LOCUS       sequence-317261-       13010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optimized enzyme for genome editing in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 13010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2...
FEATURES             Location/Qualifiers
     source          1..13010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    37..57
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2121..2145
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7361..7385)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10623..10643)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10651..10668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10651..10667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10660..10682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10995..11017
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11055..11073)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11141..11245
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11246..11314,11315..12106)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11464..11483)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12277..12865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12766..12785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(12992..13010,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgtgc
       61 accaatttga gtttccatgg ggaatcaagc ggataccgat tgagttgaag aaccctaaat
      121 aaaattttat atattttacc ttccaacttc gaaaaaatca ctcaccattt tagcatgttt
      181 ccctcaaaaa ccgtggcaaa ttgctcttgg aaaataatag acacatcagt tggtcgagca
      241 ttttttggag gaattttaaa actgtgatga tggttgaagg tgactatatt gtctgcgagt
      301 tgatactctg gaatattgat ttttttgaag atttcatagt cttcttggat ttcaaaaatt
      361 gaaatatttc agctgtatta aaagagaata aataaaatga tattgcctag gggtgagcgg
      421 caaacgattt tttccggcaa atcggcaaat cgacaaattg ccggaattgg aaatttccag
      481 caaatcggca tatcggcaat ttgccggaat ttaaaatttc cggcaaaccg gcaaaccggc
      541 aaattgccga tttgccgaac ttcggtaaat ttgcaaatcg gtaatttgcc gaaaattttc
      601 ggcaaattgt ggttttgcac ttattttttg gaatttcaat ttcaaatggc aaaattaaac
      661 gcatcatatg aatttttcta tatctatttt gagaagttct atgaaaatat gtagaaaaaa
      721 cgggaaaaat tcaataatag acagttttaa gtgcttccgt cttataaaaa atccctctaa
      781 acatttccgg caaatctggt attcggcaaa tcggcaagtc ggcaatttgc cgtaaattaa
      841 aatttccggc aaaacggcaa accggcaaat tgccggaatt ggaaatttcc ggcaaatcgg
      901 caaattgctt gcccattttt tgattcttag tcggcgtttt acacaaaaga taaattccaa
      961 taaaagacag aactttgctt tttgccagaa attaaaaaat tggggcaaac caatccaaat
     1021 atgaagtgta aacttgggtt tgcaccaggg ctgtgcggaa accggcaatt gccgaaattg
     1081 ccgaacccta aaaactttgg caaccggcaa cttttgctgg ctggaacttc ggcaactttt
     1141 cggcaaacgg caacttggtt gccggttgcg caaagctctg gtttgcgttt agtagcaagg
     1201 cttcctgaaa gaattccgga ggaaaactcc ccctaactca ccttttactc tatgcatctg
     1261 cttaattttg tgcaattgta gtgcaaatag taggaaatga attttttttc cgccattttt
     1321 tccttttcct atttctgctt ttcaatttcc acgtggagct cttttaaaat gaaaatttaa
     1381 actctagata tttcacgttt taaaaaaaaa aaattttaat tttctatcgg ttttactttt
     1441 gacgaaaagg gttcacagac attggaaata atcatcattt actatatata aagcgcttat
     1501 tctgtgtgtc catagtttgt agtctctgta gtctttgtag tctgtgaagt tttggcttct
     1561 ggagggatag tgagttgggg tcagtgtagg gatatagtcg gggtactgta gtggtacaat
     1621 agtggtacgg taggaatact gtagggttac ggtagtttca taaaaaatta attttcagcc
     1681 ccagaagtcg ggggccgcgc cgaaggtgcc tagacctaag cctaggcctc agcctaagcc
