LOCUS       sequence-317226-        7919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0250:Dendra
FEATURES             Location/Qualifiers
     source          1..7919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3863..3886
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4143..4943
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      5068..5656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5557..5576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgttcgcctg gtggggtcgg accgtgtacc agttcaggta
      121 catcgtcatc ggtgtgatgg tggccctgtg tctcggcggc ggcgtctacg ggatcagcct
      181 gggtaaccac gtcacgcaga gcggtttcta cgacgagggc agccagtctg tcgcggcgtc
      241 gctgatcggc gacgaggtgt acggccgtga ccggaccagc cacgtggtgg ccatcctgac
      301 gcctcccgac gacaagaagg tcaccgacaa ggcgtggcag aagaaggtca ccgaggaact
      361 cgatcaggtg gtcaaggacc acgaggatca gatcgtcggc tgggtgggct ggctcaaggc
      421 ccccgacacc accgacccga ccgtcagcgc catgaagacg caggatctgc ggcacacgtt
      481 catcagcatc ccgctccaag gtgacgacga cgacgagatc ctcaagaact atcaggtcgt
      541 cgagcccgag ctgcagcagg tcaacggcgg cgacatccgg ctcgcgggcc tcaacccgct
      601 ggccagtgag ctgaccggca ccatcggcga ggaccagaag cgcgccgagg tggccgccat
      661 cccgctggtg gccgtggtgc tgttcttcgt gttcggcacc gtgatcgccg cggccctgcc
      721 cgcgatcatc ggtggcctcg ccatcgcggg cgcgctgggc atcatgcgcc tggtcgccga
      781 gttcaccccc gtgcacttct tcgcccagcc ggtggtcacg ctgatcggcc tcggtatcgc
      841 gatcgactac ggcctgttca tcgtgagccg gttccgcgag gagatcgccg agggctacga
      901 caccgaggcg gccgttcgcc gcaccgtgat gacgtcggga cgcacggtcg tgttctccgc
      961 ggtgatcatc gtcgcgtcgt cggtgccgct gctgctgttc ccgcagggct tcctgaaatc
     1021 gatcacctac gccatcatcg cgtcggtcat gctcgcggcg atcctgtcga tcaccgtgct
     1081 ggcggccgcg ctggcgatcc tgggcccgcg agtcgacgcg ctgggcgtga cgacgctgct
     1141 gaagatcccg ttcctggcca actggcagtt ctcgcggcgc atcatcgact ggttcgccga
     1201 gaagacgcag aagaccaaga cgcgcgaaga ggtcgagcgc ggcttctggg gccggctggt
     1261 caacgtcgtg atgaagcgcc cgatcgcgtt cgccgcgccg atcctggtgg tgatggtgct
     1321 gctgatcatc ccgttggggc agctgtccct cggcggtatc agcgagaaat acctgccgcc
     1381 ggacaacgcc gtgcgccagt cgcaggagca attcgacaag ctgttccccg gtttccgtac
     1441 cgaaccgctc acgctcgtga tgaagcgtga ggacggcgag ccgatcaccg acgcccagat
     1501 cgccgacatg cgcgccaagg cgctgacggt ctccggcttc accgacccgg acaacgatcc
     1561 ggagaagatg tggaaggaac gcccggccaa cgacagcggg tccaaggacc cgtcggtgcg
     1621 ggtgatccag aacggtctgg agaaccgcaa cgacgcggcc aagaagatcg acgagttgcg
     1681 cgcgctgcaa ccaccgcacg gcatcgaggt gttcgtcggc ggaaccccgg cgcttgaaca
     1741 ggactcgatc cacagcctgt tcgacaagct gccgctgatg gcgctgatcc tgatcgtcac
     1801 gacgaccgtc ctgatgttcc tggcgttcgg atcggtggtg ttgccgatca aggccgcgtt
     1861 gatgagcgcg ctgacgctcg gttcgacgat gggcatcctc acgtggatgt tcgtcgacgg
     1921 ccacggctcg gggctgatga actacacccc gcagccgctc atggcgccca tgatcggcct
     1981 gatcatcgcg gtcatctggg gtctgtcgac cgactacgag gtgttcctgg tgtcccgcat
     2041 ggtcgaggca cgtgaacgcg gtatgtcgac ggccgaggcc atccgcatcg gtacggcgac
     2101 cacgggccgc ctgatcaccg gcgcggcgct gatcctcgcg gtggtcgcgg gtgcgttcgt
     2161 gttctccgac ctggtgatga tgaagtacct ggccttcggt ctgctcatcg cacttctgtt
     2221 ggacgccacc atcattcgta tgttcctggt gcccgcggtg atgaagctgc ttggcgacga
     2281 ctgctggtgg gccccgcgct ggatgaagcg ggtgcaggag aagctgggtc tcggcgagac
     2341 cgaactgccc gacgagcgca agcgtcccac cgtgcgcgag tccgagaccg atcagcgggc
     2401 cctggtgggc gtcggcgcac cgccgccgcc accgcgtccg cacgatccga cgcatcccgc
     2461 accggaaccg gtccgcccga tgccgccgat gcgcagcaac gcgccgtcgg ccgcgggtac
     2521 cgcgcgcatc tcgacaccgc ctcagccgcc tcagccgccg caggcgcccg ctcagcaggc
     2581 cggcgacgaa cccgccacca cgcggttcgc catggcgcgc aacgccgttc gcaatgcggt
     2641 caacagcgcg gtgcacggcg gcgcaggcag cgcggccgcg ccgaccgagc gtgcgccgcg
     2701 cccgggtggc ccggcgcagc cgccggcccc gccgcagcgc gaggaacgcg agatcgagtc
     2761 ctggctgggc gcgttgcgtg gccccgcgcc ggccaagaac gtgccccagc cgccggccca
     2821 gccgcagcgg ccgtcgacgg acaccacgcg tgccatgccc ccgcagggcc gcccgcccgc
     2881 cgggcccgcc gaccggggca acgagaacgc gcccaccacg gcgttcagcg cgcagcgtcc
     2941 gcccaacggc ggtgcgcccg cggatgcgac gacggcgatc cccacgcccc cgcagcggga
     3001 gcaggaaccg tcgaccgaga agctcaacac gcgtgaggac gctccggagg atccggagac
     3061 caagcgccgt ggcggcggca tgagcgcgca ggacctgctg cgccgcgaag gcaggctgaa
     3121 gcttggtgcg gccgcgggca acacgcctgg catcaatctg atcaaggagg acatgcgtgt
     3181 caaggtgcat atggagggca acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa
     3241 gggtaagcct tacgaaggga cccagaccgc caacctcacc gtcaaggagg gcgcccctct
     3301 cccattctcg tacgatatcc tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa
     3361 gtaccccgag gatatccctg actacttcaa gcagagcttc ccggagggat actcctggga
     3421 gcggacgatg accttcgaag acaagggtat ctgtacgatc cgcagcgaca tctcgctgga
     3481 aggggactgt ttcttccaga acgtccgttt caaggggacc aactttccgc cgaacggccc
