LOCUS       sequence-317231-       12828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA,
            and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI
            sites for spacer cloning. pCDF backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH
  TITLE     CRISPR RNA-guided integrases for high-efficiency, multiplexed 
            bacterial genome engineering.
  JOURNAL   Nat Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y.
  PUBMED    33230293
REFERENCE   2  (bases 1 to 12828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y."
COMMENT     Sequence Label: pSL1425 (pSPIN, pCDF backbone)
FEATURES             Location/Qualifiers
     source          1..12828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..739)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(949..2031)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1993..2012
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2032..2109)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2218..2235
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2246..2280
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             9262..9273
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10708..10726)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10722..10769
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             10924..11583
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10990..11009)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(11783..11800)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11783..11799)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11792..11814)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(11898..12689)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(12690..12781)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg
       61 tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca
      121 gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg
      181 aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt
      241 cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt
      301 tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt
      361 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
      421 cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact
      481 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
      541 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
      601 tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc
      661 acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
      721 tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc aatttatctc
      781 ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc
      841 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
      901 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
      961 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1021 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     1081 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     1141 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     1201 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
     1261 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     1321 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     1381 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     1441 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     1501 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     1561 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     1621 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     1681 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     1741 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     1801 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     1861 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1921 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1981 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     2041 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     2101 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aatggataac
     2161 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2221 gagtcagtga gcgaggacac aagctttgac agctagctca gtcctaggta taatactagc
     2281 gtcgacgtgg agatatacca tgggtgaact gccgagtagg tagctgataa cgagacctct
     2341 gtcttgtcag ctagggtggt ctcgtgaact gccgagtagg tagctgataa cggatccgaa
