LOCUS       sequence-317181-        4040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of DNA fragment for nucleosome core particle preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Chua EY, Vasudevan D, Davey GE, Wu B, Davey CA
  TITLE     The mechanics behind DNA sequence-dependent properties of the 
            nucleosome.
  JOURNAL   Nucleic Acids Res. 2012 Jul;40(13):6338-52. doi: 10.1093/nar/gks261.
            Epub 2012 Mar 26.
  PUBMED    22453276
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks261"; journalName: "Nucleic Acids Res"; date: 
            "2012-07"; volume: "40"; issue: "13"; pages: "6338-52"
COMMENT     Sequence Label: 601R-145-8X
FEATURES             Location/Qualifiers
     source          1..4040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1441..1457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1441..1457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1454..1476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1465..1481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1489..1495,1496..1513,1514..1519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1534..1555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1672..1689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1843..2431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1923..1942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2602..3393,3394..3462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3225..3244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3463..3567)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3635..3653
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3691..3713)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3813..3832
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(4026..4040,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4040,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttcgagctcg gtacctcgcg aatgcatcta gagatatcga
       61 tgaatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg ttaaaacgcg
      121 ggggagaatc agttccgata tcgatgaata tatctgacac gtgcctggag actagggagt
      181 aatccccttg gcggttaaaa cgcgggggag attcagttcc gatatcgatg aatatatctg
      241 acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg ggagaatcag
      301 ttccgatatc gatgaatata tctgacacgt gcctggagac tagggagtaa tccccttggc
      361 ggttaaaacg cgggggagat tcagttccga tatcgatgaa tatatctgac acgtgcctgg
      421 agactaggga gtaatcccct tggcggttaa aacgcggggg agaatcagtt ccgatatcga
      481 tgaatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg ttaaaacgcg
      541 ggggagattc agttccgata tcgatgaata tatctgacac gtgcctggag actagggagt
      601 aatccccttg gcggttaaaa cgcgggggag aatcagttcc gatatcgatg aatatatctg
      661 acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg ggagattcag
      721 ttccgatatc gatgaatata tctgacacgt gcctggagac tagggagtaa tccccttggc
      781 ggttaaaacg cgggggagaa tcagttccga tatcgatgaa tatatctgac acgtgcctgg
      841 agactaggga gtaatcccct tggcggttaa aacgcggggg agattcagtt ccgatatcga
      901 tgaatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg ttaaaacgcg
      961 ggggagaatc agttccgata tcgatgaata tatctgacac gtgcctggag actagggagt
     1021 aatccccttg gcggttaaaa cgcgggggag attcagttcc gatatcgatg aatatatctg
     1081 acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg ggagaatcag
     1141 ttccgatatc gatgaatata tctgacacgt gcctggagac tagggagtaa tccccttggc
     1201 ggttaaaacg cgggggagat tcagttccga tatcgatgaa tatatctgac acgtgcctgg
     1261 agactaggga gtaatcccct tggcggttaa aacgcggggg agaatcagtt ccgatatcga
     1321 tgaatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg ttaaaacgcg
     1381 ggggagattc agttccgata tcgrmtscmg nggccygymk rcwrsmkwgg cgtaatcatg
     1441 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1501 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1561 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1621 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1681 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1741 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1801 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1861 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1921 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1981 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2041 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2101 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2161 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2221 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2281 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2341 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2401 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2461 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2521 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2581 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2641 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2701 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2761 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2821 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2881 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2941 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3001 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3061 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3121 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3181 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3241 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3301 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3361 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3421 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3481 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3541 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3601 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3661 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3721 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3781 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3841 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     3901 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3961 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4021 gttttcccag tcacgacgtt
//
