LOCUS       sequence-317244-        9906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optimized enzyme for genome editing in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre
            r...
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1337..1353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1337..1353
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1373..1393
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1739..1763
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4011..4035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4040..4363
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(4367..4387)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4395..4412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4395..4411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5012..6037
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5012..6037
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     7910..7932
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7970..7988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8056..8160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8161..8229,8230..9021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8379..8398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9192..9780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9681..9700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtcggatta tgggctcttc ttttatcctc tactttcttt ctagaaaatg caaatgttga
       61 gtcagaaatt gctgaatgga gtacaactgc aatgggttta gcttatgttt ctggaacaat
      121 tactggtgga attgtgattg aaaagtaagg ttatatttca tttcaataaa tgttaccaca
      181 gtataacaaa aacaagttac attgttcaga gtatgtctga atgtattatt attattgtat
      241 ttgtgcatca gtactgtagt tgtactgaag aagggtttaa aataatctca acgtttttaa
      301 gacgagttag tgagaatacg gtcgcagtta ggatgtttgg acatagtttt attagataat
      361 cgtgaactaa ctcactataa aaacccgcta actcattgaa taagatagtt ttaaacgttc
      421 aaaatttgaa gacaaaattg tatgaataag tttacagagt tggtcgacgc aagcttctcc
      481 ttctcttcac atttctcaat aatttagctc taattgcatt cgtattcttc gcaaaaatac
      541 gtattctgat tgacccgatg aaatacggat gcctagttgc attgatcatc tacggttata
      601 cttatgggtg aggtttttca aatatatttt gatttaatcg aataatgaaa tttcagaact
      661 ggagttggtc caatttcgtg gtttatatcc tctgaactgg tacctcaaaa acatcgtagt
      721 attgctcaat ctgtggcata ttcaatgaat actgtaatgg tggttatcag tacatttaca
      781 gtattacctc tatattccgt tattggtagt tatgctttcc ttattctata ttcaattcct
      841 tcatttataa gtatgcttat attatttcgc tacttaccgg aaaccaaagg acgagagatt
      901 catgatattg tgaatgaact taaaaaaaaa tgaaaacctt tgaaaacgtt catacggccg
      961 aaattttact tgatcgagcc aattgaactt ttgttaagac ttacagaaaa gtgagagatt
     1021 ttgttacaaa aaagtgagaa aaaagcgaaa atgaacagga aaaagaagaa aaatacaccc
     1081 gaagtatcat ataaaaatta aaaagattgt ttgacctggc ggaacttttt tgcaaaaact
     1141 ctgaatatcc atggcacatg cttcgtgcaa aacaggatcc aaatgaatat caacaagaga
     1201 ttcttgaaga cgacgagcca cttgctcaga gcattgctta tctcgaatga gacccttgtt
     1261 aaaatcattt ttcaaacatt atatgacatt gtcgaaatgt cctcctgatt ccatgatggt
     1321 agcaaactca cttcgtcagg aaacagctat gaccatgatt acgccaagct atcaactttg
     1381 tatagaaaag ttggattata gtttgaagat ttctaatttc acaattagag caaatgttgt
     1441 tcggtattta ttttcaacgg tatttatact attttccacc tttttctaga acattcgagc
     1501 tgcttgttgc aaaaggaggg cgactcacat tcggtacatg gaaaagtagt gtacacaata
     1561 aagagaccca gatacatttt ccgtctgcgt ctctttgcac ccaccgggag tattttcaaa
     1621 cgaatgcatc taggaccttc tagaacattc tgtaaggctg cagaatgcgg gtatataagg
     1681 aaagcgggct cagaggaagc caacacgctt tgttctagtg catctaaaaa acttcgaaac
     1741 aagtttgtac aaaaaagcag gctaaaaatg tccaacctcc tcaccgtcca ccaaaacctc
     1801 ccagccctcc cagtcgacgc cacctccgac gaggtccgta aggtattttc ctgcattttt
     1861 caactgggaa aatgaaagaa aatcgataat ttcagaacct catggacatg ttccgtgacc
     1921 gtcaagcctt ctccgagcac acctggaaga tgctcctctc cgtctgccgt tcctgggccg
     1981 cctggtgcaa gctcaacaac cgtaagtggt tcccagccga gccaggtaag ttttcttatg
     2041 ggaaagaagg aaaaaaccga gattttactt gaaaaattga atttttcgcg ggattttcac
