LOCUS       sequence-317280-       11387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mTurquoise2-tagged iLID anchored to the 
            plasma membrane via N-terminal fusion to Stargazin for slow 
            diffusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Natwick DE, Collins SR
  TITLE     Optimized iLID Membrane Anchors for Local Optogenetic Protein 
            Recruitment.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1009-1023. doi: 
            10.1021/acssynbio.0c00511. Epub 2021 Apr 12.
  PUBMED    33843200
REFERENCE   2  (bases 1 to 11387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00511"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1009-1023"
COMMENT     Sequence Label: pLV_Stargazin-mTurquoise2-iLID
FEATURES             Location/Qualifiers
     source          1..11387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2899..3110
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3111..4049
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4006..4026
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(5089..5091,5092..5802)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5131..5152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5392..5411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5739..5760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5824..6261
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     misc_feature    6352..6901
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6496..6513)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6723..6742
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    7356..7944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7409..7429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7827..7838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8151..8784
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8912..8928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8912..8928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8925..8947)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8936..8952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8960..8966,8967..8984,8985..8990))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9005..9026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9143..9160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9314..9902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9394..9413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10073..10864,10865..10933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10696..10715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10934..11038)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11086..11220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11123..11142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11177..11196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cacatatgcg ggaggtggtc
     2221 cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt
     2281 tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt
     2341 ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga
     2401 cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa
     2461 gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg
     2521 aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca
     2581 ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga
     2641 ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc
     2701 ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg ggcgcgcgcc
     2761 cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc cccggcggcc
     2821 ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta ggcgcggaga
     2881 ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2941 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3001 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3061 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3121 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3181 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3241 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaagcctggc
     3301 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3361 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3421 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3481 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3541 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3601 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3661 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3721 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3781 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3841 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3901 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3961 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4021 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg gatccgcgca aatggggctg
     4081 tttgatcgag gtgttcaaat gcttttaacc accgttggtg ctttcgctgc cttcagcttg
     4141 atgaccatcg ctgtgggaac cgactattgg ctgtactcca gaggggtttg caagaccaaa
     4201 agtgtcagtg agaatgaaac cagcaagaag aacgaggaag ttatgaccca ttccggatta
     4261 tggagaacct gctgcctcga agggaacttc aaaggtctgt gcaagcaaat cgaccacttt
     4321 ccggaagacg cggactacga agctgacacc gcagagtatt tcctccgggc cgtgagggcc
     4381 tcgagtatct tcccgatcct gagtgtgatc ctgcttttca tgggtggcct ctgcatcgca
     4441 gccagcgagt tctacaaacc ggtgaactgc aggaagagct cgaagaattc gaaaagcttg
     4501 acacgccaca acatcatcct gagtgctggc atcttcttcg tgtctgcagg tcttagtaat
     4561 atcatcggga tcatcgtgta tatatcagcc aatgctggag acccctccaa gagtgactcc
     4621 aaaaagaaca gctactccta cggctggtcc ttctacttcg gggccctgtc cttcatcatc
     4681 gccgagatgg tcggggtgct ggccgtgcac atgtttatcg accgccacaa acagctgcgg
     4741 gccacggccc gcgccaccga ctacctccag gcctccgcca tcacccgcat ccccagctac
     4801 cgctaccgct accagcgccg cagccgctcc agctcgcgct ccaccgagcc ctctcactcc
     4861 agagacgcct cgcccgtggg cgtgaagggc ttcaacaccc tgccgtccac ggagatctcc
     4921 atgtacaccc tcagtaggga ccccctgaag gctgccacca cgcccaccgc cacctacaac
     4981 tcggacaggg ataacagctt tctccaggtc cacaactgta tccagaagga cagcaaggac
     5041 tctttccacg ccaacacagc caaccgccgg accacgcccg taggatccgt gagcaagggc
     5101 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5161 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5221 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5281 tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     5341 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5401 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5461 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5521 tactttagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     5581 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     5641 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5701 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5761 accgccgccg ggatcactct cggcatggac gagctgtaca agggtagtgg tagtggggag
     5821 tttctggcaa ccacactgga acggatcgag aaaaatttcg tgattactga tccgagactg
     5881 cctgacaacc caatcatttt tgcgagcgat tccttcctgc agctgacaga atattctcgg
     5941 gaagagatcc tggggcgcaa ttgccgtttt ctgcagggac ccgagacaga ccgtgccact
     6001 gttcggaaaa tcagagatgc tattgacaac cagactgaag tgaccgttca gctgatcaat
     6061 tataccaaga gcggcaagaa gttctggaac gtgttccacc tgcagccgat gcgcgattat
     6121 aagggcgacg tccagtactt cattggcgtg cagctggatg gcaccgaacg tcttcatggc
     6181 gccgctgagc gtgaggcggt ctgcctgatc aaaaagacag cctttcagat tgctgaggca
     6241 gcgaacgacg aaaattactt ttagacgcgt cgggccgcgg taacaattgt taactaactt
     6301 aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg
     6361 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     6421 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     6481 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     6541 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     6601 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     6661 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     6721 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     6781 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     6841 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat
     6901 accatggtca tgaaaacatt taacatttct caacaagatc tagaattagt agaagtagcg
     6961 acagagaaga ttacaatgct ttatgaggat aataaacatc atgtgggagc ggcaattcgt
     7021 acgaaaacag gagaaatcat ttcggcagta catattgaag cgtatatagg acgagtaact
     7081 gtttgtgcag aagccattgc gattggtagt gcagtttcga atggacaaaa ggattttgac
     7141 acgattgtag ctgttagaca cccttattct gacgaagtag atagaagtat tcgagtggta
     7201 agtccttgtg gtatgtgtag ggagttgatt tcagactatg caccagattg ttttgtgtta
     7261 atagaaatga atggcaagtt agtcaaaact acgattgaag aactcattcc actcaaatat
     7321 acccgaaatt aacccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt
     7381 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7441 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7501 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7561 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7621 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7681 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7741 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     7801 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7861 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7921 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     7981 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     8041 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     8101 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     8161 aattcgctcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     8221 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     8281 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     8341 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     8401 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     8461 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     8521 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     8581 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8761 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8821 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt
     8881 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8941 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9001 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9061 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgt
     9121 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9181 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9241 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9721 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10381 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10561 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10681 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10981 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11041 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
    11101 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11161 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11221 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11281 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    11341 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
