LOCUS       sequence-317291-        9036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector under the CMV promoter that expresses 
            smURFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 9036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pLenti-smURFP
FEATURES             Location/Qualifiers
     source          1..9036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..23)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      209..344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     271..290
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        406..453
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             472..846
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    976..1097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1013..1032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1067..1086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1146..1162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1146..1162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1159..1181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1170..1186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1194..1200,1201..1218,1219..1224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1239..1260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1377..1394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1548..2136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1628..1647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2307..3098,3099..3167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2930..2949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3168..3272)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3347..3366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3538..3917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3919..4117
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4135..4315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4362..4487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4980..5213
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5398..5442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5740..5857
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        5909..6288
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6289..6492
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6537..6556
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6537..6555
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6575..6584
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6581..6982
                     /codon_start=1
                     /product="dimeric small ultra-red fluorescent protein 
                     derived from a cyanobacterial phycobiliprotein (Rodriguez 
                     et al., 2016)"
                     /label=smURFP
                     /translation="MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAF
                     SQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRC
                     LKEASLSLLDEEDANETAPYFDYIIKAMS"
     misc_feature    7007..7595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7060..7080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7478..7489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7598..7614)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7599..7615)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8120..8300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8326..8343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8332..8556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8602..9030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8689..8708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8899..8920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
       61 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      121 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      181 tccgcccatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
      301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      361 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
      421 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
      481 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
      541 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
      601 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
      661 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
      721 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
      781 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
      841 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
      901 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
      961 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1021 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1081 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     1141 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1201 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1261 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1321 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1981 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3301 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     3361 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     3421 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     3481 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     3541 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3601 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3781 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3841 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3901 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4021 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4081 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     4141 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4201 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4261 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     4321 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     4381 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     4441 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     4501 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     4561 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     4621 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     4681 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     4741 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     4801 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     4861 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     4921 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     4981 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     5041 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     5101 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     5161 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     5221 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     5281 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     5341 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     5401 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     5461 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     5521 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     5581 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     5641 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     5701 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     5761 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     5821 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     5881 agatccagtt tggttaatta aacgcgttga cattgattat tgactagtta ttaatagtaa
     5941 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     6001 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     6061 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     6121 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     6181 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     6241 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     6301 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     6361 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     6421 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     6481 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     6541 acgactcact atagggagac ccaagctggg atccgccacc atggctaaga cttccgaaca
     6601 gagggtgaac attgctacac tgctgacaga aaataagaag aaaatcgtgg ataaggcttc
     6661 ccaggatctg tggcggagac acccagacct gatcgcacca ggaggaattg ctttctctca
     6721 gagggaccgc gctctgtgcc tgcgagatta cggctggttc ctgcatctga tcaccttttg
     6781 tctgctggcc ggagataagg gccccatcga gtctattggg ctgatcagta ttcgagaaat
     6841 gtataactca ctgggagtgc ccgtccctgc aatgatggag agcattagat gcctgaaaga
     6901 agccagcctg tccctgctgg acgaagagga cgccaacgag accgcaccct actttgatta
     6961 cattattaag gctatgagct aagaattcga tatcaagctt atcgataatc aacctctgga
     7021 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7081 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7141 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7201 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7261 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7321 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7381 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7441 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7501 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7561 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     7621 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     7681 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     7741 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7801 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     7861 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     7921 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     7981 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     8041 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     8101 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8161 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8221 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8281 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     8341 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8401 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8461 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8521 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     8581 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8641 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8701 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8761 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8821 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8881 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     8941 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9001 agctgattta acaaaaattt aacgcgaatt aattct
//
