LOCUS       sequence-317091-        2909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Norton PA, Hynes RO
  TITLE     Alternative splicing of chicken fibronectin in embryos and in normal
            and transformed cells.
  JOURNAL   Mol Cell Biol. 1987 Dec . 7(12):4297-307.
  PUBMED    2830487
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1987 Dec . 7(12):4297-307."
COMMENT     Sequence Label: pcFN-C (chick fibronectin)
FEATURES             Location/Qualifiers
     source          1..2909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(470..489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(471..489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(496..513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(496..512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(721..740)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     840..862
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(900..918)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        986..1090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1091..1159,1160..1951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1309..1328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2122..2710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2611..2630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2864..2881
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa
      241 tactcaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaaa
      301 ctctggtgcc agtgcttagg ctttggcagt ggtcacttca gatgcgattc ctctaagtgg
      361 tgccacgata atggtgtgaa ctacaagatt ggggagaagt gggaccggca gggggagaat
      421 ggccagatga tgagctgcac ctgcctcggg aatggaaaag gagaattcgc cctatagtga
      481 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      541 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      601 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      661 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      721 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      781 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      841 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
      901 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2881 ggaagcggaa gagcgcccaa tacgcaaac
//
