LOCUS       sequence-317043-        3892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for plants with medium-copy number origin of 
            replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pLXMA L2A
FEATURES             Location/Qualifiers
     source          1..3892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(109..900)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1122..1784)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      1785..2556
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    2776..2800
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    2827..2851
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2894..2928
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2937..2948
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3679..3750
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3766..3793
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tttgtttgtt cggttgtaaa tttttttgga agaacaagaa aagaaaaaac acccgttagg
       61 gtgtttttag ttagtgtggc gcgccgactt gcgacatgcg gtcctttgtt atttgccgac
      121 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
      181 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
      241 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
      301 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
      361 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
      421 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
      481 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
      541 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
      601 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
      661 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
      721 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
      781 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
      841 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
      901 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
      961 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     1021 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     1081 gcgttcggtc aaggttcgat cggattggcg gttatgcggt tctaccggcg cggcagcgtt
     1141 acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa aacacggctc atcgggcatc
     1201 ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt cggtcaaggc tggcaggtct
     1261 ggttccatgc ccggaatgcc gggctggctg ggcggctcct cgccggggcc ggtcggtagt
     1321 tgctgctcgc ccggatacag ggtcgggatg cggcgcaggt cgccatgccc caacagcgat
     1381 tcgtcctggt cgtcgtgatc aaccaccacg gcggcactga acaccgacag gcgcaactgg
     1441 tcgcggggct ggccccacgc cacgcggtca ttgaccacgt aggccgacac ggtgccgggg
     1501 ccgttgagct tcacgacgga gatccagcgc tcggccacca agtccttgac tgcgtattgg
     1561 accgtccgca aagaacgtcc gatgagcttg gaaagtgtct tctggctgac caccacggcg
     1621 ttctggtggc ccatctgcgc cacgaggtga tgcagcagca ttgccgccgt gggtttcctc
     1681 gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg tttgcaccca gtgaccgggc
     1741 ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg ccatgcttat ctccatgcgg
     1801 taggggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga
     1861 caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa
     1921 tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg
     1981 atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac
     2041 ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc
     2101 cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg
     2161 gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt cgcccttcga
     2221 ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag ccgctgcgcg
     2281 gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag cgaagcgcgc
     2341 aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg ggcaaggccg
     2401 caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc ggtgggcaat
     2461 ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca gcagggttgt
     2521 ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc cacatatcca
     2581 cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca agcccgtagg
     2641 gggccatcat cagttcggtg gtcttccgac gaacaataag gccgcaaatc gcggcctttt
     2701 ttattgataa caaaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt
     2761 gcaaagcggc agcggcggca ggatatattc aattgtaaat ggcttcatgt ccgggaaatc
     2821 tactggtggc aggatatatt gtggtgtaaa caatggagaa aaagagaatt cggtctcagg
     2881 agatatgcag gtgtttacgg ctagctcagt cctaggtata gtgctagcta ctagagaaag
     2941 aggagaaata ctaaatgcgt aaaggcgaag aactgttcac gggcgtagtt ccgattctgg
     3001 tcgagctgga cggcgatgtg aacggtcata agtttagcgt tcgcggtgaa ggtgagggcg
     3061 acgcgaccaa cggcaaactg accctgaagt tcatctgcac caccggtaaa ctgccggtgc
     3121 cttggccgac cttggtgacg acgttgacgt atggcgtgca gtgttttgcg cgttatccgg
     3181 accacatgaa acaacacgat ttcttcaaat ctgcgatgcc ggagggttac gtccaggagc
     3241 gtaccatttc cttcaaggat gatggctact acaaaactcg cgcagaggtt aagtttgaag
     3301 gtgacacgct ggtcaatcgt atcgaattga agggtatcga ctttaaagag gatggtaaca
     3361 ttctgggcca taaactggag tataacttca acagccataa tgtttacatt acggcagaca
     3421 agcaaaagaa cggcatcaag gccaatttca agattcgcca caatgttgag gacggtagcg
     3481 tccaactggc cgaccattac cagcagaaca ccccaattgg tgacggtccg gttttgctgc
     3541 cggataatca ctatctgagc acccaaagcg tgctgagcaa agatccgaac gaaaaacgtg
     3601 atcacatggt cctgctggaa tttgtgaccg ctgcgggcat cacccacggt atggacgagc
     3661 tgtataagta atgagcttca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3721 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     3781 gggcctttct gcgcacctgc atataccctg caggaattgt tgattttgtg atgactgatg
     3841 gcaggatata tgcggttgta attcattttt attgtctaaa tttctgtatt tg
//
