LOCUS       sequence-317290-        8312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA1-boxB-MS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pLH-sgRNA1-boxB-MS2
FEATURES             Location/Qualifiers
     source          1..8312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             590..895
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     774..793
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    928..946
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     misc_RNA        988..1006
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_feature    1085..1202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1251..1761
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1680..1698
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1851..2876
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1851..2876
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(2900..2916)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2901..2917)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2999..3232
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3304..3425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3341..3360)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3395..3414
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3465..3600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3620..3639)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3621..3639)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3649..3666)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3649..3665)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3658..3680)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3807..4262
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3894..3913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4104..4125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4288..4392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4393..4461,4462..5253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4611..4630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5424..6012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5913..5932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6166..6183
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6300..6321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6336..6341,6342..6359,6360..6366)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6374..6390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6379..6401
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6398..6414
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6398..6414
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6433..6453
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6435..6453
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6481..6707
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6708..6888
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6935..7060
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7553..7786
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7971..8015
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gtgtcttcga ggcttactaa aagccagata acagtatgcg tatttgcgcg
      301 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
      361 gtgtgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
      421 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
      481 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
      541 tttattgaaa tgaacggctc ttttgctgac gagaacaggg actggtgaaa tgcagtttaa
      601 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
      661 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
      721 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
      781 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
      841 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaaccct
      901 gcaggacgaa gacccgtttg agagctaggg ccctgaagaa gggccctagc aagttcaaat
      961 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
     1021 ggcaccgagt cggtgctttt tttgaattct cgacctcgag acaaatggca gtattcatcc
     1081 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     1141 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     1201 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     1261 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     1321 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     1381 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     1441 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     1501 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     1561 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     1621 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     1681 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     1741 atcaccgacc tctctcccca gggggatcca ccggatcaag cttgccagct ggggcgccct
     1801 ctggtaaggt tgggaagccc tgcagatctg atcccggggg gcaatgagat atgaaaaagc
     1861 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     1921 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     1981 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     2041 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta
     2101 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagatctgc
     2161 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     2221 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     2281 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     2341 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     2401 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     2461 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     2521 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     2581 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     2641 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     2701 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     2761 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     2821 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta
     2881 gatgccgacc gggatctatc gataccgtcg acctcgaggg ggggcccggt acctttaaga
     2941 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     3001 gaagggctaa ttcactccca acgaagaaaa gatctgcttt ttgcttgtac tgggtctctc
     3061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     3241 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     3301 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3361 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3421 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     3481 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3541 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3601 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     3661 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3721 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3781 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4021 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4141 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4201 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4261 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4381 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4441 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4621 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5221 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6181 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6241 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6301 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6361 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6421 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     6481 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     6541 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     6601 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     6661 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     6721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     6901 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     6961 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     7021 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     7081 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     7141 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     7201 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7261 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7321 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7381 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7441 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7501 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7561 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7621 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7681 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7741 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7801 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7861 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7921 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7981 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     8041 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     8101 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     8161 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     8221 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     8281 gagactagcc tcgagcggcc gcccccttca cc
//