     1741 taattacatc aaaaaagcta tgacttttgg atcattttga cttcttttct agaacgcata
     1801 atgaaataat tcttaatttc atcggctttt tctcatcgtt gcttagaagt attgtcttga
     1861 aaattgatat ttttagaaag tacacatatt tctcaatcct tttatttaaa tttctgaaag
     1921 aaactttgcc acaacggtga gaaaagggtg cccgaaaaat gtgcatttaa atgtgaaaaa
     1981 tatccgtggc cgcggcctaa gaacccccgt cggccatgtg gtgcaagtgc gctctgttgc
     2041 ggatttttat atttaattgc attttcgcat attcgaacga ttttcctgat tttccagccg
     2101 aaattgtcca ttaaaaagtg gcaagtttgt acaaaaaagc aggctaaaaa atgtcctcct
     2161 tcgtcccaaa caaggtaagt ttatctaaaa agtttttcat tcaaaatgtg taaaaattca
     2221 tttaaaataa cccaaaaatc attaatcctc gatattttca ggagcaaacc cgtaccgtcc
     2281 tcatcttctg cttccacctc aagaagaccg ccgccgagtc ccaccgtatg ctcgtcgagg
     2341 tattttcctg catttttcaa ctgggaaaat gaaagaaaat cgataatttc aggccttcgg
     2401 agagcaagtc ccaaccgtca agacctgcga gcgttggttc caacgtttca agtccggaga
     2461 cttcgacgtc gacgacaagg agcacggaaa gccaccaaag cgttacgagg acgccgagct
     2521 ccaagccctc ctcgacgagg acgacgccca aacgcaaaag caactcgccg agcaactcga
     2581 ggtatcccaa caagccgtat ccaaccgact ccgtgagatg ggaaaggtaa gttttctgtt
     2641 ttatttactt gttttgagaa ttgggatttt tagctgaaaa attggatttt aagatgaaaa
     2701 attgaaattt tcaactaaaa atttcaattt ttagctgaaa agttaaattt ttagctaaag
     2761 agtgggattt ttagttgaaa aatcgagatt tctagctaaa aagtatgatt tttagctgaa
     2821 agattggaat tttaagcaga aaaaattgta tttttagctg ataaatcaaa ttttaagcgg
     2881 aaaaatttga atttttagct aaaaaattcc attttcaact aaaaatcgtg atttttagct
     2941 ttaaaaattg gatttttagc tgaaaaatcg gatttttagc tgaaaattga aattttttag
     3001 ttaaaaagtg agatttttta acagaaaagt gagattttta gttaaaaaat aatattttta
     3061 gctaaaaaat acaattttca actaaagatc gtgattttta gctttaaaaa ttggattttt
     3121 agctaaaaaa tcggattttt agctgaaaat tgaaattttt tagttaaaaa gtgggatttt
     3181 aagatgaaaa gtgagatttt ttagctgaaa agtgagattt tcagctaaaa aatacaattt
     3241 tcaactgaaa aattgggatt tttagcttta aaaattggat tttcagcttt aaaaattgga
     3301 tttttagctg aaaagtgaga tttttagttg aaaaattgaa atgtttagct gaaaaattgg
     3361 aatttttaag taaaaaagtg ggattttaag atgaaaagtg agatgttttt agctgaaaag
     3421 tgagattttt agctaaaaaa tacaattttc aactgaaaaa ttgggatttt tagctcaaaa
     3481 gtgggacgaa agacgaaaaa aaaaatcgat tttttaccaa aaattagatt ttcagatcca
     3541 aaaggtcgga cgttgggtcc cacacgagct caacgagcgt caaatggagc gtcgtaagaa
     3601 cacctgcgag atcctcctct cccgttacaa gcgtaagtcc ttcctccacc gtatcgtcac
     3661 cggtgacgag aagtggatct tcttcgtcaa cccaaagcgt aaggtaagtt atgtacgcga
     3721 tttctcggcg aaaaaatgga cttttttgct caaaaactga atttttcagc tttttttgca
     3781 ctgaaaatgc tgaaaattgg tttttttgct gaaaattcac cgaaaaaagc tgaaaatatt
     3841 gcagaaatcg acgaaaaatg ccttttttgc tgaaatttca gccagaaaat tgttcagaat
     3901 cgcaatttcc tgccaaaatc gacgtttttc ggcgaaaatt ggtagatttt gaaccgaaaa
     3961 ttgtgaaaaa tggacttttt tgctcaaaaa cagacttttt gagctttttt cacactgaaa
     4021 atggtggaaa attggatttt ccagcagaaa attcggaaat tttcaatttt tggcggaaat
     4081 ttgacgaaaa atatatgaaa aacaccgatt tttgtctgaa aaagttcaaa agccgaaaaa
     4141 ttggccaaaa atctccaaaa tcggactttt ctggcgtttt tcgtggaaaa attgaaattt
     4201 ttttgaaaaa ttgagcaaaa atcgccgaaa aaccgctaaa aaaactgccg aaattggcaa
     4261 ttttgagctt aaaaaaactt gaaaaatgct caaaattacc caattttcac cggaaattga
     4321 gctaaaatcg tcgaaaaaga ctgaattttc aatttttagc tgaaaatctg cctattttcg
     4381 gctaaaattg ctttaaaaac attaaaattc aaattttttg gctaaaaata ctcagattgc
