LOCUS       sequence-317238-        1662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GPT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC2-GPT2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..1662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1147..1156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1591..1656
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgcagcggg cggcagcact ggtcaggcga ggctgtggtc ctcggactcc aagctcctgg
       61 ggcagaagcc agagtagcgc tgctgccgaa gcctcggcgg tgctcaaggt gcggcccgag
      121 cgcagccggc gcgagcgcat cctcacgctg gagtccatga acccgcaggt gaaggcggtg
      181 gagtacgccg tgcggggacc catcgtgctc aaggccggcg agatcgagct cgagctgcag
      241 cggggtatca aaaagccatt cacagaggtc atccgagcca acatcgggga cgcccaggct
      301 atggggcagc agccaatcac cttcctccgg caggtgatgg cactatgcac ctacccaaac
      361 ctgctggaca gccccagctt cccagaagat gctaagaaac gtgcccggcg gatcctgcag
      421 gcttgtggcg ggaacagcct ggggtcctac agtgctagcc agggtgtcaa ctgcatccgt
      481 gaagatgtgg ctgcctacat caccaggagg gatggcggtg tgcctgcgga ccccgacaac
      541 atctacctga ccacgggagc tagtgacggc atttctacga tcctgaagat cctcgtctcc
      601 gggggcggca agtcacggac aggtgtgatg atccccatcc cacaatatcc cctctattca
      661 gctgtcatct ctgagctcga cgccatccag gtgaattact acctggacga ggagaactgc
      721 tgggcgctga atgtgaatga gctccggcgg gcggtgcagg aggccaaaga ccactgtgat
      781 cctaaggtgc tctgcataat caaccctggg aaccccacag gccaggtaca aagcagaaag
      841 tgcatagaag atgtgatcca ctttgcctgg gaagagaagc tctttctcct ggctgatgag
      901 gtgtaccagg acaacgtgta ctctccagat tgcagattcc actccttcaa gaaggtgctg
      961 tacgagatgg ggcccgagta ctccagcaac gtggagctcg cctccttcca ctccacctcc
     1021 aagggctaca tgggcgagtg tggttacaga ggaggctaca tggaggtgat caacctgcac
     1081 cctgagatca agggccagct ggtgaagctg ctgtcggtgc gcctgtgccc cccagtgtct
     1141 gggcaggccg ccatggacat tgtcgtgaac cccccggtgg caggagagga gtcctttgag
     1201 caattcagcc gagagaagga gtcggtcctg ggtaatctgg ccaaaaaagc aaagctgacg
     1261 gaagacctgt ttaaccaagt cccaggaatt cactgcaacc ccttgcaggg ggccatgtac
     1321 gccttccctc ggatcttcat tcctgccaaa gctgtggagg ctgctcaggc ccatcaaatg
     1381 gctccagaca tgttctactg catgaagctc ctggaggaga ctggcatctg tgtcgtgccc
     1441 ggcagtggct ttgggcagag ggaaggcact taccacttca ggatgactat cctccctcca
     1501 gtggagaagc tgaaaacggt gctgcagaag gtgaaagact tccacatcaa cttcctggag
     1561 aagtacgcgg gaggcagcgc ggccgcaggc gactacaaag accatgacgg tgattataaa
     1621 gatcatgaca ttgattacaa ggatgacgat gacaagtaat aa
//
