LOCUS       sequence-31705-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC,
            Brock JE, Richardson AL, Weinberg RA
  TITLE     A pleiotropically acting microRNA, miR-31, inhibits breast cancer 
            metastasis.
  JOURNAL   Cell. 2009 Jun 12. 137(6):1032-46.
  PUBMED    19524507
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 12. 137(6):1032-46."
COMMENT     Sequence Label: pBabe-puro-mIR-31
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(438..458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        443..772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      623..758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(782..801)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ctgtcagtgt ggtggattgc ccttcatctt caaagcggac actctaagga agactatgtt
       61 gttatgatca gaaataagtg cgctttcaat taatgggtgt gttttccctc cctcaggtga
      121 aaggaaaaat tttggaaaag taaaacactg aagagtcata gtattctcct gtaacttgga
      181 actggagagg aggcaagatg ctggcatagc tgttgaactg ggaacctgct atgccaacat
      241 attgccatct ttcctgtctg acagcagcca tggccacctg catgccagtc cttcgtgtat
      301 tgctgtgtat gtgcgccctt ccttggatgt ggatttccat gacatggcct ttctcacctt
      361 ccttacttcc tgtcctgcta tgtattgtaa gggcaattct gcagatatcc agcacagtgg
      421 cggccgctcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac
      781 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacactccag c
//