     4441 cctttttttt ctccaaaaat gacaaaaaaa aaatcaatca gaaaaatgcg atttttttta
     4501 caaaaattgg cttcaaaacg tctaaattct ccctaatttt tgctgctgaa aattgtgcaa
     4561 aaatcaccga aaaatgacga ttttaggctg aaaatcttca aaaaaagctc aaaattcccc
     4621 acttttcacc ggaaaaagac gaaaaaagct ccaaatttat cgattaacat tttcagaagt
     4681 cctacgtcga cccaggacaa ccagccacct ccaccgcccg tccaaaccgt ttcggaaaga
     4741 agaccatgct ctgcgtctgg tgggaccaat ccggagtcat ctactacgag ctcctcaagc
     4801 caggtaagtt cttttttttg ctcgtttttg agtgaaaaat tgatggaatt cacaaaaaat
     4861 ggccaaagaa tcctcaaaat tggccgaaaa ttctgaaaaa tgacctaaaa atcaccgaaa
     4921 aacggccaaa aattatctga aaatcgccga aaatctcaaa ttttgaggaa attcccgcta
     4981 aaaattaata aaaatcaccg aaaaaaggcc aaaaattacc taataaaggc cgaaaatcgc
     5041 ctaaaaaggc caaaaattct gaaaaatggc caaatttcaa tttttcacac acaaaaaatc
     5101 cctgaatttt ctatttttac tcgaaaaact gtggatttac gggggtaaaa attcagtttt
     5161 tagtgctatt tcacaagaaa aacacagcat tttcgacaaa aactccgatt tttaagccaa
     5221 aaatttgagg tttttagttt tttacgtgaa aacctgaact ttttgccaaa aaaacacttt
     5281 tttttagact aaaaatcaat tttcgatcaa aaatcactaa ttttgcctga aaaaatatag
     5341 atttttccac tttttcactg aaaaaaaact ggattttcac tagaaaatca agttgttttt
     5401 agaccaaaaa ttttattttt ccgctgaaaa ttatcgtttt tcagtcaatt ttcagctaaa
     5461 aatccctgaa attctggatt ttcagccaaa aaactcgaaa ttttccaaaa aaaccccgtt
     5521 taaaaatgag tttagactga aaattccgat ttttgagcca aaaatttgat ttttaatcga
     5581 aaaaaattgg attttcaacc aaaaaatcag tttttcagtg atttttagag taaaaatcac
     5641 tgattttaac acaaaattcc agtttttcgt gatttttcac tagaaaatcc cgatttttcc
     5701 ccaaaaaatc catttttccg actaaaaatc cccaaagtaa ttttcaggag aaaccgtcaa
     5761 cactgcccgt taccagcaac agctcatcaa tctcaaccgt gccctccaac gtaagcgtcc
     5821 agagtaccaa aagcgtcaac atcgtgtcat cttcctccac gacaacgccc catcccacac
     5881 ggcccgtgcc gtccgtgaca ccctcgagac actcaactgg gaggtcctcc cacacgccgc
     5941 ctactcccca ggtaagttca tacaattttc tggagaaaaa tattttaaaa aataacgaaa
     6001 atggaccgaa tttttggaga aaattttaaa aaaaacgaca tttttcccaa aaaattgaaa
     6061 aatttccgaa aaaacgttca aaattttcaa aaaaggagca tttttcgaaa aaaaaaacga
     6121 taatacggaa attttttgat tttccagttt ttttctcaaa gaaaatcgga aaaaggtttt
     6181 ttttctttaa aaacgtccaa aatttttcga aaaaagagaa aattttcgat tttccgattt
     6241 ttttcagaaa aaaattgaaa aaaaaagaaa ttttccgatt ttctcagaga aagacgaaaa
     6301 gacgatattt ttcggaaaaa agttgaaaaa tcaaaaaaaa aaccgaaaaa cggccgaaat
     6361 tttctaaaaa ttggaaatat tgtcgatttt tcgaaaaaaa agagtcaaaa gttttcaaaa
     6421 aaatatattt tttcgatttt ccgttttttt ttttcagtaa acattgaaac aaacgaaatt
     6481 tttccaaaaa aatccaatta ttttcccaaa tatcgaaata tttttgattt tccgtttttt
     6541 tcagaaaaaa gttaaaaaaa aaagggaaaa ttaagatttt cctgattttt tttgagaaaa
     6601 aatcgataaa aattgaatat tctggaaaaa acctttaaaa aattcgaatt aaatcgaaaa
     6661 acgtccaaaa ttttctaaaa aattgaaatt ttttttgaaa aaaaacggaa aaatttagat
     6721 ttcccgactt ttttcaatga aaaatcgaat tttcgttgac ttttcattaa aaaatccaaa
     6781 cgcatttttt tccactaaaa tctcgaaaaa gtgtcatatt gcaccatatt gtttccacat
     6841 tcatattatt ttgagctaaa tcaggtgatt ttctgtattt tgatactaat ttctattttt
     6901 agaaaaaatg tgggaacaat atggtgcaat atgacatttt ttcgagattt tagtggagaa
     6961 aaaatgcgtt tggaaacgaa ttttataata aaaagtcaac gaaaattcga tttttcatgg
     7021 aaaaataggg aaatcgaaaa caaatttgct tttttttcaa aaaaaaaaac ggaaattcga
     7081 aatttttccg ttctttcaaa aaaaaatcga tttttttgga aatttttttt ttcaaatttt
     7141 taaaaagaat ttttcagacc tcgccccatc cgactaccac ctcttcgcct ccatgggaca
