LOCUS       sequence-317225-       14480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at 
            ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Adikes RC, Kohrman AQ, Martinez MAQ, Palmisano NJ, Smith JJ, 
            Medwig-Kinney TN, Min M, Sallee MD, Ahmed OB, Kim N, Liu S, Morabito
            RD, Weeks N, Zhao Q, Zhang W, Feldman JL, Barkoulas M, Pani AM, 
            Spencer SL, Martin BL, Matus DQ
  TITLE     Visualizing the metazoan proliferation-quiescence decision in vivo.
  JOURNAL   Elife. 2020 Dec 22;9. pii: 63265. doi: 10.7554/eLife.63265.
  PUBMED    33350383
REFERENCE   2  (bases 1 to 14480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-12-22"; volume: "9. pii: 63265. doi"; pages: " 
            10.7554/eLife.63265"
COMMENT     Sequence Label: pWZ157
FEATURES             Location/Qualifiers
     source          1..14480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1624..1657)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1657..1668)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3090..3413)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5331..5355
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5358..6383
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5358..6383
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6384..6408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6761..6794)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7163..7180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7164..7180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10176..10256
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      10265..10588
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(10621..10637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10621..10637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(12110..12130)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(12267..12855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12347..12366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13026..13817,13818..13886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13649..13668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13887..13991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14059..14077
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14115..14137)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14237..14256
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    14460..14480
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcagtag gaacgtgtgt
      181 aggtataggc aggcattatc gcgtctcctt tcgaactacc tagtacctag taggtagttt
      241 gaagggaggc gcgataatgc ctgcctatgt ctacacatgt gcctactgcg catggaaata
      301 ggcggtagtc aggcaggtag atagcctcat aaaattcaat acttcctttt cagaaagaat
      361 gctggaatgt attcaaagag gagtcactct tcccattgct cagagaatat gaagagttgc
      421 tcggcgagca aaagttcatg attggagatc catattcatg ggctgacatc gctgtcgtcg
      481 agtttctaac tcggtgtcag cagtgctacg atagcttcta tctggctcat ttcgagaggc
      541 tccgaacttt ttgccagact tttgagtctc ttccgcacat tcgtccctac attcagggac
      601 gtgttgattc atttatctaa ttttttaaaa tggatatttt tcgtttttta tttgtttgtt
      661 ttgctgataa ttcaataaag ttacgcaacg aattttagac atttatgaaa accgaaaatc
      721 gacaaaaata ttttaagaaa aatagcagca aaattcaata ttttatcaaa ttttgtttat
      781 ggtaatgaga aagaaaacat acaatatttt tctaagaaga agaaaaatga ccagaatttt
      841 aagatttttc tcgaatatac gaaaaattga taaactaaaa ttttcgattt ttccatttat
      901 tttgaaaaaa aaactcttga cggttttcac catgcgaaga ggtgagaaat attctgaaag
      961 ttatgcattt tgaaaaaatt gcaaaaaaat acgttaaaat tttttgatct attgtttttt
     1021 tttgaaaacc ggaatttaaa atggaatttt ttttcaaaaa ttaaaattaa aatttaaata
     1081 tttctagtgt ttggatagtt tttcaaaagc tagatttaac ccgcgagatg tcgaccgcta
     1141 gtgtagctta cagatggctg aagacttcgc aaaaatctta tcttagaaac ccgcgaggtg
     1201 tcggccgcta aaactttgta aacctgccaa caaaatgctt cgcattgtct gaacagatga
