LOCUS       sequence-317089-        5929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LBS-tetO hybrid sponge with 20 repeats of LBS and 20 repeat of 
            Pecf11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Wan X, Pinto F, Yu L, Wang B
  TITLE     Synthetic protein-binding DNA sponge as a tool to tune gene 
            expression and mitigate protein toxicity.
  JOURNAL   Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9.
  PUBMED    33235249
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-24"; volume: "11(1):5961. doi"; pages: " 
            10.1038/s41467-020-19552-9"
COMMENT     Sequence Label: PXWS20LBS20Pecf11
FEATURES             Location/Qualifiers
     source          1..5929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(141..956)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(141..956)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     864..883
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     948..967
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(1551..2095)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1630..1649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2563..2581
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2585..2628)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2631..2652
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      5789..5860
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5876..5903
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    join(5910..5929,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
       61 tcactcaaag gcggtaatct cgagtcccgt caagtcagcg taatgctctg ccagtgttac
      121 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      181 ttcatatcag gattatcgat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      241 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      301 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      361 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
      421 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
      481 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
      541 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
      601 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
      661 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
      721 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
      781 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
      841 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
      901 atgttggaat ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg gctcataaca
      961 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     1021 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     1081 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     1141 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     1201 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     1261 ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca
     1321 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     1381 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     1441 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     1501 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     1561 ctccgccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
     1621 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc
     1681 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     1741 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     1801 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     1861 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     1921 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     1981 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcaaaaaac cgccctgcaa
     2041 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     2101 tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattccat ggtgccacct
     2521 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2581 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2641 ccgcttctag aggctcatac tgcgtaagag tctttaacat aagcacctgt aggatcgtac
     2701 aggtttacgc taggctcctt tgtcaaaagt gatcgctaac ataagcacct gtaggatcgt
     2761 acaggtttac gccttcacgg gataatgtgt tctacaacat aagcacctgt aggatcgtac
     2821 aggtttacgc gcttaagtca atccaatgcg ttcaggtgca acataagcac ctgtaggatc
     2881 gtacaggttt acgcaactcc gtcatcgtgg atgtcaatat tggcaacata agcacctgta
     2941 ggatcgtaca ggtttacgct tgatttcacc gctggcgtca atgagtgctc atactgcgta
     3001 agagtcttta acataagcac ctgtaggatc gtacaggttt acgctaggct cctttgtcaa
     3061 aagtgatcgc taacataagc acctgtagga tcgtacaggt ttacgccttc acgggataat
     3121 gtgttctaca acataagcac ctgtaggatc gtacaggttt acgcgcttaa gtcaatccaa
     3181 tgcgttcagg tgcaacataa gcacctgtag gatcgtacag gtttacgcaa ctccgtcatc
     3241 gtggatgtca atattggcaa cataagcacc tgtaggatcg tacaggttta cgcttgattt
     3301 caccgctggc gtcaatttcc gctcatactg cgtaagagtc tttaacataa gcacctgtag
     3361 gatcgtacag gtttacgcta ggctcctttg tcaaaagtga tcgctaacat aagcacctgt
     3421 aggatcgtac aggtttacgc cttcacggga taatgtgttc tacaacataa gcacctgtag
     3481 gatcgtacag gtttacgcgc ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct
     3541 gtaggatcgt acaggtttac gcaactccgt catcgtggat gtcaatattg gcaacataag
     3601 cacctgtagg atcgtacagg tttacgcttg atttcaccgc tggcgtcaat gactgctcat
     3661 actgcgtaag agtctttaac ataagcacct gtaggatcgt acaggtttac gctaggctcc
     3721 tttgtcaaaa gtgatcgcta acataagcac ctgtaggatc gtacaggttt acgccttcac
     3781 gggataatgt gttctacaac ataagcacct gtaggatcgt acaggtttac gcgcttaagt
     3841 caatccaatg cgttcaggtg caacataagc acctgtagga tcgtacaggt ttacgcaact
     3901 ccgtcatcgt ggatgtcaat attggcaaca taagcacctg taggatcgta caggtttacg
     3961 cttgatttca ccgctggcgt caattactag agatcgattt tagagctgcc gcctccacac
     4021 cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct gtactcagct
     4081 tatctcgggc ctccacaccg ctcgtcacat cctgtgatcc actcttcatc ccgctacgta
     4141 acacctctct taggtgtccc tagaatgagt atcgatttta gagctgccgc ctccacaccg
     4201 ctcgtcacat cctgtgatcc actcttcatc ccgctacgta acacctctgt actcagctta
     4261 tctcgggcct ccacaccgct cgtcacatcc tgtgatccac tcttcatccc gctacgtaac
     4321 acctctctta ggtgtcccta gaatttccat cgattttaga gctgccgcct ccacaccgct
     4381 cgtcacatcc tgtgatccac tcttcatccc gctacgtaac acctctgtac tcagcttatc
     4441 tcgggcctcc acaccgctcg tcacatcctg tgatccactc ttcatcccgc tacgtaacac
     4501 ctctcttagg tgtccctaga atgactatcg attttagagc tgccgcctcc acaccgctcg
     4561 tcacatcctg tgatccactc ttcatcccgc tacgtaacac ctctgtactc agcttatctc
     4621 gggcctccac accgctcgtc acatcctgtg atccactctt catcccgcta cgtaacacct
     4681 ctcttaggtg tccctagaat tgatatcgat tttagagctg ccgcctccac accgctcgtc
     4741 acatcctgtg atccactctt catcccgcta cgtaacacct ctgtactcag cttatctcgg
     4801 gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct
     4861 cttaggtgtc cctagaatta ctagagatcg attttagagc tgccgcctcc acaccgctcg
     4921 tcacatcctg tgatccactc ttcatcccgc tacgtaacac ctctgtactc agcttatctc
     4981 gggcctccac accgctcgtc acatcctgtg atccactctt catcccgcta cgtaacacct
     5041 ctcttaggtg tccctagaat gagtatcgat tttagagctg ccgcctccac accgctcgtc
     5101 acatcctgtg atccactctt catcccgcta cgtaacacct ctgtactcag cttatctcgg
     5161 gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct
     5221 cttaggtgtc cctagaattt ccatcgattt tagagctgcc gcctccacac cgctcgtcac
     5281 atcctgtgat ccactcttca tcccgctacg taacacctct gtactcagct tatctcgggc
     5341 ctccacaccg ctcgtcacat cctgtgatcc actcttcatc ccgctacgta acacctctct
     5401 taggtgtccc tagaatgact atcgatttta gagctgccgc ctccacaccg ctcgtcacat
     5461 cctgtgatcc actcttcatc ccgctacgta acacctctgt actcagctta tctcgggcct
     5521 ccacaccgct cgtcacatcc tgtgatccac tcttcatccc gctacgtaac acctctctta
     5581 ggtgtcccta gaattgatat cgattttaga gctgccgcct ccacaccgct cgtcacatcc
     5641 tgtgatccac tcttcatccc gctacgtaac acctctgtac tcagcttatc tcgggcctcc
     5701 acaccgctcg tcacatcctg tgatccactc ttcatcccgc tacgtaacac ctctcttagg
     5761 tgtccctaga attactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     5821 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     5881 accttcgggt gggcctttct gcgtttatat actagtagcg gccgctgca
//
