LOCUS       sequence-317277-       11683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mTurquoise2-tagged iLID anchored to the 
            plasma membrane via N-terminal fusion to ADRB2 for slow diffusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Natwick DE, Collins SR
  TITLE     Optimized iLID Membrane Anchors for Local Optogenetic Protein 
            Recruitment.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1009-1023. doi: 
            10.1021/acssynbio.0c00511. Epub 2021 Apr 12.
  PUBMED    33843200
REFERENCE   2  (bases 1 to 11683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00511"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1009-1023"
COMMENT     Sequence Label: pLV_ADRB2-mTurquoise2-iLID
FEATURES             Location/Qualifiers
     source          1..11683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2899..3110
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3111..4049
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4006..4026
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4120..4143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(5386..5388,5389..6099)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5428..5449)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5689..5708)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6036..6057
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6121..6558
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     misc_feature    6648..7197
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6792..6809)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7019..7038
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    7652..8240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7705..7725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8123..8134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8447..9080
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9208..9224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9208..9224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9221..9243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9232..9248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9256..9262,9263..9280,9281..9286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9301..9322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9439..9456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9610..10198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9690..9709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10369..11160,11161..11229))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10992..11011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11230..11334)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11382..11516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11419..11438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11473..11492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cacatatgcg ggaggtggtc
     2221 cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt
     2281 tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt
     2341 ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga
     2401 cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa
     2461 gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg
     2521 aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca
     2581 ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga
     2641 ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc
     2701 ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg ggcgcgcgcc
     2761 cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc cccggcggcc
     2821 ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta ggcgcggaga
     2881 ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2941 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3001 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3061 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3121 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3181 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3241 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaagcctggc
     3301 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3361 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3421 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3481 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3541 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3601 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3661 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3721 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3781 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3841 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3901 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3961 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4021 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg gatccgcgca aatgaagacg
     4081 atcatcgccc tgagctacat cttctgcctg gtattcgccg actacaagga cgacgatgac
     4141 aaggggcaac ccgggaacgg cagcgccttc ttgctggcac ccaatagaag ccatgcgccg
     4201 gaccacgacg tcacgcagca aagggacgag gtgtgggtgg tgggcatggg catcgtcatg
     4261 tctctcatcg tcctggccat cgtgtttggc aatgtgctgg tcatcacagc cattgccaag
     4321 ttcgagcgtc tgcagacggt caccaactac ttcatcactt cactggcctg tgctgatctg
     4381 gtcatgggcc tggcagtggt gccctttggg gccgcccata ttcttatgaa aatgtggact
     4441 tttggcaact tctggtgcga gttttggact tccattgatg tgctgtgcgt cacggccagc
     4501 attgagaccc tgtgcgtgat cgcagtggat cgctactttg ccattacttc acctttcaag
     4561 taccagagcc tgctgaccaa gaataaggcc cgggtgatca ttctgatggt gtggattgtg
     4621 tcaggcctta cctccttctt gcccattcag atgcactggt accgggccac ccaccaggaa
     4681 gccatcaact gctatgccaa tgagacctgc tgtgacttct tcacgaacca agcctatgcc
     4741 attgcctctt ccatcgtgtc cttctacgtt cccctggtga tcatggtctt cgtctactcc
     4801 agggtctttc aggaggccaa aaggcagctc cagaagattg acaaatctga gggccgcttc
     4861 catgtccaga accttagcca ggtggagcag gatgggcgga cggggcatgg actccgcaga
     4921 tcttccaagt tctgcttgaa ggagcacaaa gccctcaaga cgttaggcat catcatgggc
     4981 actttcaccc tctgctggct gcccttcttc atcgttaaca ttgtgcatgt gatccaggat
     5041 aacctcatcc gtaaggaagt ttacatcctc ctaaattgga taggctatgt caattctggt
     5101 ttcaatcccc ttatctactg ccggagccca gatttcagga ttgccttcca ggagcttctg
     5161 tgcctgcgca ggtcttcttt gaaggcctat gggaatggct actccagcaa cggcaacaca
     5221 ggggagcaga gtggatatca cgtggaacag gagaaagaaa ataaactgct gtgtgaagac
     5281 ctcccaggca cggaagactt tgtgggccat caaggtactg tgcctagcga taacattgat
     5341 tcacaaggga ggaattgtag tacaaatgac tcactgctgg gatccgtgag caagggcgag
     5401 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5461 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5521 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc
     5581 tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     5641 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5701 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5761 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5821 tttagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     5881 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     5941 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6001 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6061 gccgccggga tcactctcgg catggacgag ctgtacaagg gtagtggtag tggggagttt
     6121 ctggcaacca cactggaacg gatcgagaaa aatttcgtga ttactgatcc gagactgcct
     6181 gacaacccaa tcatttttgc gagcgattcc ttcctgcagc tgacagaata ttctcgggaa
     6241 gagatcctgg ggcgcaattg ccgttttctg cagggacccg agacagaccg tgccactgtt
     6301 cggaaaatca gagatgctat tgacaaccag actgaagtga ccgttcagct gatcaattat
     6361 accaagagcg gcaagaagtt ctggaacgtg ttccacctgc agccgatgcg cgattataag
     6421 ggcgacgtcc agtacttcat tggcgtgcag ctggatggca ccgaacgtct tcatggcgcc
     6481 gctgagcgtg aggcggtctg cctgatcaaa aagacagcct ttcagattgc tgaggcagcg
     6541 aacgacgaaa attactttta gacgcgtcgg gccgcggtaa caattgttaa ctaacttaag
     6601 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc ccccctaacg ttactggccg
     6661 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     6721 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     6781 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     6841 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     6901 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     6961 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     7021 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     7081 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     7141 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca
     7201 tggtcatgaa aacatttaac atttctcaac aagatctaga attagtagaa gtagcgacag
     7261 agaagattac aatgctttat gaggataata aacatcatgt gggagcggca attcgtacga
     7321 aaacaggaga aatcatttcg gcagtacata ttgaagcgta tataggacga gtaactgttt
     7381 gtgcagaagc cattgcgatt ggtagtgcag tttcgaatgg acaaaaggat tttgacacga
     7441 ttgtagctgt tagacaccct tattctgacg aagtagatag aagtattcga gtggtaagtc
     7501 cttgtggtat gtgtagggag ttgatttcag actatgcacc agattgtttt gtgttaatag
     7561 aaatgaatgg caagttagtc aaaactacga ttgaagaact cattccactc aaatataccc
     7621 gaaattaacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     7681 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7741 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7801 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7861 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7921 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7981 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     8041 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     8101 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     8161 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     8221 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     8281 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     8341 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     8401 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     8461 cgctcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8521 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     8581 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     8641 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     8701 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     8761 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     8821 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     8881 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8941 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9001 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9061 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9121 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     9181 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgtgtat
     9421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11341 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    11401 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11461 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    11521 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11581 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11641 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