     2401 ttcgagcgaa ggagatatac atatgttttt gcaaagacct aaaccttaca gcgatgaaag
     2461 tttagaaagt ttctttatcc gagtggctaa caaaaatggc tacggtgatg tccatcgctt
     2521 cctagaagcc actaaacgat tccttcaaga cattgaccat aatggctatc aaacctttcc
     2581 gactgatata actcggataa acccatactc agctaaaaac agttccagcg cacgaactgc
     2641 gtcattcctg aaacttgcac aattgacatt taatgaaccg ccagagctac ttgggttggc
     2701 aattaacaga acaaacatga aatactcgcc gtcaactagc gcggttgtta gaggtgcaga
     2761 agtctttcct cgcagtttac tacggacgca ctccatcccc tgctgtcctt tgtgtctgcg
     2821 agaaaatggc tacgcctcct acctttggca ctttcagggg tacgaatact gccacagcca
     2881 taacgtacct ttaattacca cttgtagctg tggtaaggag tttgactacc gagtatctgg
     2941 gttaaagggc atttgctgca aatgcaagga gcctatcacc ttaaccagca gggagaacgg
     3001 tcatgaggca gcgtgtactg tttcaaactg gcttgctggc catgaatcta aacctctgcc
     3061 aaatcttcct aaaagctacc gatggggttt agttcattgg tggatgggta ttaaagatag
     3121 cgagttcgat cacttttcgt tcgttcaatt tttctcaaac tggccaaggt cattccactc
     3181 gataatcgaa gatgaagtag agttcaacct tgagcatgct gttgtcagca cgtctgaatt
     3241 acgactaaaa gatcttcttg gtcgattgtt tttcggttca attcggttac ctgagcggaa
     3301 tcttcaacac aatatcatcc ttggtgagct tctctgctat ttagaaaatc gtttatggca
     3361 agacaaggga ttaatcgcca acctcaaaat gaacgcgtta gaggcgactg taatgttaaa
     3421 ttgtagcctc gatcagattg catcaatggt tgaacaacgc atcttgaagc caaatcgaaa
     3481 aagcaagccc aacagccctc ttgatgttac cgattatcta tttcatttcg gcgatatttt
     3541 ctgtctttgg ttagctgagt tccaaagcga tgagtttaac cgttcgtttt atgtgtcgag
     3601 gtggtaaatg caaactctga aagaactaat cgcatccaat cctgacgact taacaactga
     3661 acttaagaga gcatttcgtc cactcacacc gcatattgca attgatggta atgaacttga
     3721 cgcactgacg atattagtca atttaaccga taagactgat gatcagaaag acctgctcga
     3781 tcgagccaaa tgcaagcaaa aacttcgaga tgaaaaatgg tgggctagct gcataaattg
     3841 cgttaattac agacaaagcc ataacccaaa attcccggat atacgttctg aaggcgtgat
     3901 tcgaacccaa gccctgggtg aattaccgag tttcctactc tcgtcctcaa aaatcccacc
     3961 ataccattgg tcatatagcc atgattcaaa atacgtcaac aaaagcgcat tcctcaccaa
     4021 tgagttttgt tgggatggtg agatctcatg tttgggtgag cttcttaaag atgcagatca
     4081 cccactttgg aatactttga aaaagttagg ttgttctcaa aaaacttgca aagcaatggc
     4141 aaaacaacta gctgatatta ctctcacgac tatcaatgtc acactcgcac caaattacct
     4201 gactcaaata tctctccccg atagtgatac atcctacatt tcactctctc ctgttgcatc
     4261 gctatcgatg caaagccact ttcatcagag gctccaagat gaaaatcggc atagcgcgat
     4321 aacgcggttt agccgaacta ccaacatggg ggtcactgcg atgacatgtg gcggcgcatt
     4381 tagaatgtta aagtctggcg ctaagttttc cagcccccct catcaccgat taaacagtaa
     4441 acgaagttgg ttgacgtcag agcatgttca gtcattaaaa cagtaccagc gcctcaacaa
     4501 aagcctcata cctgaaaact ctcggattgc actccgtaga aaatacaaaa tcgagcttca
     4561 aaatatggtc agatcttggt ttgcaatgca agaccatacc cttgattcga acatacttat
     4621 ccaacatttg aatcatgacc tatcttactt aggagccaca aaacgttttg catacgatcc
     4681 agcgatgacc aagctcttta ctgagctttt gaaacgagag ttatcaaatt caatcaataa
     4741 tggtgagcaa cacactaatg gatcgttttt agtcctaccg aatatcaggg tttgtggcgc
     4801 aacagcttta agctccccgg taacggtggg gattccatca cttacagctt tctttggctt
     4861 cgttcacgca tttgaacgga atataaatcg caccacctca tcgtttcgtg ttgaatcctt
     4921 tgcgatatgc gtccatcaac tacatgtcga gaagcgaggt ttgacagcag agtttgtgga
     4981 aaaaggcgac gggactatat ccgctcccgc gacccgggat gactggcagt gtgatgtcgt
     5041 atttagcctt attttgaaca ccaactttgc tcaacatatt gaccaagata cgttagttac
     5101 atcactacca aagcgattgg ctcggggttc agcaaaaatt gcgattgatg actttaaaca
     5161 tatcaactca ttctcgacat tagaaacagc gatcgaatct ctgccaatag aagctggtag
     5221 gtggttatca ctttacgcac agtcaaacaa taatctaagt gatctattag cagccatgac
     5281 agaggaccat cagctcatgg caagctgcgt cggttaccac ttgttagaag agcccaaaga
     5341 taaaccaaac tccctcagag gttacaaaca cgctatcgcc gagtgcatca ttggactcat
     5401 taactcaatc acctttagct cagagactga tcccaacaca atcttttggt cgctaaagaa
     5461 ctatcaaaac tacctagtgg tacagccaag gagtatcaac gatgaaacta ccgacaaatc
     5521 tagcctatga gcgctctatc gacccatcag atgtctgttt ttttgtcgtc tggcccgatg
     5581 atagaaaaac acctttaacc tacaattctc gtactctgct cgggcaaatg gaagcggcat
     5641 cattagccta tgatgtctca ggtcaaccaa taaaaagtgc caccgctgag gcgttagctc
     5701 aagggaaccc tcatcaagtt gatttctgcc acgttccata cggtgcgagt catattgaat
