LOCUS       sequence-317227-        5246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0245:Dendra
FEATURES             Location/Qualifiers
     source          1..5246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1190..1213
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1470..2270
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2395..2983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2884..2903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcgagcgac tagcgcccgc gccctgctgg ccgatctggt
      121 ctgtgtggtg gtgttctgca cgatcggcag gcgcagccac gccgagggga tcaccgtggc
      181 cggcgtcgcc gagacggcct ggccgtttct ggtcggcacg ctcgtcggct gggtggtctc
      241 gcgcggctgg cagcgcccga catcgttggc gcccaccggc atcgcggtgt gggcgtgcac
      301 catcgtcgtc ggcatgctgc tgcgcaagct cacctcggcc ggcaccgccg tcagcttcat
      361 cgtggtggcc tcgctggtca ccgcggcact tctgctcggc tggcgcggcg tgctggccgc
      421 cgcgcgacgc aaggctcagg ccaacaagct tggtgcggcc gcgggcaaca cgcctggcat
      481 caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca
      541 cgccttcgtg atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa
      601 cctcaccgtc aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt
      661 gcattacggg aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca
      721 gagcttcccg gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg
      781 tacgatccgc agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa
      841 ggggaccaac tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc
      901 gagcacggag aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct
      961 gctgctggaa ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa
     1021 ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga
     1081 ttcggactac aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc
     1141 gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga
     1201 cgacgacgac aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt
     1261 tttgttaact agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca
     1321 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     1381 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     1441 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     1501 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     1561 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     1621 ttggactgat cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct
     1681 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     1741 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     1801 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     1861 caccttactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     1921 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     1981 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     2041 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     2101 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     2161 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     2221 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     2281 agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg
     2341 ggatccacta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2401 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2461 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2521 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2581 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2641 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2701 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2761 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     2821 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2881 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2941 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3001 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3061 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3121 ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca
     3181 ccaagggcac catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg
     3241 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     3301 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     3361 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     3421 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     3481 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     3541 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg
     3601 gtcaagatag gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt
     3661 cgtagaagcg cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca
     3721 cgacgagagg agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc
     3781 gggaggatcc aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag
     3841 acctacgaca acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag
     3901 atggagacct ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg
     3961 ctggaggagt acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat
     4021 ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca
     4081 gagatgacgc cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact
     4141 gcccgccggc atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag
     4201 ctgatcgcag agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag
     4261 gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc
     4321 gcggcataca tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc
     4381 aaggacatcg tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc
     4441 gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg
     4501 gttccgcctc acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac
     4561 aagggccccg aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg
     4621 ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac
     4681 gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg
     4741 atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag
     4801 gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca
     4861 aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga
     4921 agaaccacgc ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag
     4981 ccccgccgcc gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc
     5041 gcccgctttg atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag
     5101 gcggcacgat ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct
     5161 acgccatccc ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc
     5221 tgacgagcgg gagaactccc tatcag
//
