LOCUS       sequence-317165-        3313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of DNA fragment for nucleosome core particle preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Sharma D, De Falco L, Padavattan S, Rao C, Geifman-Shochat S, Liu 
            CF, Davey CA
  TITLE     PARP1 exhibits enhanced association and catalytic efficiency with 
            gammaH2A.X-nucleosome.
  JOURNAL   Nat Commun. 2019 Dec 17;10(1):5751. doi: 10.1038/s41467-019-13641-0.
  PUBMED    31848352
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-17"; volume: "10(1):5751. doi"; pages: " 
            10.1038/s41467-019-13641-0"
COMMENT     Sequence Label: Sticky-147-4X
FEATURES             Location/Qualifiers
     source          1..3313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(812..829)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(812..828)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(821..843)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1037..1056)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1156..1178
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1216..1234)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1302..1406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1407..1475,1476..2267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1625..1644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2438..3026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2927..2946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3180..3197
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cttaaaaaaa gatctaaagg taccatatat cccggtgccg aggccgctca
      181 attggtcgta gacagctcta gcaccgctta aacgcacgta cgcgctgtct accgcgtttt
      241 aaccgccact agaagcgctt actagtctcc aggcacgtgt gagaccggca tatatggtac
      301 caaaggatct aaaggtacca tatatcccgg tgccgaggcc gctcaattgg tcgtagacag
      361 ctctagcacc gcttaaacgc acgtacgcgc tgtctaccgc gttttaaccg ccactagaag
      421 cgcttactag tctccaggca cgtgtgagac cggcatatat ggtaccaaag gatctaaagg
      481 taccatatat cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta
      541 aacgcacgta cgcgctgtct accgcgtttt aaccgccact agaagcgctt actagtctcc
      601 aggcacgtgt gagaccggca tatatggtac caaaggatct aaaggtacca tatatcccgg
      661 tgccgaggcc gctcaattgg tcgtagacag ctctagcacc gcttaaacgc acgtacgcgc
      721 tgtctaccgc gttttaaccg ccactagaag cgcttactag tctccaggca cgtgtgagac
      781 cggcatatat ggtaccaaag gatccgaatt cactggccgt cgttttacaa cgtcgtgact
      841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      961 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     1021 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     1081 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     1141 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     1201 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1261 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1321 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1381 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1441 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1501 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1561 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1621 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1681 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1741 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1801 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1861 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1921 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1981 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2041 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2101 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2161 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2221 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2281 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2581 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3181 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3301 agcgcaacgc aat
//