     5761 gcagtttctc cgtctcgttt tcttctgaac tacgtcaacc atataagtgt aactcaagca
     5821 aagttaaaca aacgctagtg caattagtcg agctctacga aacgaaaatc ggctggactg
     5881 agctagcaac ccgatatttg atgaacattt gcaacggtaa atggctgtgg aaaaataccc
     5941 gtaaagccta ttgctggaac attgtactta caccttggcc ttggaacggg gaaaaggttg
     6001 gatttgaaga tattcgtact aactacacct cacggcaaga ctttaaaaat aataaaaatt
     6061 ggtctgctat agttgaaatg atcaaaaccg cattttctag tactgatggg ctggcgatat
     6121 ttgaagtcag ggccaccttg cacttgccaa cgaatgctat ggtgcggcca agccaagttt
     6181 tcacagaaaa agaaagtggc agtaaaagta aatctaaaac tcaaaacagt cgagtttttc
     6241 agagtacaac tattgatggt gaacgatcgc caatactagg ggcctttaaa acgggagcag
     6301 ctattgcaac cattgacgac tggtatcctg aagccactga gccactaagg gtcggacggt
     6361 ttggggttca tcgcgaagat gtcacttgct accgtcatcc gtctaccgga aaagattttt
     6421 tctcgatatt acaacaagca gagcactata ttgaagtgtt gagcgccaac aaaactcccg
     6481 ctcaagaaac tatcaacgac atgcactttt taatggctaa cctgattaag ggtgggatgt
     6541 tccagcataa aggagactga ctgtgaaatg gtattataag acaatcacct ttctgccaga
     6601 gttgtgcaac aacgagtcac tggctgcaaa gtgtctccgc gttctgcatg gatttaacta
     6661 tcagtatgag acacgaaata tcggcgtttc atttccgctt tggtgtgatg caacggttgg
     6721 aaaaaagatt tcatttgtca gcaagaacaa gatagaactc gacttactac ttaaacaaca
     6781 ctatttcgtc caaatggaac aacttcaata ctttcatata tccaacactg ttctcgtccc
     6841 agaagattgt acatacgttt cctttagacg ctgtcaatct atagataagc tcacagcagc
     6901 agggctggca aggaaaatca gacgcctgga gaaacgtgct ctatctagag gcgagcaatt
     6961 tgacccatca tcttttgctc aaaaagagca tactgcaata gcgcactacc actcacttgg
     7021 ggagtccagc aaacagacga accgcaactt tcgactcaat atcaggatgc tctcggagca
     7081 accccgtgag gggaactcga tttttagtag ctatggctta tcaaattcag aaaactcgtt
     7141 tcagcctgta cccttaatct gacctttaat aaggagatat accatggcga caagtttacc
     7201 tacgccctca gcaattacga cttcggcgtt agagtatgca ttccatactc ccgctcgcaa
     7261 tctaacgaaa tctcgcggaa aaaacattca tcgttatgtc agtgtaaaga tgagtaagag
     7321 gattacggta gaatctactc tagagtgtga tgcctgctat cactttgatt ttgagccaag
     7381 tattgttcgc ttttgcgctc aaccgattcg atttttatat tatctcaatg gtcagtctca
     7441 ctcctatgtt cctgactttc tagttcaatt tgataccaac gagtttgttc tatatgaagt
     7501 aaagtcagct tatgctaaga acaaacctga ttttgatgtt gaatgggagg cgaaagtaaa
     7561 agcagcaact gaactagggc tagaattgga gcttgttgaa gagagtgata ttagggatac
     7621 ggttgtatta aataatctta agcgcatgca tcgttatgct tcgaaagatg agctgaataa
     7681 cgtacataac tctctcttaa aaataataaa gtacaatggc gcccaatctg caagatgctt
     7741 gggagaacag ttgggtttaa aaggccgaac tgttttacca attttgtgcg atttgctgtc
     7801 aaggtgttta ctcgatacac gtttggataa gcctctatct cttgaatctc gatttgagtt
     7861 ggccagttat ggctaagaaa gggttctcaa gtttccatag aaaagcagtc tcttcccaag
     7921 atacgcttga atcgatagag cttgtctcta gcgctaattg tctagaaagt gttacgtatc
     7981 aagatatatc agcatttccc gaaacaattg cggtagagat taatttccga ttaagcattc
     8041 ttcgcttttt agcgcgaaag tgcgaaacca ttgtggccaa atcaattgaa ccacatcgtg
     8101 tagagctaca gcaaaactat agtagaaaaa tacccagtgc aataacgata tatcgatggt
     8161 ggcttgcttt tcgaaaatca gactacaacc ccattagctt agcacctaat atcaaggata
     8221 gaggtaatag agagacaaaa gtgtcaacag ttgttgattc tattatggaa caggcagttg
     8281 aaagagttat atctggacga aaagtcaatg ttagctctgc atataaacgt gttcgacgaa
     8341 aagttcgtca atacaatcta actcatggaa cgaaatacac gtatcctaag tacgaatctg
     8401 taagaaagcg agtaaaaaag aaaaccccat ttgagttatt agccgcaggg aaaggggaga
     8461 gagtagctaa gagagagttt cgccgaatgg gaaaaaagat cctcacgtct agcgtgctag
     8521 agagggttga aatagatcac actgtcgttg acctttttgc agtacacgaa gagtatcgaa
     8581 tcccattggg ccgaccttgg cttactcaat tggttgattg ttacagtaaa gctgtaatcg
     8641 gtttttattt aggtttcgag cctcctagct atgtgtcggt ttcccttgca cttaagaatg
     8701 caatacaacg caaagatgac ttaatctcct cgtatgaatc gatcgagaat gaatggctat
     8761 gttatggcat cccagaccta ctcgtaactg ataatggtaa agagtttttg tcgaaagcat
     8821 ttgatcaagc atgtgaatca ctattgatca atgtgcatca aaataaagtt gagacgcccg
     8881 acaacaaacc tcatgttgaa cgtaactacg ggactattaa tacttctctg ttagacgatt
     8941 tacctgggaa atccttcagc cagtaccttc aaagagaagg gtacgactct gtgggagaag
     9001 ctacccttac actcaatgag attagagaaa tttacttaat ttggttggtg gatatttatc
     9061 ataaaaaacc caatcagaga ggcactaatt gtcctaatgt tgcgtggaaa aagggttgtc
     9121 aagaatggga accagaggag ttctctggtt ctaaagacga attagacttt aaatttgcta