     1261 aaccttgaaa caaaacatat acaattgaaa tcccgtaaag tgtcgggcgc aagaactgcg
     1321 attagttgta taattggact agctcaaatt tgaccaaaat ctcattttta gacctctaag
     1381 atgtcggcca ctataacttt attcaacttg aaactgcaaa aaaacttatc aactgtatag
     1441 attcaagtat tatgtcaagt attggtttta gagctacagt acactctgat aacaaaatct
     1501 gcagaaatac ctccctgtca attcccaaaa tacctcccct catctcaatt atcccgaaaa
     1561 tcggttagtc attgccatca gaaaatcgaa ttaggtacca tgatggtagc aaactcactt
     1621 cgtataactt cgtatagtat accttatacg aagttatctg ccctcgattg tctatcttcc
     1681 attctcacca agtttcaaca actttattca gtcattcttc tctggttcat ttcccaacca
     1741 atcttcccga ataaagccca tctgtccttt cggccacatt cctcacctac atccgtactc
     1801 cttatctccc gttcttatcc agtcttttat cgtctctgcg tctcgcacac tgtctcgtcg
     1861 gtaatgtaaa aaaaaaacaa aaaaaacttg aaaacttgcg gaaagatttc ctaataaaag
     1921 gagacgaatc cggtaggaaa tcgacaagtc tctgcttctc gaagactagg tgagttacat
     1981 ttctttggcc gaattatttt gaaatcctaa atttgataat ttcagagatg tctgtaaaac
     2041 ttgcgtgtta tgtgacggct tcggtcactg tcgccactct tatggtatgc ttcatgacca
     2101 tgtcaaccat ctactcggag gttgatggat tcagagagaa gcttgatacc gagatgaatg
     2161 tgttcagagt gagttaaatt atattttgaa ttttaaataa tttttaattt tctagcaatc
     2221 taccaatgga ttgtggaagg acatagttgt catcggaaga tctagcaagc gtgtccgttg
     2281 tcaatatgaa gagaccaacg ctaccccaac tccacatgct gatggatccc catctgctcc
     2341 accaggtcaa ccaccagcag ttccaccagt cttcaaccag ccaaagactc caaatggagc
     2401 caatggaaat ggaccaacct gcaactgcaa tgctgataac aagtgcccag ctggaccatc
     2461 cggaccaaag ggagttccag gagttccagg actcgacgga gttccaggac ttgacggtgt
     2521 tccaggagtt ggagctgatg atatcgctcc acaacgcgag tctgtcggat gcttcacttg
     2581 cccacaagga ccagttggac caccaggagc tcttggaaga ccaggaccac gtggacttcc
     2641 aggaccaaga ggacaaaatg gaaacccagg aagagatgga caaccaggac atccaggaga
     2701 gcaaggatca tccggtcaaa tcggaaagat cggagagcca ggaccaccag gagagaaggg
     2761 acgcgacgcc gagcatccaa tcggaagacc aggaccaaag ggaccaagag gagatcaagg
     2821 accaacagga ccagctggac agaacggtct tcacggacca ccaggagagc caggaaccgt
     2881 tggaccagaa ggaccatctg gaaagcaagg acgtcaagga ccagacggaa cccagggaga
     2941 gactggacca gacggaagac caggaaagga tgccgagtac tgccagtgcc cagacaagtc
     3001 tccaccatca gaggctgtca acgccaaccg tggatacaga aatatctaaa ttgttggtgt
     3061 tttctaataa aatatttgag attcagtgag acttttttct tggcggcaca ataaagtttc
     3121 ctctttttgt tgggtcactc aacgaacaca atacaaatat ttgagcgctc gcatttttct
     3181 caacctttcg ggtttaaagc acttcaaaat ggaattgaag agagattgtg aaagtacatc
     3241 agacagaatc acattttgat agcattcact tcactcagat gcaaactttt cagtgactat
     3301 aaaaagtcat aaaataataa cattaaaaaa gcaaataaat tagcgagaga attttttgac
     3361 aaaaagaaag aagagtgata gagaagaagg gaatgcttga aaggattttg catttatctt
     3421 aatcaccgtc ttcgagaaga cgaaccatgg cgccagtttc agaatccaag tttctaatat
     3481 agttcatcac aatgttgacg ttggtccaac ctccggcttg cataatctct gggatggaaa
     3541 ctccagcgcg agccatgtca cgggcagctc cgacacgggc ggaatgtcca gaccaagcca
     3601 agtaacgttg tccggaatca tccttggctc cgtagatgag acggtgggta gcttcgaaga
     3661 ttcctgaaaa tttattttca gcttattctt agctaatatg ttgtttaaac ttaccttcaa
     3721 gagctcgagt cgagagttgt gatgttgcac tcggggcagc aactccgttc ttgcgaacac
     3781 gacagaacag gtagttgttt ggatcatcgg cgactccgga aaccgaaatc cagcgctcaa
     3841 cgagcttggt aactccaagg gagagcgctt tttcgactcc ggcagtcgag accagcgttt
     3901 ttgtacgtcc gatgtggatg agcattcttc ctccgtctgt gcgactaata tccttgacgc
     3961 gaatgcgggc aatttcggcg atacggagaa gtgtgttgta agcaattccg aggaaggcga
     4021 ggtttcggat gtcctggcaa cgatcggaat tttccataag agaacggacc tgatcgaagt
     4081 cggttctgaa aatttcgtca agtcttagga ttgaatacat taatatttaa acttaccttt
     4141 cgaaggcgag ggcttgcttg gctctctctc cagcatcaac attttccttg cggatacgtc
     4201 tcattacgag agatacggca ttagaatctg atgggcgtgg aagtccagat cggcgatgga
     4261 gcatattgag ctgtccgaga tgttgttgga tcgtcttcac cgccaatcca cgagcttgga
