LOCUS       sequence-317245-        3774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vitis GRF4-GIF1 in pDONR-zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik 
            JF, Dubcovsky J
  TITLE     A GRF-GIF chimeric protein improves the regeneration efficiency of 
            transgenic plants.
  JOURNAL   Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0.
  PUBMED    33046875
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0."
COMMENT     Sequence Label: JD630
FEATURES             Location/Qualifiers
     source          1..3774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     135..152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(311..338)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(430..516)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     565..587
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     579..596
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     580..596
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    612..711
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2413..2512)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2529..2548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2530..2548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2553..2569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2553..2569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(2637..3011)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3030..3077)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      3167..3755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3656..3675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
       61 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
      181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      241 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
      301 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
      361 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
      421 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
      481 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
      541 cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
      601 agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
      661 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagctgcc accatgaagc
      721 aaagctttgt gggggtttct tcacaaacag aggctgagac aagagccaga gagagtgaca
      781 cacagacaca cccacaaagg aacctcagtc actgtcccca aaaacatcct caaaacttgc
      841 aaagaaccca aaatggtggg tctgttgggg gggcatcaat ggcaacaggg atgatggtga
      901 ggtcatcacc attcacagtg tctcagtggc aggagctgga gcatcaagct ttgatcttta
      961 agtatctaat ggcaggacta cccgtcccac ctgatcttgt gcttcctatt cagaagagct
     1021 ttgaatctat gtcttctagg ttcttccacc atcctaccat gggttactgt tccttctatg
     1081 ggaagaaggt ggacccggag ccaggtagat gccggaggac tgatggcaag aaatggaggt
     1141 gctccaaaga cgcctacccg gactccaagt actgtgagcg ccacatgcac cgtggccgca
     1201 accgttcaag aaagcctgtg gaatcacaaa ctactacaca gtcttcttcg actgtgactt
     1261 cactaactgt cactggaggc agtggcagtg gagccgggac cttccagaac cttcccctgc
     1321 aaaacttcaa taacccccaa ggcggcccgg ggtccggaac caaccaatcc caattccata
     1381 tggagcccat tccctatggc attcctaata aagattacag gtatcttcat ggacttaaac
     1441 ccgaggtagg cgaacatagt ttcttctctg aagcttcagg aagcaacagg ggtcttcaga
     1501 tggactcgcc tttggacagc acatggcctc tgatgccatc acgggtctca tcattccccc
     1561 catcaaaatc aagtgaaaac tccatcttgc agagtgacta cccccagcat tcgttcttca
     1621 gcagcgaatt tgcgtcggga gaggttgtga aacaggaggg tcagtctctc cgtcctttct
     1681 ttgacgagtg gcctaaaacc agagactcct ggtccggcct tgaggaagag aggtccaacc
     1741 agacctcatt ctccacaacc cagctctcaa tttcgatccc aatggcctcc tctgacttct
     1801 caacaaccag ctctcggtct ccccacgata acgcggccgc tgccatgcag cccatgatgg
     1861 cagcctatta ccccagcaac gtcaccactg atcacattca gcagtatctt gatgaaaaca
     1921 agtcattgat tctgaagatt gttgagagcc agaattcagg aaaattgact gaatgtgcag
     1981 agaaccaggc aagactacag agaaacctca tgtacctggc tgcaatcgct gattctcaac
     2041 cccaaccacc caccatgcat gctcagttcc ctcctagtgg cattgttcag ccaggagctc
     2101 actacatgca gcaccaacaa gctcaacaaa tgacaccaca gtcgctcctg gctgcacgct
     2161 cctccatgct gtactcccaa caaccatttt cggccctgca acaacaacaa gccatccata
     2221 gccagcttgg catgggctct ggtggaagtg caggacttca catgctgcaa agcgagggga
     2281 gtaatccagg aggcaatgga acactgggga ctgatatggg aaatgcagaa gggcgaggag
     2341 ggaactcagg aggtcagggt ggggatggag gtgagactct ttacttgaaa gctgctgaag
     2401 atgggaattg acgttcagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     2461 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     2521 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     2581 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgatcag
     2641 tcctgctcct cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc
     2701 cgcccccacg gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc
     2761 gtggacacga cctccgacca ctcggcgtac agctcgtcca ggccgcgcac ccacacccag
     2821 gccagggtgt tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg
     2881 tcccggacca caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg
     2941 gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc
     3001 aacttggcca tggtttagtt cctcaccttg tcgtattata ctatgccgat atactatgcc
     3061 gatgattaat tgtcaacacg tgctgatcat gaccaaaatc ccttaacgtg agttacgcgt
     3121 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcct
//
