LOCUS       sequence-317221-        2790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express SV-Tag construct in a Cre-independent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini 
            BL
  TITLE     Rapid purification and metabolomic profiling of synaptic vesicles 
            from mammalian brain.
  JOURNAL   Elife. 2020 Oct 12;9. pii: 59699. doi: 10.7554/eLife.59699.
  PUBMED    33043885
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-12"; volume: "9. pii: 59699. doi"; pages: " 
            10.7554/eLife.59699"
COMMENT     Sequence Label: pAAV hSynapsin SV-tag
FEATURES             Location/Qualifiers
     source          1..2790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     596..619
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             1435..1488
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2449..2475
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2488..2514
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2527..2553
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2566..2592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2605..2631
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2644..2670
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2683..2709
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2722..2748
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2761..2787
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      241 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      361 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      541 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      661 cgccaccacc tgttcctggg gcatggtacc ggcagcaccg gcagcggcag ctccggcacc
      721 gcctcctccg aggacaacaa catggccgtg attaaggagt ttatgaggtt taaagtcagg
      781 atggagggca gcatgaacgg gcacgagttt gagattgaag gagaggggga agggaggccc
      841 tatgagggca cccagacagc aaagctgaaa gtgacaaagg gcggccctct gccattcgca
      901 tgggacatcc tgtctcctca gtttatgtat ggaagtaaag cctacgtgaa acaccctgct
      961 gacattccag attacaagaa actgagcttc ccagaggggt ttaagtggga aagagtgatg
     1021 aacttcgaag acgggggcct ggtgactgtc acccaggaca gctctctgca ggacggcact
     1081 ctgatctata aggtgaaaat gcgcggcacc aattttcccc ctgatggccc agtcatgcag
     1141 aagaagacaa tgggctggga ggcttcaaca gaaaggctgt accccagaga cggcgtgctg
     1201 aagggagaga tccaccaggc actgaagctg aaagatggag ggcattatct ggtcgagttc
     1261 aaaaccatct acatggccaa gaaacccgtg cagctgcctg gctactatta cgtcgacaca
     1321 aagctggata ttactagcca taacgaagac tacaccattg tggagcagta tgagcggagc
     1381 gaggggaggc accatctgtt tctgtacggc atggacgagc tgtacaagac tagtgagggc
     1441 agaggaagtc ttctgacatg cggagacgtg gaagagaatc ccggccctgc cccaggctca
     1501 tctagaatgg acgtggtgaa tcagctggtg gctgggggtc agttccgggt ggtcaaggag
     1561 ccccttggct tcgtgaaggt gctgcagtgg gtctttgcca tcttcgcctt tgctacgtgc
     1621 ggcagctaca ccggagagct tcggctgagc gtggagtgtg ccaacaagac ggagagtgcc
     1681 ctcaacatcg aagtcgaatt tgagtaccca ttcaggctgc accaagtgta ctttgatgca
     1741 ccctcctgcg ttaaaggggg cactaccaag atcttcctag ttggtgacta ctcctcctcg
     1801 gctgagttct ttgtcaccgt ggctgtgttt gccttcctct actccatggg ggccctggcc
     1861 acctacatct tcctgcagaa caagtaccga gagaacaaca aagggccaat gatggacttc
     1921 ctggccacag cagtgttcgc tttcatgtgg ctagttagct catccgcctg ggccaaaggc
     1981 ctgtccgatg tgaagatggc cactgaccca gagaacatta tcaaggagat gcctatgtgc
     2041 cgccagacag gaaacacatg caaggaactg agggaccctg tgacttcagg actcaacacc
     2101 tcggtggtgt ttggcttcct gaacctggtg ctctgggttg gcaacctatg gttcgtgttc
     2161 aaggagacag gctgggccgc cccattcatg cgcgcacctc caggcgcccc agaaaagcaa
     2221 ccagctcctg gcgatgccta cggcgatgcg ggctatgggc agggccccgg aggctatggg
     2281 ccccaggact cctacgggcc tcagggtggt tatcaacccg attacgggca gccagccagc
     2341 ggcggtggcg gtggctacgg gcctcagggc gactatgggc agcaaggcta cggccaacag
     2401 ggtgcgccca cctccttctc caatcagatg tcgcgaggag ggagcggcta tccctatgac
     2461 gtgcctgatt acgccggcac aggatcctac ccctatgatg tgcctgacta cgctggcagc
     2521 gccggatacc cttatgatgt gcctgattat gctggaggga gcggctaccc atacgatgtt
     2581 ccagattacg ctggaacagg cagctatccc tatgacgtcc cggactatgc aggcagcgcc
     2641 ggatatccat atgacgttcc agattacgct ggaggctccg gctaccctta tgatgtgccc
     2701 gattatgccg gaacaggcag ctatccctat gacgtgcctg actatgctgg atccgccggc
     2761 tacccttatg acgtgcccga ttacgcctga
//
