LOCUS       sequence-317240-        2100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-GLS-3xFLAG
FEATURES             Location/Qualifiers
     source          1..2100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2029..2094
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgatgcgcc tgaggggatc aggtatgctc agagatcttc tccttcggag tcctgcgggc
       61 gtgagcgcta ctttgcgacg ggctcaaccg ctggttacac tctgtcggcg acccaggggc
      121 ggcgggcgac cggctgctgg gcctgccgcc gccgcgagac ttcatccatg gtggggggga
      181 ggtggatggc ccgctgaacc actcgcgcgg gggcttagct caagcccaag cgagatcctg
      241 caggagctgg gaaaaggctc tactcacccg caaccggggg taagcccacc tgcggcaccg
      301 gcggccccag ggccaaagga tggaccggga gaaacagacg ctttcggtaa cagtgagggc
      361 aaggagctgg tagcatcagg cgagaataag atcaagcaag gactcctgcc ttctcttgag
      421 gacctgttgt tttacacaat agccgaagga caggagaaga ttccggtaca caaatttata
      481 accgcactta agtccacagg attgagaacg tctgatccca gattgaagga gtgcatggac
      541 atgctgaggc tgacccttca aacaacttcc gacggtgtta tgctggataa ggacttgttc
      601 aagaagtgcg tacaatctaa catcgtactt ttgacgcagg cgtttagaag gaaattcgtt
      661 attcccgact ttatgtcatt caccagccat atagatgagc tctacgaaag tgctaagaaa
      721 cagtctggag ggaaagtggc agattacata ccacagctgg ccaaatttag tccagacctg
      781 tggggcgtga gtgtatgtac tgttgatggc cagcgacact ctaccggcga tacaaaagta
      841 cccttctgcc tgcagtcttg tgtcaaacct cttaagtacg ctatcgctgt caacgatctg
      901 gggacggagt acgttcatag atatgtggga aaggagccat ccggcctgcg atttaataag
      961 cttttcctta acgaggacga taaacctcat aatcccatgg taaacgccgg cgccatagtc
     1021 gtcaccagcc tcatcaagca aggtgtgaac aacgctgaaa agttcgacta cgtaatgcag
     1081 ttcctgaaca aaatggcggg taatgagtat gtaggtttct ctaacgcgac cttccagtct
     1141 gaacgggaat ccggcgaccg gaactttgcg atcggatact acctgaagga aaaaaaatgc
     1201 tttccagaag gaacagatat ggtcggaatc ctggattttt actttcaatt gtgctctatt
     1261 gaggttacat gcgaatctgc gagtgtcatg gccgcaactc ttgcgaacgg tggattttgt
     1321 ccgatcacgg gagaacgggt cttgtctcct gaagcggtga gaaataccct ctcattgatg
     1381 cattcttgtg gaatgtatga tttctctggg caattcgcct ttcatgtggg gcttcccgct
     1441 aagtccggcg tggctggcgg gattctgttg gtagtgccga acgtaatggg aatgatgtgc
     1501 tggtcccccc cgctcgacaa aatgggaaat tctgtcaagg gcattcactt ctgtcatgat
     1561 cttgtcagcc tttgtaattt ccacaattac gataacttgc gacattttgc caagaaactg
     1621 gatcctagaa gggagggagg ggatcagcgg gtgaaatcag tcattaacct cctctttgct
     1681 gcctacaccg gcgatgttag tgcactgcga aggtttgccc tttccgcaat ggatatggaa
     1741 cagcgggatt acgactccag gaccgctctt cacgtagctg ctgcggaggg ccacgttgaa
     1801 gtcgtaaaat tcctgctgga ggcgtgcaaa gtaaatccat tccccaaaga tcgctggaat
     1861 aacactccca tggacgaggc tttgcatttc ggacaccacg acgtgttcaa aattctgcag
     1921 gaataccagg ttcagtatac accccaggga gattctgaca acggaaaaga aaaccagact
     1981 gttcataaga atctcgatgg gctcctggga ggcagcgcgg ccgcaggcga ctacaaagac
     2041 catgacggtg attataaaga tcatgacatt gattacaagg atgacgatga caagtaataa
//