     9181 ttgttgatta caaacaactt actaaagtag ggataactgt ctacaaagaa ctgagttata
     9241 gcaatgaccg tttagctgaa tatagaggga agaaaggaaa ccataaagtt cagttcaagt
     9301 ataaccctga gtgtatggca gttatttggg tgttggatga ggatatgaat gagtacttta
     9361 cagttaatgc gattgactac gaatatgcaa gtagagtatc actttggcaa cataaatata
     9421 acatgaaata tcaagcagaa ctaaattcag cagaatatga tgaggacaag gaaattgatg
     9481 cagaaataaa aattgaagaa atcgcagatc gttcaattgt taagactaac aaaatcagag
     9541 ctcggaggcg tggcgctagg catcaagaga atagcgcaag ggctaagtca atcagtaatg
     9601 cgaacccggc ctcgatacaa aaacatgaag atgaaatcgt tagtgcagat aatgacgatt
     9661 gggatattga ttatgtctga gaaatcgtca aatgagtgaa acgcgtgagg ctcgtatatc
     9721 aagagctaaa agggcatttg tatccacacc gagcgttagg aaaatcttga gttacatgga
     9781 tagatgtaga gatctatcag acctagagtc tgagcctaca tgcatgatgg tctatggtgc
     9841 ttcgggtgta ggtaaaacga ccgtcatcaa gaaatactta aatcaaaaca gaagagagtc
     9901 cgaagccggg ggcgatataa taccggtttt gcatattgag ttgccagaca atgcgaagcc
     9961 agtagatgca gcaagggaat tgctggttga aatgggtgac ccgctagcac tttatgaaac
    10021 tgacttagct agattgacga aaagactgac tgaattaatc cctgcggtcg gcgtgaagct
    10081 gattattatc gatgagttcc aacatttggt ggaagaaagg tcaaatcggg ttcttaccca
    10141 agtaggtaat tggctaaaaa tgatacttaa caaaacgaaa tgtccaattg ttatatttgg
    10201 tatgccatac tcaaaagttg tactgcaagc aaactcgcaa cttcacgggc gattttccat
    10261 tcaggttgaa ctgcgcccct ttagctacca gggaggtaga ggtgtattta aaactttttt
    10321 ggaatacctt gataaagccc taccttttga aaaacaggct ggcttagcca acgaaagttt
    10381 gcagaaaaaa ttgtatgcat tctctcaggg aaacatgcgt tcgttgagaa accttattta
    10441 tcaagcatct atcgaagcaa ttgataatca gcatgagacg ataaccgaag aagatttcgt
    10501 ttttgcatcg aagttgacat cgggcgataa acccaactca tggaaaaatc cttttgagga
    10561 gggtgttgag gtaacagaag atatgttacg accgccacca aaagatattg gttgggagga
    10621 ctatttgaga cattcaaccc cgagagtgag taaaccaggt agaaataaaa actttttcga
    10681 ataacctagg ctgctgcgct gctgccaccg ctgagcaata actagcataa ccccttgggg
    10741 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgtt gttgatacaa
    10801 ccataaaatg ataattacac ccataaattg ataattatca cacccataaa ttgatattgc
    10861 ctcttcatgg tctaaacttc agtaagttta cgacattttc ctcgaggtca tttccgggga
    10921 tccatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
    10981 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
    11041 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
    11101 attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc aatgaaagac
    11161 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
    11221 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
    11281 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
    11341 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
    11401 gtggccaata tggacaactt cttcgccccc gttttcacta tgggcaaata ttatacgcaa
    11461 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
    11521 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
    11581 taattttttt aaggcagtta ttggtgccct tctagagtcc ttactgcagt agttttgctg
    11641 aaatactcga ttcacaaaaa tatcaactta tggttgtttt gtgagatatc aatatatggt
    11701 tgttttgtgg ttaagttgct gattataaat aattattaaa tatcacttta tggttgcatc
    11761 aacaggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    11821 ctggcgttac ccaacttaat cgccttgcta tcgtggccgg atcttgcggc ccctcggctt
    11881 gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
    11941 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
    12001 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
    12061 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
    12121 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
    12181 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
    12241 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
    12301 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
    12361 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
    12421 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
    12481 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
    12541 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
    12601 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
    12661 gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt
    12721 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    12781 atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgg
//
