LOCUS       sequence-317253-       10146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optimized enzyme for genome editing in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp...
FEATURES             Location/Qualifiers
     source          1..10146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1337..1353
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1337..1353
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1373..1393
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1739..1763
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4251..4275)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4280..4603
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(4607..4627)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4635..4652)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4635..4651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5252..6277
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5252..6277
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     8150..8172
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8210..8228)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8296..8400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8401..8469,8470..9261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8619..8638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9432..10020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9921..9940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtcggatta tgggctcttc ttttatcctc tactttcttt ctagaaaatg caaatgttga
       61 gtcagaaatt gctgaatgga gtacaactgc aatgggttta gcttatgttt ctggaacaat
      121 tactggtgga attgtgattg aaaagtaagg ttatatttca tttcaataaa tgttaccaca
      181 gtataacaaa aacaagttac attgttcaga gtatgtctga atgtattatt attattgtat
      241 ttgtgcatca gtactgtagt tgtactgaag aagggtttaa aataatctca acgtttttaa
      301 gacgagttag tgagaatacg gtcgcagtta ggatgtttgg acatagtttt attagataat
      361 cgtgaactaa ctcactataa aaacccgcta actcattgaa taagatagtt ttaaacgttc
      421 aaaatttgaa gacaaaattg tatgaataag tttacagagt tggtcgacgc aagcttctcc
      481 ttctcttcac atttctcaat aatttagctc taattgcatt cgtattcttc gcaaaaatac
      541 gtattctgat tgacccgatg aaatacggat gcctagttgc attgatcatc tacggttata
      601 cttatgggtg aggtttttca aatatatttt gatttaatcg aataatgaaa tttcagaact
      661 ggagttggtc caatttcgtg gtttatatcc tctgaactgg tacctcaaaa acatcgtagt
      721 attgctcaat ctgtggcata ttcaatgaat actgtaatgg tggttatcag tacatttaca
      781 gtattacctc tatattccgt tattggtagt tatgctttcc ttattctata ttcaattcct
      841 tcatttataa gtatgcttat attatttcgc tacttaccgg aaaccaaagg acgagagatt
      901 catgatattg tgaatgaact taaaaaaaaa tgaaaacctt tgaaaacgtt catacggccg
      961 aaattttact tgatcgagcc aattgaactt ttgttaagac ttacagaaaa gtgagagatt
     1021 ttgttacaaa aaagtgagaa aaaagcgaaa atgaacagga aaaagaagaa aaatacaccc
     1081 gaagtatcat ataaaaatta aaaagattgt ttgacctggc ggaacttttt tgcaaaaact
     1141 ctgaatatcc atggcacatg cttcgtgcaa aacaggatcc aaatgaatat caacaagaga
     1201 ttcttgaaga cgacgagcca cttgctcaga gcattgctta tctcgaatga gacccttgtt
     1261 aaaatcattt ttcaaacatt atatgacatt gtcgaaatgt cctcctgatt ccatgatggt
     1321 agcaaactca cttcgtcagg aaacagctat gaccatgatt acgccaagct atcaactttg
     1381 tatagaaaag ttggattata gtttgaagat ttctaatttc acaattagag caaatgttgt
     1441 tcggtattta ttttcaacgg tatttatact attttccacc tttttctaga acattcgagc
     1501 tgcttgttgc aaaaggaggg cgactcacat tcggtacatg gaaaagtagt gtacacaata
     1561 aagagaccca gatacatttt ccgtctgcgt ctctttgcac ccaccgggag tattttcaaa
     1621 cgaatgcatc taggaccttc tagaacattc tgtaaggctg cagaatgcgg gtatataagg
     1681 aaagcgggct cagaggaagc caacacgctt tgttctagtg catctaaaaa acttcgaaac
     1741 aagtttgtac aaaaaagcag gctaaaaatg tcccaattcg acatcctctg caaggtattt
     1801 tcctgcattt ttcaactggg aaaatgaaag aaaatcgata atttcagacc ccaccaaagg
     1861 tcctcgtccg tcaattcgtc gagcgtttcg agcgtccatc cggagagaag atcgcctcct
     1921 gcgccgccga gctcacctac ctctgctgga tgatcaccca caacggaacc gccatcaagc
     1981 gtgccacctt catgtcctac aacaccatca tctccaactc cctctccttc gacatcgtca
     2041 acaagtccct ccaattcaag tacaaggtaa gttttcttat gggaaagaag gaaaaaaccg
     2101 agattttact tgaaaaattg aatttttcgc gggattttca ccaaaaattg ttgaatattc
     2161 attatttcac gctgtaaaac aaaaaaaaaa aaaatcaaaa actacgttga aatcgcgttt
