 gctgcctttg ccatgtttca gaaacagttc cggcgcatcc ggtttgccat acagacgata
     4081 aatggtcgcg ccgctctgac ccacgttatc acgcgcccat ttatagccat acagatccgc
     4141 atccatgttg ctgttcagac gcggacggct acagctcgtt tcacgctgaa tatggctcat
     4201 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     4261 tttatcttgt gcaatgtaac atcagagatt tt
//
