LOCUS       sequence-317173-        4043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of DNA fragment for nucleosome core particle preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Chua EY, Vasudevan D, Davey GE, Wu B, Davey CA
  TITLE     The mechanics behind DNA sequence-dependent properties of the 
            nucleosome.
  JOURNAL   Nucleic Acids Res. 2012 Jul;40(13):6338-52. doi: 10.1093/nar/gks261.
            Epub 2012 Mar 26.
  PUBMED    22453276
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks261"; journalName: "Nucleic Acids Res"; date: 
            "2012-07"; volume: "40"; issue: "13"; pages: "6338-52"
COMMENT     Sequence Label: 601L-145-8X
FEATURES             Location/Qualifiers
     source          1..4043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1444..1460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1444..1460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1457..1479)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1468..1484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1492..1498,1499..1516,1517..1522))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1537..1558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1675..1692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1846..2434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1926..1945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2605..3396,3397..3465))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3228..3247
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3466..3570)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3638..3656
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3694..3716)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3816..3835
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(4029..4043,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4043,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttcgagctcg gtacctcgcg aatgcatcta gagatatcac
       61 aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac
      121 gtacggaatc agttccgata tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac
      181 agctctagca ccgcttaaac gcacgtacgg attcagttcc gatatcacaa tcccggtgcc
      241 gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt acggaatcag
      301 ttccgatatc acaatcccgg tgccgaggcc gctcaattgg tcgtagacag ctctagcacc
      361 gcttaaacgc acgtacggat tcagttccga tatcacaatc ccggtgccga ggccgctcaa
      421 ttggtcgtag acagctctag caccgcttaa acgcacgtac ggaatcagtt ccgatatcac
      481 aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac
      541 gtacggattc agttccgata tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac
      601 agctctagca ccgcttaaac gcacgtacgg aatcagttcc gatatcacaa tcccggtgcc
      661 gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt acggattcag
      721 ttccgatatc acaatcccgg tgccgaggcc gctcaattgg tcgtagacag ctctagcacc
      781 gcttaaacgc acgtacggaa tcagttccga tatcacaatc ccggtgccga ggccgctcaa
      841 ttggtcgtag acagctctag caccgcttaa acgcacgtac ggattcagtt ccgatatcac
      901 aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac
      961 gtacggaatc agttccgata tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac
     1021 agctctagca ccgcttaaac gcacgtacgg attcagttcc gatatcacaa tcccggtgcc
     1081 gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt acggaatcag
     1141 ttccgatatc acaatcccgg tgccgaggcc gctcaattgg tcgtagacag ctctagcacc
     1201 gcttaaacgc acgtacggat tcagttccga tatcacaatc ccggtgccga ggccgctcaa
     1261 ttggtcgtag acagctctag caccgcttaa acgcacgtac ggaatcagtt ccgatatcac
     1321 aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac
     1381 gtacggattc agttccgata tcgtcgactg cagaggcctg catgcaagct tggcgtaatc
     1441 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1501 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1561 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1621 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1681 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1741 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1801 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1861 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1921 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1981 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2041 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2101 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2161 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2221 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2281 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2341 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2401 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2461 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2521 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2581 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2641 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2701 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2761 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2821 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2881 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2941 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3001 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3061 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3121 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3181 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3241 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3301 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3361 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3421 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3481 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3541 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3601 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     3661 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3721 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3781 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     3841 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     3901 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3961 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     4021 agggttttcc cagtcacgac gtt
//