     2221 ttaagcgaat tttcttcaga attgccagat tttaacccca aattttgcag tttttaaata
     2281 aaatttcacc ttttcggctc aaattgtaga ttttcctgaa aatttagtac aaaaaacaat
     2341 ttcctcgtaa atttttcaaa tagattttca gacgcaaaag gccaccatcc tcgaggcctc
     2401 cctcaagaag ctcatcccag cctgggagtt caccatcatc ccatacaacg gacaaaagca
     2461 ccaatccgac atcaccgaca tcgtctcctc cctccaactc caattcgagt cctccgagga
     2521 ggccgacaag ggaaactccc actccaagaa gatgctcaag gccctcctct ccgagggaga
     2581 gtccatctgg gagatcaccg agaagatcct caactccttc gagtacacct cccgtttcac
     2641 caagaccaag accctctacc aattcctctt cctcgccacc ttcatcaact gcggacgttt
     2701 ctccgacatc aaggtaagtt atgtctacct gcctgcctac cgcctaaatt ttttgtgaag
     2761 tttcttcaaa aaatccagaa aaaaaaacaa ttttcatacg attttttccc ttaaaattgt
     2821 gaattttcat gctttttagc cccaaaagtc attatttgag aaaaaatttc atacaaaaaa
     2881 gttttgagaa atacacaatt ttttaaatgt aattttcaaa ttttcaattt tcaactagaa
     2941 aattcacaaa acttgtaaat tttggaccaa aacatttata caattacttt ttttgaatct
     3001 aataactaca ataactacaa atttaacatt ttcagaacgt cgacccaaag tccttcaagc
     3061 tcgtccaaaa caagtacctc ggagtcatca tccaatgcct cgtcaccgag acgaagacct
     3121 ccgtctcccg tcacatctac ttcttctccg cccgtggacg tatcgaccca ctcgtctacc
     3181 tcgacgagtt cctccgtaac tccgagccag tcctcaagcg tgtcaaccgt accggaaact
     3241 cctcctccaa caagcaagag taccaactcc tcaaggacaa cctcgtccgt tcctacaaca
     3301 aggccctcaa gaagaacgcc ccatacccaa tcttcgccat caaggtaagt tctttttttg
     3361 aaaagtgcag tttgtagtct aattttcatt tattttcttt ttaaaaaacg catcaatttt
     3421 taaatatttt gggacaaaat ccgaaaactg tactaatttg tagtttgtaa aattaaaaaa
     3481 aaaacgcaaa aaaatgtttt caaaaatgct tagaataaaa ataaaatttc taaaaaattg
     3541 aacaaaataa acatttaaaa attcaaaaag tttgaaaaaa tgcaaagttt tttaatgcaa
     3601 aaaatattta atgtttaaac aaatttaaaa aatgttgtaa gaatatgttt agagacagtt
     3661 tttaaatgtt aaattgcaaa aaaaaaaagc aaaaaaaaat ttgaaaaatg taacagaaaa
     3721 ttttaattaa aaaagtcgtg tttttttcaa acctgcaatt ctttgtaatt ttatttataa
     3781 agagaaacaa atcaaattgt atattacgaa aaacgatact aaaattcgaa aatttgcggt
     3841 tttttgcgta aaaaatacgg ttccgtaatt ttcagaacgg accaaagtcc cacatcggac
     3901 gtcacctcat gacctccttc ctctccatga agggactcac cgagctcacc aacgtcgtcg
     3961 gaaactggtc cgacaagcgt gcctccgccg tcgcccgtac cacctacacc caccaaatca
     4021 ccgccatccc agaccactac ttcgccctcg tctcccgtta ctacgcctac gacccaatct
     4081 ccaaggagat gatcgccctc aaggacgaga caaacccaat cgaggagtgg caacacatcg
     4141 agcaactcaa gggatccgcc gagggatcca tccgttaccc agcctggaac ggaatcatct
     4201 cccaagaggt cctcgactac ctctcctcct acatcaaccg tcgtatctag acccagcttt
     4261 cttgtacaaa gtgggataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc
     4321 ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt
     4381 ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat
     4441 gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa
     4501 cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc
     4561 caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcacaac tttattatac
     4621 atagttgata attcactggc cgtcgtttta caatttgctg atactcccgg tgaattcatg
     4681 tcttgatggt tgtgcgcatt gtggtaatat ctgaaaaatt tcgaagtcag ttttctgata
     4741 aggcaacaac taataaattg agaatcaatt tacccttaag gaacgatgtt cgcttctgaa
     4801 tttattaata aatttttccg taattcaatt caaaattgtc atatttaaga aaaaaacatc
     4861 aaaaatgaca aattcaatat tttaattaat ttttgctttc gtcgtaaatc tacacacgcg
     4921 tctcttccgt gcgagagtcc aagccagcag ccaaattcgt tgactgagta ttcaacgttt
     4981 atacgttgtc ggcaacgaga aataggaaaa tgcatcggga aatgttcttt tttcgatttt
     5041 ttccaaggtt ttgacaaatt ttaccacgaa ttttgctatg ttttcaatta aaaaatatgt
     5101 tattcaactg tttctatgag gaaaataagg ctttgcatgt aattttctta ttcagcataa
     5161 tttttaatta atttgaattt tctgtcctaa cgtttatttt gttttcttgg ttatgactga
     5221 tctgaaatta atttttgaat tttaaggtaa tatgaaaaag cctgaactca ccgcgacgtc