     2101 caaaaattgt tgaatattca ttatttcacg ctgtaaaaca aaaaaaaaaa aaatcaaaaa
     2161 ctacgttgaa atcgcgtttt taagcgaatt ttcttcagaa ttgccagatt ttaaccccaa
     2221 attttgcagt ttttaaataa aatttcacct tttcggctca aattgtagat tttcctgaaa
     2281 atttagtaca aaaaacaatt tcctcgtaaa tttttcaaat agattttcag aggacgtccg
     2341 tgactacctc ctctacctcc aagcccgtgg actcgccgtc aagaccatcc aacaacacct
     2401 cggacaactc aacatgctcc accgtcgttc cggactccca cgtccatccg actccaacgc
     2461 cgtctccctc gtcatgcgtc gtatccgtaa ggagaacgtc gacgccggag agcgtgccaa
     2521 gcaagccctc gccttcgagc gtaccgactt cgaccaagtc cgttccctca tggagaactc
     2581 cgaccgttgc caaggtaagt tatgtctacc tgcctgccta ccgcctaaat tttttgtgaa
     2641 gtttcttcaa aaaatccaga aaaaaaaaca attttcatac gattttttcc cttaaaattg
     2701 tgaattttca tgctttttag ccccaaaagt cattatttga gaaaaaattt catacaaaaa
     2761 agttttgaga aatacacaat tttttaaatg taattttcaa attttcaatt ttcaactaga
     2821 aaattcacaa aacttgtaaa ttttggacca aaacatttat acaattactt tttttgaatc
     2881 taataactac aataactaca aatttaacat tttcagacat ccgtaacctc gccttcctcg
     2941 gaatcgccta caacaccctc ctccgtatcg ccgagatcgc ccgtatccgt gtcaaggaca
     3001 tctcccgtac cgacggagga cgtatgctca tccacatcgg acgtaccaag accctcgtct
     3061 ccaccgccgg agtcgagaag gccctctccc tcggagtcac caagctcgtc gagcgttgga
     3121 tctccgtctc cggagtcgcc gacgacccaa acaactacct cttctgccgt gtccgtaagg
     3181 taagttcttt ttttgaaaag tgcagtttgt agtctaattt tcatttattt tctttttaaa
     3241 aaacgcatca atttttaaat attttgggac aaaatccgaa aactgtacta atttgtagtt
     3301 tgtaaaatta aaaaaaaaac gcaaaaaaat gttttcaaaa atgcttagaa taaaaataaa
     3361 atttctaaaa aattgaacaa aataaacatt taaaaattca aaaagtttga aaaaatgcaa
     3421 agttttttaa tgcaaaaaat atttaatgtt taaacaaatt taaaaaatgt tgtaagaata
     3481 tgtttagaga cagtttttaa atgttaaatt gcaaaaaaaa aaagcaaaaa aaaatttgaa
     3541 aaatgtaaca gaaaatttta attaaaaaag tcgtgttttt ttcaaacctg caattctttg
     3601 taattttatt tataaagaga aacaaatcaa attgtatatt acgaaaaacg atactaaaat
     3661 tcgaaaattt gcggtttttt gcgtaaaaaa tacggttccg taattttcag aacggagtcg
     3721 ccgccccatc cgccacctcc caactctcca cccgtgccct cgagggaatc ttcgaggcca
     3781 cccaccgtct catctacgga gccaaggacg actccggaca acgttacctc gcctggtccg
     3841 gacactccgc ccgtgtcgga gccgcccgtg acatggcccg tgccggagtc tccatcccag
     3901 agatcatgca agccggagga tggaccaacg tcaacatcgt catgaactac atccgtaacc
     3961 tcgactccga gacgggagcc atggtccgtc tcctcgagga cggagactag acccagcttt
     4021 cttgtacaaa gtgggataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc
     4081 ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt
     4141 ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat
     4201 gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa
     4261 cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc
     4321 caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcacaac tttattatac
     4381 atagttgata attcactggc cgtcgtttta caatttgctg atactcccgg tgaattcatg
     4441 tcttgatggt tgtgcgcatt gtggtaatat ctgaaaaatt tcgaagtcag ttttctgata
     4501 aggcaacaac taataaattg agaatcaatt tacccttaag gaacgatgtt cgcttctgaa
     4561 tttattaata aatttttccg taattcaatt caaaattgtc atatttaaga aaaaaacatc
     4621 aaaaatgaca aattcaatat tttaattaat ttttgctttc gtcgtaaatc tacacacgcg
     4681 tctcttccgt gcgagagtcc aagccagcag ccaaattcgt tgactgagta ttcaacgttt
     4741 atacgttgtc ggcaacgaga aataggaaaa tgcatcggga aatgttcttt tttcgatttt
     4801 ttccaaggtt ttgacaaatt ttaccacgaa ttttgctatg ttttcaatta aaaaatatgt
     4861 tattcaactg tttctatgag gaaaataagg ctttgcatgt aattttctta ttcagcataa
     4921 tttttaatta atttgaattt tctgtcctaa cgtttatttt gttttcttgg ttatgactga
     4981 tctgaaatta atttttgaat tttaaggtaa tatgaaaaag cctgaactca ccgcgacgtc
