LOCUS       sequence-317104-       11432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human ACE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Daniloski Z, Jordan TX, Wessels HH, Hoagland DA, Kasela S, Legut M, 
            Maniatis S, Mimitou EP, Lu L, Geller E, Danziger O, Rosenberg BR, 
            Phatnani H, Smibert P, Lappalainen T, tenOever BR, Sanjana NE
  TITLE     Identification of Required Host Factors for SARS-CoV-2 Infection in 
            Human Cells.
  JOURNAL   Cell. 2020 Oct 24. pii: S0092-8674(20)31394-5. doi: 
            10.1016/j.cell.2020.10.030.
  PUBMED    33147445
REFERENCE   2  (bases 1 to 11432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Oct 24. pii: S0092-8674(20)31394-5. doi: 
            10.1016/j.cell.2020.10.030."
COMMENT     Sequence Label: pHR_PGK-hACE2
FEATURES             Location/Qualifiers
     source          1..11432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     misc_feature    3070..3658
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3123..3143)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3541..3552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3661..3677)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3662..3678)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3768..4001
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4026..4044)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4027..4044)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4334..4353)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4567..4586)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4686..4708
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4746..4764)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4832..4936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4937..5005,5006..5797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5155..5174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5968..6556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6457..6476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6710..6727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6797..6817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6802..7131
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6982..7117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8265..8330
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8460..8480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8905..9039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8942..8961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8996..9015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9207..9840
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9887..10012
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10509..10742
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10927..10971
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11256..11373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      541 gccaccatgt caagctcttc ctggctcctt ctcagccttg ttgctgtaac tgctgctcag
      601 tccaccattg aggaacaggc caagacattt ttggacaagt ttaaccacga agccgaagac
      661 ctgttctatc aaagttcact tgcttcttgg aattataaca ccaatattac tgaagagaat
      721 gtccaaaaca tgaataatgc tggggacaaa tggtctgcct ttttaaagga acagtccaca
      781 cttgcccaaa tgtatccact acaagaaatt cagaatctca cagtcaagct tcagctgcag
      841 gctcttcagc aaaatgggtc ttcagtgctc tcagaagaca agagcaaacg gttgaacaca
      901 attctaaata caatgagcac catctacagt actggaaaag tttgtaaccc agataatcca
      961 caagaatgct tattacttga accaggtttg aatgaaataa tggcaaacag tttagactac
     1021 aatgagaggc tctgggcttg ggaaagctgg agatctgagg tcggcaagca gctgaggcca
     1081 ttatatgaag agtatgtggt cttgaaaaat gagatggcaa gagcaaatca ttatgaggac
     1141 tatggggatt attggagagg agactatgaa gtaaatgggg tagatggcta tgactacagc
     1201 cgcggccagt tgattgaaga tgtggaacat acctttgaag agattaaacc attatatgaa
     1261 catcttcatg cctatgtgag ggcaaagttg atgaatgcct atccttccta tatcagtcca
     1321 attggatgcc tccctgctca tttgcttggt gatatgtggg gtagattttg gacaaatctg
     1381 tactctttga cagttccctt tggacagaaa ccaaacatag atgttactga tgcaatggtg
     1441 gaccaggcct gggatgcaca gagaatattc aaggaggccg agaagttctt tgtatctgtt
     1501 ggtcttccta atatgactca aggattctgg gaaaattcca tgctaacgga cccaggaaat
     1561 gttcagaaag cagtctgcca tcccacagct tgggacctgg ggaagggcga cttcaggatc
     1621 cttatgtgca caaaggtgac aatggacgac ttcctgacag ctcatcatga gatggggcat
     1681 atccagtatg atatggcata tgctgcacaa ccttttctgc taagaaatgg agctaatgaa
     1741 ggattccatg aagctgttgg ggaaatcatg tcactttctg cagccacacc taagcattta
     1801 aaatccattg gtcttctgtc acccgatttt caagaagaca atgaaacaga aataaacttc
     1861 ctgctcaaac aagcactcac gattgttggg actctgccat ttacttacat gttagagaag
     1921 tggaggtgga tggtctttaa aggggaaatt cccaaagacc agtggatgaa aaagtggtgg
     1981 gagatgaagc gagagatagt tggggtggtg gaacctgtgc cccatgatga aacatactgt
     2041 gaccccgcat ctctgttcca tgtttctaat gattactcat tcattcgata ttacacaagg
     2101 accctttacc aattccagtt tcaagaagca ctttgtcaag cagctaaaca tgaaggccct
     2161 ctgcacaaat gtgacatctc aaactctaca gaagctggac agaaactgtt caatatgctg
     2221 aggcttggaa aatcagaacc ctggacccta gcattggaaa atgttgtagg agcaaagaac
     2281 atgaatgtaa ggccactgct caactacttt gagcccttat ttacctggct gaaagaccag
     2341 aacaagaatt cttttgtggg atggagtacc gactggagtc catatgcaga ccaaagcatc
     2401 aaagtgagga taagcctaaa atcagctctt ggagataaag catatgaatg gaacgacaat
     2461 gaaatgtacc tgttccgatc atctgttgca tatgctatga ggcagtactt tttaaaagta
     2521 aaaaatcaga tgattctttt tggggaggag gatgtgcgag tggctaattt gaaaccaaga
     2581 atctccttta atttctttgt cactgcacct aaaaatgtgt ctgatatcat tcctagaact
     2641 gaagttgaaa aggccatcag gatgtcccgg agccgtatca atgatgcttt ccgtctgaat
     2701 gacaacagcc tagagtttct ggggatacag ccaacacttg gacctcctaa ccagccccct
     2761 gtttccatat ggctgattgt ttttggagtt gtgatgggag tgatagtggt tggcattgtc
     2821 atcctgatct tcactgggat cagagatcgg aagaagaaaa ataaagcaag aagtggagaa
     2881 aatccttatg cctccatcga tattagcaaa ggagaaaata atccaggatt ccaaaacact
     2941 gatgatgttc agacctcctt ttagcttgac ttgcggccgc aactcccacc tgcaacatgc
     3001 gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     3061 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3121 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3181 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3241 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3301 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3361 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3421 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3481 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3541 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3601 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3661 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     3721 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     3781 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     3841 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3901 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3961 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     4021 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     4081 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     4141 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     4201 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     4261 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     4321 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     4381 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     4441 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     4501 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     4561 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     4621 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4681 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     4741 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4801 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4861 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4921 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4981 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5041 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5101 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5161 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5221 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5281 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5341 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5401 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5461 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5521 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5581 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5641 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5701 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5761 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6661 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6721 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6781 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6961 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7081 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     7141 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     7201 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     7261 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     7321 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     7381 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     7441 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     7501 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     7561 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     7621 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     7681 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     7741 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     7801 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     7861 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     7921 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     7981 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     8041 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     8101 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     8161 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     8221 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     8281 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     8341 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     8401 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     8461 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     8521 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     8581 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     8641 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     8701 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     8761 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     8821 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8881 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9001 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     9061 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     9121 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9181 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     9241 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     9301 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     9361 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     9421 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     9481 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     9541 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     9601 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     9661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9841 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     9901 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     9961 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    10021 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    10081 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    10141 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    10201 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    10261 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    10321 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
    10381 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    10441 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    10501 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    10561 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    10621 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    10681 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    10741 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10801 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10861 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    10921 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    10981 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    11041 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    11101 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    11161 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    11221 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
    11281 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    11341 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    11401 ccagtttgga tcgataagct tgatatcgaa tt
//