     5281 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5341 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5401 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5461 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5521 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5581 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5641 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5701 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5761 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5821 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5881 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5941 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     6001 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     6061 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     6121 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6181 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6241 ccgacgcccc agcactcgtc cgagggcaaa ggaataggtt tcccgtaatt gttacataaa
     6301 ttgttatttc aaatccttcc tttttttggt tgaaagaaaa acaatgtgat tttaaaaaaa
     6361 ttttgtgtgt tcgacttcca tttgacagac tcacttaagg ccttgactag aggtaagtgc
     6421 aagtaagatc agtgtttgtt tcgatatcag tctgtttcgt aacggtcttc tgtataacta
     6481 caaaaaagaa taaaaaaccg tatcacaact gagaaaaaag acaaaactta ttgaacagtg
     6541 ttcctttcaa aaaattattt tgtaaaaaat cgagtttttc tccaaaataa cgcacgcaat
     6601 caaaaacaag aaaactaacg tgttctcctg ggcgtggaac tccttatcaa gattaccatt
     6661 attgtctgtc agaattgata tttcaattat ttcatatcta gacaaacccg ggtgtcttgc
     6721 atttattagt actgtgagtt tacatacaaa cgaggcttac tcagataaca tagagtcacc
     6781 tctaattagc aaaaacccat ttctaattag ctatttcgac cagttctaaa gtcaccgtaa
     6841 ttagaaatgg tttttgtagc ttttctaatt agaaatgacc ccgagataag tgaaatatgg
     6901 cattttctgg gttttctagt aagaaatgat cccgcacgta gtgattccgc gttacaagcc
     6961 aaattcaaat taaaaaagta tattacacaa ttcaagttac attattttca ggaaacaatg
     7021 gcgaagagca aagcagagat tcaaaaggag gaaagagaca acagatatga acgaagactt
     7081 cttgaaaaga gaaagagaca tgaggtgagt aaccatgata ataaataatc aagtaagggc
     7141 tatagattta taaattctgg agttaacagt ttcgaaaaaa agtagttttc atatgtgcga
     7201 aggaatatca aaaatagggg aatagcctaa acgagtgaga aaattaaatg tttgttattt
     7261 tttcaaaaat gtctgcaggg ccgttatcct ttcaaatgca ctctatcaaa catagatcat
     7321 atccatcata tcttatccag aatttatatc atatccaaga ttgagagcgt ttcaaggaaa
     7381 atctcaaaca accatacaat tttgtagatc gacaacaagg cctccacttc ttcatcgatg
     7441 gcctctgcag cacctgcacc accaagtttg tatttttcat attaatataa agtgagccac
     7501 gcatagtcgt ttttaggatt tttgccagat cgatgtcgcg caaacagact gttttttttt
     7561 ctttgaaagc cgtgcgtgac atgggccacg gattgtaacg ccgcgctgga tggataaaaa
     7621 tttattcagt tttttagcct ttacaaaata tggttcgact actttcttta tttttacatt
     7681 tttataggtg ctcattgagc tgagatattt tgggtataat cggaaaatcg ctgattttgg
     7741 tcatttttct gcactaaaga ttacacaaaa agttcagtat ttagattcat tgttgaaaat
     7801 tggggatgat ctacagatac atatcgccgt gttcaaaaac accctggcgt tacccaactt
     7861 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7921 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     7981 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tgcaatctgc
     8041 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     8101 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     8161 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     8221 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8281 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8341 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8401 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8461 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8521 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8581 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8641 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8701 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8761 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8821 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8881 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8941 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9001 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9061 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9121 cgcggtatca ttgcagcact ggggtcagat ggtaagccct cccgtatcgt agttatctac
     9181 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9241 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9301 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9541 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10021 aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt tgctcacatg
    10081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10141 gatacc
//