     4321 ggtagagcaa gtagtcacga acgtcttctg gctcggctgg gaaccacttg cggttgttaa
     4381 gcttgcacca ggcagcccaa gaacggcaca ctgagagaag catcttccag gtgtgctcgg
     4441 agaaggcttg tctgtcacgg aacatatcca tgaggttctt gcgaacttca tcacttgtcg
     4501 cgtcaactgg aagtgctgga agattttgat gaacagtgag aagatttgac atattttcga
     4561 agttttttag atgcactaga acaaagcgtg ttggcttcct ctgagcccgc tttccttata
     4621 tacccgcatt ctgcagcctt acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa
     4681 atactcccgg tgggtgcaaa gagacgcaga cggaaaatgt atctgggtct ctttattgtg
     4741 tacactactt ttccatgtac cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa
     4801 tgttctagaa aaaggtggaa aatagtataa ataccgttga aaataaatac cgaacaacat
     4861 ttgctctaat tgtgaaatta gaaatcttca aactataatc atgtcacttt accactattt
     4921 ccgtccagct caacgttccg tttttggtga tcatttttgc tttcgtcgta aatctacaca
     4981 cgcgtctctt ccgtgcgaga gtccaagcca gcagccaaat tcgttgactg agtattcaac
     5041 gtttatacgt tgtcggcaac gagaaatagg aaaatgcatc gggaaatgtt cttttttcga
     5101 ttttttccaa ggttttgaca aattttacca cgaattttgc tatgttttca attaaaaaat
     5161 atgttattca actgtttcta tgaggaaaat aaggctttgc atgtaatttt cttattcagc
     5221 ataattttta attaatttga attttctgtc ctaacgttta ttttgttttc ttggttatga
     5281 ctgatctgaa attaattttt gaattttaag gtaatatgtc aggcggtgcc gcaagtttgt
     5341 acaaaaaagc aggctccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     5401 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     5461 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     5521 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     5581 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     5641 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     5701 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     5761 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     5821 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     5881 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     5941 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     6001 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     6061 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     6121 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     6181 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     6241 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     6301 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     6361 ctcgtccgag ggcaaaggaa tagacccagc tttcttgtac aaagtgggtc caattactct
     6421 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     6481 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     6541 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     6601 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     6661 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     6721 ttatcatatg ttacgtttca gtttatgacc gcaattttta ataacttcgt atagtatacc
     6781 ttatacgaag ttatactagt atatattttt atgttttctt ttaaaccttc ctccatattt
     6841 ctgaattgaa tgttgttgca tattgtgtgt ccttatttca tttccatcat ccttttgtgt
     6901 gtttccccct tccaccaatt ccccccacgt cctaatgaaa tgaaagcgag aagataaata
     6961 aacttgcaca ttgtgttcag atgagaggag cggaatacga attgggagac ggaaagagaa
     7021 aaaaaaatgt ttagatttgg attgaattaa ttttttagaa agcaagaaaa aaaacacaaa
     7081 gagaataaat aaataatagg aaaaaaaaca aatattcagt atttaaaatt tgaaaattca
     7141 acgacgttgg cgtcgatcat cctgtaaaac gacggccagt gcggccgcag aaacagtggc
     7201 cgtattggga aaaatgaacg atttttcggc gggaataatt tattttttta tgtttttcta
     7261 tgcgttttcg ggtgttttcg ggttgctaag cgattggttg cctttttgaa tcactagtct
     7321 tggtttttgt tgtttctgtg aatgaataat tggtttttcg aggttttttt gtcaaacatg
     7381 cctaaaaaat aaattatgac gttttagttg atttgtttgt tctttaaacg tctgaaaata
     7441 aggtttaaat ctaatttatt aattataaaa ttcgtcaaaa taagttgcgc gtcaaattat
     7501 atgtattgta cgcagtgtca aactccaggc ctcagttttc atgaatttac cagcgatttt
     7561 tgttataaat ttttttattg aaatttaaaa tttttatttt tcaaccaatt tgcctcgaaa