     3541 ggtcatgcag aagaagaccc tcaagtggga gccgagcacg gagaagctgc acgttcggga
     3601 cggcctcctg gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg gccactacct
     3661 atgcgacttc aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt
     3721 cgtggaccac cgcatcgaaa tcctggggaa cgattcggac tacaacaagg tcaagttgta
     3781 cgagcacgct gtggcacgct atagcccgct cccgtcgcag gtctggttcg aaggcggcgg
     3841 cggggagaac ctgtatttcc aggactacaa ggacgacgac gacaagtgat ttaaataacc
     3901 ccttggggcc tctaaacggg tcttgagggg ttttttgtta actagcgtac gatcgaattc
     3961 ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat
     4021 gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg
     4081 atgataagtt tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac
     4141 tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca
     4201 tcggtcagct tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct
     4261 tccgtagcgt ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgcta
     4321 cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt
     4381 tcgatcctgc cacgtcgccc gttacaccgg accttggagt tgtctctgac acattctggc
     4441 gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg
     4501 cagagcagat catctctgat ccattgcccc tgccacctta ctcgcctgca agcccggtcg
     4561 cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca
     4621 cgacgctgca tcttgccgag ttgatggcaa aggttcccta tggggtgccg agacactgca
     4681 ccattcttca ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg
     4741 agcgctttgc cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag
     4801 tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc
     4861 aactgggccg agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg
     4921 atgccgctcg ccagtcgatt ggctgagctc atgagcggag aacgagatga cgttggaggg
     4981 gcaaggtcgc gctgattgct ggggcaacac gggggatcca ctagttccat gagcgtcaga
     5041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5461 atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg taagcggcag
     5521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5701 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5761 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg
     5821 ccgcggtacc cggggatcct ctagagtcga ccaccaaggg caccatctct gcttgggcca
     5881 ccccgttggc cgcagccagc tcgctgagag ccgtgaacga cagggcgaac gccagcccgc
     5941 cgacggcgag ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg tctattctct
     6001 tcactgcacc agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc
     6061 tctattcgtt tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc
     6121 ccaggtcaga agagtcgcac aagagttgca gacccctgga aagaaaaatg gccagagggc
     6181 gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg aacccgcgta gctagtttgg
     6241 aagaatgggt gtctgccgac cacatatggg ccggtcaaga taggttttta ccccctctcg
     6301 gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt
     6361 tgcacaggag ttgcaacccg gtagccttgt tcacgacgag aggagaccta gttggcacgt
     6421 cgcggatggg gatcgctgaa gactcagcgc agcgggagga tccaagcctc atacgtcaac
     6481 ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg acaacaagat ggacgccgaa
     6541 gcctggctcg cgggcgagaa gcggctcatc gagatggaga cctggacccc tccacaggac
     6601 cgggcgaaga aggcagccgc cagcgccatc acgctggagg agtacacccg gaagtggctc
     6661 gtggagcgcg acctcgcaga cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc
     6721 atctacccgg tgctaggtga agtggcggtc acagagatga cgccagctct ggtgcgtgcg
     6781 tggtgggccg ggatgggtag gaagcacccg actgcccgcc ggcatgccta caacgtcctc
     6841 cgggcggtga tgaacacagc ggtcgaggac aagctgatcg cagagaaccc gtgccggatc
     6901 gagcagaagg cagccgatga gcgcgacgta gaggcgctga cgcctgagga gctggacatc
     6961 gtcgccgctg agatcttcga gcactaccgg atcgcggcat acatcctggc gtggacgagc
     7021 ctccggttcg gagagctgat cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg
     7081 atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac
     7141 gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc
     7201 cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc ccgaggcatt cctggtgacc
     7261 acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac
     7321 gccaagatcg gtcggccgga actccgcatc cacgacctcc gcgctgtcgg cgctacgttc
     7381 gccgctcagg caggtgcgac gaccaaggag ctgatggccc gtctcggtca cacgactcct
     7441 aggatggcga tgaagtacca gatggcgtct gaggcccgcg acgaggctat cgctgaggcg
     7501 atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag
     7561 tcctaaagag gggggtttct tgtcagtacg cgaagaacca cgcctggccg cgagcgccag
     7621 caccgccgct ctgtgcggag acctgggcac cagccccgcc gccgccagga gcattgccgt
     7681 tcccgccaga aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat
     7741 attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac gtgatgatcg
     7801 gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg atctgtgcgt
     7861 tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaact ccctatcag
//
