LOCUS       sequence-317274-       10442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mTurquoise2-tagged iLID anchored to the 
            plasma membrane via N-terminal fusion to lipidated peptide from LYN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Natwick DE, Collins SR
  TITLE     Optimized iLID Membrane Anchors for Local Optogenetic Protein 
            Recruitment.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1009-1023. doi: 
            10.1021/acssynbio.0c00511. Epub 2021 Apr 12.
  PUBMED    33843200
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00511"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1009-1023"
COMMENT     Sequence Label: pLV_Lyn11-mTurquoise2-iLID
FEATURES             Location/Qualifiers
     source          1..10442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2899..3110
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3111..4049
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4006..4026
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(4144..4146,4147..4857)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4186..4207)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4447..4466)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4794..4815
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4879..5316
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     misc_feature    5407..5956
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5551..5568)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5778..5797
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6411..6999
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6464..6484)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6882..6893)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7206..7839
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7967..7983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7967..7983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7980..8002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7991..8007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8015..8021,8022..8039,8040..8045))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8060..8081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8198..8215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8369..8957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8449..8468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9128..9919,9920..9988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9751..9770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9989..10093)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10141..10275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10178..10197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10232..10251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cacatatgcg ggaggtggtc
     2221 cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt
     2281 tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt
     2341 ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga
     2401 cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa
     2461 gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg
     2521 aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca
     2581 ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga
     2641 ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc
     2701 ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg ggcgcgcgcc
     2761 cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc cccggcggcc
     2821 ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta ggcgcggaga
     2881 ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2941 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3001 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3061 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3121 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3181 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3241 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaagcctggc
     3301 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3361 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3421 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3481 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3541 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3601 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3661 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3721 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3781 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3841 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3901 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3961 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4021 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg gatccgcgca aatgggatgt
     4081 ataaaatcaa aagggaaaga cagcgcggga gcagatagtg ctggtagtgc tggtagtgct
     4141 ggtgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4201 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4261 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4321 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     4381 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4441 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4501 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4561 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
     4621 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     4681 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4741 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4801 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
     4861 agtggtagtg gggagtttct ggcaaccaca ctggaacgga tcgagaaaaa tttcgtgatt
     4921 actgatccga gactgcctga caacccaatc atttttgcga gcgattcctt cctgcagctg
     4981 acagaatatt ctcgggaaga gatcctgggg cgcaattgcc gttttctgca gggacccgag
     5041 acagaccgtg ccactgttcg gaaaatcaga gatgctattg acaaccagac tgaagtgacc
     5101 gttcagctga tcaattatac caagagcggc aagaagttct ggaacgtgtt ccacctgcag
     5161 ccgatgcgcg attataaggg cgacgtccag tacttcattg gcgtgcagct ggatggcacc
     5221 gaacgtcttc atggcgccgc tgagcgtgag gcggtctgcc tgatcaaaaa gacagccttt
     5281 cagattgctg aggcagcgaa cgacgaaaat tacttttaga cgcgtcgggc cgcggtaaca
     5341 attgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc
     5401 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5461 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5521 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5581 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5641 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5701 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5761 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5821 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5881 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     5941 gaaaaacacg ataataccat ggtcatgaaa acatttaaca tttctcaaca agatctagaa
     6001 ttagtagaag tagcgacaga gaagattaca atgctttatg aggataataa acatcatgtg
     6061 ggagcggcaa ttcgtacgaa aacaggagaa atcatttcgg cagtacatat tgaagcgtat
     6121 ataggacgag taactgtttg tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga
     6181 caaaaggatt ttgacacgat tgtagctgtt agacaccctt attctgacga agtagataga
     6241 agtattcgag tggtaagtcc ttgtggtatg tgtagggagt tgatttcaga ctatgcacca
     6301 gattgttttg tgttaataga aatgaatggc aagttagtca aaactacgat tgaagaactc
     6361 attccactca aatatacccg aaattaaccc gggcggggcg cgtctggaac aatcaacctc
     6421 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6481 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6541 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6601 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6661 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6721 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6781 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6841 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6901 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6961 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     7021 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7081 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     7141 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     7201 gggactggaa gggctaattc gctcccaacg aagacaagat atccttgatc tgtggatcta
     7261 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7321 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7381 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     7441 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7501 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     7561 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     7621 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     7681 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7741 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7801 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7861 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     7921 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8041 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8161 ggagaggcgg tttgtgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10141 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10201 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10261 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10321 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10381 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10441 gt
//