     7621 attcgttatt tccccattaa aaaccgcttt tctaaagtgt tgcgcgtcaa ataaaatgcc
     7681 tggtacgcaa tgcacggaga atgcgcaaag gacgactgct ggcgcacttt ttgaatgcgg
     7741 taaattgagg cgcgaagttt cattcgaaaa cgcgcgcgaa acttcattca tcgcactttc
     7801 tccgttcatt tcgtcctatt tttttgtggt ttttcgcgat tttttcgctt ttctgagtga
     7861 aaaaataatt ttccttcgtt ttttcaatga aaatccgcgg aaaacccatt ttttcccgtg
     7921 aaaatccgca tttttcgctg tatttcataa tttttattca gatctcccgt caaaatgttt
     7981 gaggccaagc tcgccaacgc cggactcctt aagaagatcg tcgaatcgat caaggatctc
     8041 gtcaccgatg ctccattcga ttgctccgag acggcgatga gccttcaggc tatggactcg
     8101 tctcacgtcg cgttggtttc gctcaagttg gaggtaaaat tgggaaaaat gaagaaaatt
     8161 agctaatttt ccgtgttcag gtcggccttt tcgacacata tcgctgcgat cggaccatca
     8221 acctcggact ttcgttggct aatatgtcga aggcgctcaa gtgcgccaac aacgacgata
     8281 cctgcatgct gaaggtgatt gtaaaagctt taaatcgaag aaatcagcat ttttcacctt
     8341 gaattgtgga ttttcatgcg aaaaatcgct tcattcggac tttttcgccc gaaaaatccg
     8401 aaaatgtggt ttttcggtag tcaaaagtgg caaaatcagt tttttttttc gcactaaaaa
     8461 tcattgaaaa ttaatatttt tagagacttt taggccgaaa tagtctgaat taatcatttt
     8521 tcgttctaaa aattattaga aattcacatt tttggcggaa atcagcattt ttcacccgaa
     8581 aaatcactgg aaactcaaat tgttatcgat ttttatgcca aaatagtccg agttttccat
     8641 tttatgcctt aaaaatcatt agaaaacaac atttccgcct gaaaaatcat tgaaaattca
     8701 aattttttat cgatttttcc ccgaaaaatt ccaattttaa gcccaaaaat taccaatttt
     8761 cattgcagta tgaggaaaac gaaggcgact cgatcatctt cacattcgcc gatccaaagc
     8821 gtgacaagac ccaagacgtg acagtcaaaa tgatggacat tgacagcgaa catctcggga
     8881 tcccggatca agactacgcc gtcgtttgtg agatgcctgc cggcgagttc cagaagactt
     8941 gcaaggatct gtcgacgttc tccgactcgt tgaacatcac cgccaccaag gctggcatcg
     9001 tgttcaccgg aaagggagac atcggatctt ctgtcgtcac ctactcccca tcttccaaca
     9061 ctgacgatga gactgaggct gtaacactgg aggtcaagga tccggttaat gtgaacttct
     9121 cgatcaagta catgaatcag ttcaccaagg cgacggcact ttccgacaga gttcgtctct
     9181 ctctgtgcaa cgatgttccg gtcgttgtcg agtatccgat cgaggagaat ggatatcttc
     9241 gcttctactt ggctccaaag attgacgacg acgagaatat ggacggagca tcgatgagta
     9301 aaggagaaga attgttcact ggagttgtcc caatcctcgt cgagctcgac ggagacgtca
     9361 acggacacaa gttctccgtc tccggagagg gagagggaga cgccacctac ggaaagctca
     9421 ccctcaagtt catctgcacc accggaaagc tcccagtccc atggccaacc ctcgtcacca
     9481 ccttctgcta cggagtccaa tgcttctccc gttacccaga ccacatgaag cgtcacgact
     9541 tcttcaagtc cgccatgcca gagggatacg tccaagagcg taccatcttc ttcaaggtaa
     9601 gtttaaacat atatatacta actactgatt atttaaattt tcaggacgac ggaaactaca
     9661 agacccgtgc cgaggtcaag ttcgagggag acaccctcgt caaccgtatc gagctcaagg
     9721 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag ggaatcgact
     9781 tcaaggagga cggaaacatc ctcggacaca agctcgagta caactacaac tcccacaacg
     9841 tctacatcat ggccgacaag caaaagaacg gaatcaaggt caacttcaag gtaagtttaa
     9901 acatgatttt actaactaac taatctgatt taaattttca gatccgtcac aacatcgagg
     9961 acggatccgt ccaactcgcc gaccactacc aacaaaacac cccaatcgga gacggaccag
    10021 tcctcctccc agacaaccac tacctctcca cccaatccgc cctctccaag gacccaaacg
    10081 agaagcgtga ccacatggtc ctcctcgagt tcgtcaccgc cgccggaatc acccacggaa
    10141 tggacgagct ctacaagtca ggagctagcg gagcctaccc ttacgacgtg ccagattacg
    10201 cttaccccta cgacgtacca gactacgcct acccatacga cgtcccagac tacgcttaag
    10261 ataaatgcaa aatcctttca agcattccct tcttctctat cactcttctt tctttttgtc
    10321 aaaaaattct ctcgctaatt tatttgcttt tttaatgtta ttattttatg actttttata
    10381 gtcactgaaa agtttgcatc tgagtgaagt gaatgctatc aaaatgtgat tctgtctgat
    10441 gtactttcac aatctctctt caattccatt ttgaagtgct ttaaacccga aaggttgaga
    10501 aaaatgcgag cgctcaaata tttgtattgt gttcgttgag tgacccaaca aaaagaggaa
    10561 actttattgt gccgccaaga aaaaagtctc acgtacggtg cgcgcgatgc cctaggcatg
    10621 gtcatagctg tttcctgcgc aagtcggcga tttctttgaa gttttgtcaa aagaagagac