     7201 cgccctcgcc gagcaacgtt tcgactccta cgagtccgtc aagaagtggc tcgacgagtg
     7261 gttcgccgcc aaggacgacg agttctactg gcgtggaatc cacaagctcc cagagcgttg
     7321 ggagaagtgc gtcgcctccg acggaaagta cttcgagtaa acccagcttt cttgtacaaa
     7381 gtggcctaag ctgtctcatc ctactttcac ctagttaact gcttgtctta aaatctatgc
     7441 ttctctttag tatctaaaat tttcctagaa gcttacaagt atataaatgg tctcttctca
     7501 ataaaggttg tatatttatt catcttattg aatctgccat ttcctcgttt ttgcgagttt
     7561 atataccttc caattttctt tctattgtat tttcaacttc taattttaat tcagggaaac
     7621 tgcttcaacg catcatgggt tgtaccatgt cacaggaaga gcgtgccgct cttgaaagat
     7681 cacgaatgtc ctcagtctcc aaggtaacac ttagtttttg ttgctctgtt taaaaattaa
     7741 ttttaaggga gaggagctca tcaaggagaa catgcacatg aagctctaca tggagggaac
     7801 cgtcaacaac caccacttca agtgcacctc cgagggagag ggaaagccat acgagggaac
     7861 ccaaaccatg cgtatcaagg tcgtcgaggg aggaccactc ccattcgcct tcgacatcct
     7921 cgccacctcc ttcatgtacg gatcgcgtac cttcatcaac cacacccaag gtaagttttc
     7981 ttatgggaaa gaaggaaaaa accgagattt tacttgaaaa attgaatttt tcgcgggatt
     8041 ttcaccaaaa attgttgaat attcattatt tcacgctgta aaacaaaaaa aaaaaaaatc
     8101 aaaaactacg ttgaaatcgc gtttttaagc gaattttctt cagaattgcc agattttaac
     8161 cccaaatttt gcagttttta aataaaattt caccttttcg gctcaaattg tagattttcc
     8221 tgaaaattta gtacaaaaaa caatttcctc gtaaattttt caaatagatt ttcaggaatc
     8281 ccagacttct tcaagcaatc cttcccagag ggattcacct gggagcgtgt caccacctac
     8341 gaggacggag gagtcctcac cgccacccaa gacacctccc tccaagacgg atgcctcatc
     8401 tacaacgtca aggtaagtta tgtctacctg cctgcctacc gcctaaattt tttgtgaagt
     8461 ttcttcaaaa aatccagaaa aaaaaacaat tttcatacga ttttttccct taaaattgtg
     8521 aattttcatg ctttttagcc ccaaaagtca ttatttgaga aaaaatttca tacaaaaaag
     8581 ttttgagaaa tacacaattt tttaaatgta attttcaaat tttcaatttt caactagaaa
     8641 attcacaaaa cttgtaaatt ttggaccaaa acatttatac aattactttt tttgaatcta
     8701 ataactacaa taactacaaa tttaacattt tcagatccgt ggagtcaact tcccatccaa
     8761 cggaccagtc atgcaaaaga agaccctcgg atgggaggcc aacaccgaga tgctctaccc
     8821 agccgacgga ggactggagg gacgtaccga catggccctc aagctcgtcg gaggaggaca
     8881 cctcatctgc aacttcaaga ccacctaccg ttccaagaag ccagccaagg taagttcttt
     8941 ttttgaaaag tgcagtttgt agtctaattt tcatttattt tctttttaaa aaacgcatca
     9001 atttttaaat attttgggac aaaatccgaa aactgtacta atttgtagtt tgtaaaatta
     9061 aaaaaaaaac gcaaaaaaat gttttcaaaa atgcttagaa taaaaataaa atttctaaaa
     9121 aattgaacaa aataaacatt taaaaattca aaaagtttga aaaaatgcaa agttttttaa
     9181 tgcaaaaaat atttaatgtt taaacaaatt taaaaaatgt tgtaagaata tgtttagaga
     9241 cagtttttaa atgttaaatt gcaaaaaaaa aaagcaaaaa aaaatttgaa aaatgtaaca
     9301 gaaaatttta attaaaaaag tcgtgttttt ttcaaacctg caattctttg taattttatt
     9361 tataaagaga aacaaatcaa attgtatatt acgaaaaacg atactaaaat tcgaaaattt
     9421 gcggtttttt gcgtaaaaaa tacggttccg taattttcag aacctcaaga tgccaggagt
     9481 ctactacgtc gaccaccgtc tggagcgtat caaggaggcc gacaaggaga cgtacgtcga
     9541 gcaacacgag gtcgccgtcg cccgttactg cgacctccca tccaagctcg gacacaagct
     9601 caacggaatg gacgagctct acaagtcggg ataattttcc ccagatttcc ccacgatttt
     9661 ttttgacatt tttatgaaaa ttttctctaa aagttacccc tattttgttt agactgatct
     9721 cttattttat ccaatgtttc aaatttttga aattgctcga aaattccaat tttcacatat
     9781 ttttgttgat tttttcgcca ttttcccctg ataaattaac ttatttttgc aattttttct
     9841 ttcgaaaaaa tattggtttc tcatcaccat ttatcctttt ttttatggag ttaaaaattg
