LOCUS       sequence-317236-        1581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GPT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC2-GPT1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..1581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1510..1575
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggcgagca gcacaggaga tcgaagtcag gcagttcgac acggtttgcg cgccaaagtt
       61 ttgacgctgg atggcatgaa tccgagggtg cgccgcgtgg aatatgcagt gcgaggaccc
      121 attgtgcaga gagctcttga acttgagcag gagctgcgcc agggggtgaa aaagccgttt
      181 actgaggtta tacgggcgaa catcggagac gctcaagcga tgggccaacg acccattaca
      241 tttcttcggc aggtgttggc gctttgcgta aatccggatt tgttgtcttc ccccaatttc
      301 cctgatgatg cgaaaaagag ggccgagaga atcctccaag catgcggtgg gcattcactt
      361 ggcgcatact ctgtctccag tggaatacaa ctgattcgcg aagacgttgc ccgctacata
      421 gagcgccgcg acggagggat ccctgctgat ccgaataacg ttttcttgag taccggagct
      481 agcgatgcta ttgtaactgt acttaaattg ttggtggccg gtgagggaca cacgagaacg
      541 ggagttctga tcccgatccc ccaatatcct ctttatagcg cgacgttggc tgagctcggt
      601 gcggtgcaag tagactatta tttggacgag gaacgagcct gggccttgga tgtggcagag
      661 ctccatcggg ccttggggca agcccgagat cattgtcgcc ccagagccct ttgcgtgatc
      721 aatccaggta acccgaccgg tcaggtacag actagggagt gtattgaggc agtaatcagg
      781 tttgcttttg aggagcgcct gtttttgctt gcggatgagg tttatcaaga taatgtgtac
      841 gcggctggat cacagtttca cagttttaaa aaggtactta tggagatggg tccaccttac
      901 gcgggtcaac aagagcttgc cagttttcac agcacgtcta aaggatatat gggtgaatgt
      961 ggattcagag ggggatatgt tgaagttgta aacatggatg ccgcagtgca acaacagatg
     1021 ttgaaactga tgagtgttcg gctgtgccca cccgtaccag gtcaagcgct tctggacttg
     1081 gtcgtttccc cccctgcacc cacagaccct agcttcgcac aattccaagc cgaaaaacaa
     1141 gctgtcctcg cggaactggc agcgaaggca aagctcacag aacaggtatt caacgaggcc
     1201 ccaggcatca gttgtaatcc tgtgcaaggc gcaatgtact ctttcccgag ggtccagctc
     1261 ccccctagag ctgtggaacg agcgcaggag ctcggcttgg cacccgacat gttcttctgt
     1321 ttgcggcttc tggaggagac gggtatttgc gttgtaccag ggagcggctt tgggcaacga
     1381 gaagggacgt atcattttcg catgacaata ctcccgccgc tcgaaaaact cagactcctt
     1441 ttggagaagc tgtctcggtt ccatgctaaa ttcacgttgg aatactccgg aggcagcgcg
     1501 gccgcaggcg actacaaaga ccatgacggt gattataaag atcatgacat tgattacaag
     1561 gatgacgatg acaagtaata a
//