    10681 aaggacttga tagatacgct catcagagtg agattcattg caaatagtgt gtaaatcgtg
    10741 gagagacgag agaaaaggtg tgagaagctt agaagataaa gaataggaag aagaacatat
    10801 ctcatttcct attttctcta atttttcgtt catttttcta gacattaccc atttgatggt
    10861 ttttcaatat ggttatcagg gtactcattt tggtagtttt caaagcaatt tggagttttt
    10921 ttttaaattg tcaactacaa aaacgcagct taaaggtcca aatatcatag tataaaaatt
    10981 ctttaaaaaa ttccaaattt tatatgattt tttgaaaatt ggaagaatgt tagtttttgc
    11041 catttttttg gaagtttttc tccggatttt atggtactta tatttcaaat ataaattttt
    11101 gtaggaattc gagtatgcga gagagctata gtttactaaa aaaaagtggc aaaaactcag
    11161 taattgccac ttttttcaaa aatgttttga aaaattttac tatgatattt ggccattttg
    11221 gcaccatatg tacgttactt tttacgaaac actctaattt ttgaaatttg agtttcatgt
    11281 ttaatctttt gtataaaatt tttatggtcc ttttgtgaca ggataaaaaa ttgcaaaaaa
    11341 atagaaaact ttgtttcgtg aaaacaccta gtttaaaaat tgtgggccac ttagcgattc
    11401 tagtcaaaaa tcagacaaat gtcagctgcc gtaacttttt ccggatcagg gttatcaaat
    11461 tttaatttta attgtaaatg tagaaaaatg atcggcaaca ttttgctaat tgaaaatatt
    11521 ttgaaaattg ctatagtaca tattaatcta gtattaatat agtattaatg ttataatatc
    11581 agttatgtgt ttgaataact caagcgtaag tttccgtaaa atatctttaa attttggtaa
    11641 ttttttacgg tttggtataa atttttgcat tttttgattt tctacacttt ttttcgattt
    11701 cgcattttcc gaatttctct gaaataaatc gaacaatcgg aaaattttcg agggcaccct
    11761 tagccatgca gctcaggaat ttgactttat gttcccattc aaattttttt aaaattaaat
    11821 aaaatgctaa ttgtaagata aattacaaaa attattaaat ctcatcatcc ttatgatgat
    11881 agtgcagtgc atgaatatat ttttccaaat aggatatgat tcgtcgagta gcttcacgta
    11941 atccgttttc ttcaatcacc atttcgaact gcaatttttt ttttggattt ttgtaacaat
    12001 attgtcataa attgtgattt tgatattttg cgtagagcat cagaaccttt ctgctttgaa
    12061 ttttaatggg attaaaaatt ataaaaaact tgcaccctaa ttaaagcgcc aactttatta
    12121 tacatagttg ataattcgat ggtatcagct cactcaaagg cggtaatacg gttatccaca
    12181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gaccaggaac
    12241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    12301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    12361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    12421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    12481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    12541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    12601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    12661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    12721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    12781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    12841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    13021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    13081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    13141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    13201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    13261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    13321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    13381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    13441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    13501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    13561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    13621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    13681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    14041 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    14101 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    14161 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    14221 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    14281 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    14341 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    14401 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggatccg ccaagctatc
    14461 aactttgtat agaaaagttg
//