     9901 ttaaaaaaat ttaatttttt ttctaatttt agagtcaaaa cgggttcgaa tctagagcgt
     9961 agctgtacga ttttttggcg atttttgcgg ttttctggtg agttttttta aagttatttt
    10021 tttgaaaaaa attcttgaaa ataaaaggaa attgttttaa aaaaaaattt caaagaaaaa
    10081 attttgaaaa aattgaaaca ttttttttat tttgaccaaa aatattgaaa attaattaac
    10141 attttttttt ggaaagttaa tttttaatac aaatttttta aatttatttt acaaaaaaaa
    10201 ttgaatttct gtgaaaaatc agttttccag tacataatga tttgaccaaa aaaaattgaa
    10261 aaaaatgatt ttttaaaatt aaaaatacca taaaatgctg aaaattcaga aaaataagta
    10321 aattttaaaa aattctggaa aaacatctaa aaacccccca aatataatat tttggaccct
    10381 tttattttct gctcgacatc tcaaatatcc tccaattttt cgcttttttg attaattgaa
    10441 agcatagtgg gggcaggtgt tataggacac ccttccctcc acttttttgg agcatttttc
    10501 aaagctcttc cgagagcaga agcttctcaa tttttccacc ccaaaagttt gaaattctgc
    10561 caaaaaatct cgcattttaa caatttttcg agatgtttca cgtccttttc attgcgattt
    10621 cccaacttta ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg
    10681 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    10741 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10801 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    10861 atggtgcact ctcagtgcaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
    10921 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10981 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    11041 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    11101 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    11161 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    11221 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    11281 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    11341 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    11401 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    11461 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    11521 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    11581 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    11641 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    11701 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    11761 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    11821 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    11881 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    11941 atctggagcc ggtgagcgtg gctctcgcgg tatcattgca gcactggggt cagatggtaa
    12001 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    12061 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    12121 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    12181 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    12241 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    12301 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    12361 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    12421 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    12481 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    12541 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    12601 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    12661 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    12721 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    12781 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    12841 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt
    12901 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    12961 ccgtattacc gcctttgagt gagctgatac cagcggataa caatttcaca
//