     5041 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5101 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5161 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5221 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5281 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5341 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5401 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5461 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5521 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5581 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5641 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5701 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5761 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5821 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5881 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     5941 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6001 ccgacgcccc agcactcgtc cgagggcaaa ggaataggtt tcccgtaatt gttacataaa
     6061 ttgttatttc aaatccttcc tttttttggt tgaaagaaaa acaatgtgat tttaaaaaaa
     6121 ttttgtgtgt tcgacttcca tttgacagac tcacttaagg ccttgactag aggtaagtgc
     6181 aagtaagatc agtgtttgtt tcgatatcag tctgtttcgt aacggtcttc tgtataacta
     6241 caaaaaagaa taaaaaaccg tatcacaact gagaaaaaag acaaaactta ttgaacagtg
     6301 ttcctttcaa aaaattattt tgtaaaaaat cgagtttttc tccaaaataa cgcacgcaat
     6361 caaaaacaag aaaactaacg tgttctcctg ggcgtggaac tccttatcaa gattaccatt
     6421 attgtctgtc agaattgata tttcaattat ttcatatcta gacaaacccg ggtgtcttgc
     6481 atttattagt actgtgagtt tacatacaaa cgaggcttac tcagataaca tagagtcacc
     6541 tctaattagc aaaaacccat ttctaattag ctatttcgac cagttctaaa gtcaccgtaa
     6601 ttagaaatgg tttttgtagc ttttctaatt agaaatgacc ccgagataag tgaaatatgg
     6661 cattttctgg gttttctagt aagaaatgat cccgcacgta gtgattccgc gttacaagcc
     6721 aaattcaaat taaaaaagta tattacacaa ttcaagttac attattttca ggaaacaatg
     6781 gcgaagagca aagcagagat tcaaaaggag gaaagagaca acagatatga acgaagactt
     6841 cttgaaaaga gaaagagaca tgaggtgagt aaccatgata ataaataatc aagtaagggc
     6901 tatagattta taaattctgg agttaacagt ttcgaaaaaa agtagttttc atatgtgcga
     6961 aggaatatca aaaatagggg aatagcctaa acgagtgaga aaattaaatg tttgttattt
     7021 tttcaaaaat gtctgcaggg ccgttatcct ttcaaatgca ctctatcaaa catagatcat
     7081 atccatcata tcttatccag aatttatatc atatccaaga ttgagagcgt ttcaaggaaa
     7141 atctcaaaca accatacaat tttgtagatc gacaacaagg cctccacttc ttcatcgatg
     7201 gcctctgcag cacctgcacc accaagtttg tatttttcat attaatataa agtgagccac
     7261 gcatagtcgt ttttaggatt tttgccagat cgatgtcgcg caaacagact gttttttttt
     7321 ctttgaaagc cgtgcgtgac atgggccacg gattgtaacg ccgcgctgga tggataaaaa
     7381 tttattcagt tttttagcct ttacaaaata tggttcgact actttcttta tttttacatt
     7441 tttataggtg ctcattgagc tgagatattt tgggtataat cggaaaatcg ctgattttgg
     7501 tcatttttct gcactaaaga ttacacaaaa agttcagtat ttagattcat tgttgaaaat
     7561 tggggatgat ctacagatac atatcgccgt gttcaaaaac accctggcgt tacccaactt
     7621 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7681 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     7741 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tgcaatctgc
     7801 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     7861 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7921 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     7981 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8161 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8221 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8281 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8341 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8401 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8461 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8521 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8581 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8641 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8701 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8761 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8821 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8881 cgcggtatca ttgcagcact ggggtcagat ggtaagccct cccgtatcgt agttatctac
     8941 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9001 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9781 aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt tgctcacatg
     9841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9901 gatacc
//
