LOCUS       sequence-317292-        9435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMma PylT(UUA)_FLAG-AcKRS
FEATURES             Location/Qualifiers
     source          1..9435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2727..2938
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2951..3219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3269..3278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3278..3301
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4685..5259,5260..5262,5263..5271)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4852..4869)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5079..5098
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5284..5883
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5284..5303)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5780..5800
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5943..5963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6005..6126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6042..6061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6096..6115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6889..6923
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7414..7430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7414..7430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7427..7449)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7438..7454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7462..7468,7469..7486,7487..7492))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7507..7528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7645..7662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7816..8404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7896..7915)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8575..9366,9367..9435))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9198..9217
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc wytyctckgg gmnctgtggg cgatgtgcgc tctgcccact
     1321 gacgggcacc ggagcgatcg cagatccttt ctagagcact agttctgcag tatttagcat
     1381 gccccaccca tctgcaaggc attctggata gtgtcaaaac agccggaaat caagtccgtt
     1441 tatctcaaac tttagcattt tgggaataaa tgatatttgc tatgctggtt aaattagatt
     1501 ttagttaaat ttcctgctga agctctagta cgataagtaa cttgacctaa gtgtaaagtt
     1561 gagatttcct tcaggtttat atagcttgtg cgccgcctgg gtacctcgga aacctgatca
     1621 tgtagatcga acggacttta aatccgttca gccgggttag attcccgggg tttccggaca
     1681 agtgcggttt ttcctgcagt atttagcatg ccccacccat ctgcaaggca ttctggatag
     1741 tgtcaaaaca gccggaaatc aagtccgttt atctcaaact ttagcatttt gggaataaat
     1801 gatatttgct atgctggtta aattagattt tagttaaatt tcctgctgaa gctctagtac
     1861 gataagtaac ttgacctaag tgtaaagttg agatttcctt caggtttata tagcttgtgc
     1921 gccgcctggg tacctcggaa acctgatcat gtagatcgaa cggactttaa atccgttcag
     1981 ccgggttaga ttcccggggt ttccggacaa gtgcggtttt tcctgcagta tttagcatgc
     2041 cccacccatc tgcaaggcat tctggatagt gtcaaaacag ccggaaatca agtccgttta
     2101 tctcaaactt tagcattttg ggaataaatg atatttgcta tgctggttaa attagatttt
     2161 agttaaattt cctgctgaag ctctagtacg ataagtaact tgacctaagt gtaaagttga
     2221 gatttccttc aggtttatat agcttgtgcg ccgcctgggt acctcggaaa cctgatcatg
     2281 tagatcgaac ggactttaaa tccgttcagc cgggttagat tcccggggtt tccggacaag
     2341 tgcggttttt cctgcagtat ttagcatgcc ccacccatct gcaaggcatt ctggatagtg
     2401 tcaaaacagc cggaaatcaa gtccgtttat ctcaaacttt agcattttgg gaataaatga
     2461 tatttgctat gctggttaaa ttagatttta gttaaatttc ctgctgaagc tctagtacga
     2521 taagtaactt gacctaagtg taaagttgar wttwmcywgc atagcaaata tcatttattc
     2581 ccaaaatgyw aarkttkrrr wwaamsgrmn ttgatttccg gctgttttga cactatccag
     2641 aatgccttgc agatgggtgg ggcatgctaa atactgcaga actagtgctc tagaaaggat
     2701 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2761 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     2821 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2881 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     2941 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     3001 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     3061 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     3121 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     3181 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     3241 tctagagcta gcgtttaaac ttaagcttgc caccatggac tacaaggacg acgacgacaa
     3301 gatggacaag aagcccctga acaccctgat cagcgccaca ggactgtgga tgtccagaac
     3361 cggcaccatc cacaagatca agcaccacga ggtgtcccgg tccaaaatct acatcgagat
     3421 ggcctgcggc gatcacctgg tcgtcaacaa cagcagaagc agccggacag ccagagccct
     3481 gcggcaccac aagtacagaa agacctgcaa gcggtgcaga gtgtccgacg aggacctgaa
     3541 caagttcctg accaaggcca acgaggacca gaccagcgtg aaagtgaagg tggtgtccgc
     3601 ccccacccgg accaagaaag ccatgcccaa gagcgtggcc agagccccca agcccctgga
     3661 aaacaccgaa gccgctcagg cccagcccag cggcagcaag ttcagccccg ccatccccgt
     3721 gtctacccag gaaagcgtca gcgtccccgc cagcgtgtcc accagcatct ctagcatctc
     3781 aaccggcgcc acagcttctg ccctggtcaa gggcaacacc aaccccatca ccagcatgtc
     3841 tgcccctgtg caggcctctg ccccagccct gaccaagtcc cagaccgacc ggctggaagt
     3901 gctcctgaac cccaaggacg agatcagcct gaacagcggc aagcccttcc gggagctgga
     3961 aagcgagctg ctgagccggc ggaagaagga cctccagcaa atctacgccg aggaacggga
     4021 gaactacctg ggcaagctgg aaagagagat cacccggttc ttcgtggacc ggggcttcct
     4081 ggaaatcaag agccccatcc tgatccccct ggagtacatc gagcggatgg gcatcgacaa
     4141 cgacaccgag ctgagcaagc agattttccg ggtggacaag aacttctgcc tgcggcccat
     4201 gatggccccc aacatcttca actacgcccg gaaactggat cgcgctctgc ccgaccccat
     4261 caagattttc gagatcggcc cctgctaccg gaaagagagc gacggcaaag agcacctgga
     4321 agagtttaca atgctgaact ttttccagat gggcagcggc tgcaccagag agaacctgga
     4381 atccatcatc accgactttc tgaaccacct ggggatcgac ttcaagatcg tgggcgacag
     4441 ctgcatggtg tacggcgaca ccctggacgt gatgcacggc gacctggaac tgtctagcgc
     4501 cgtcgtggga cccatccctc tggaccggga gtggggcatc gataagccct ggatcggagc
     4561 cggcttcggc ctggaacggc tgctgaaagt caagcacgac tttaagaaca tcaagcgggc
     4621 tgccagaagc gagagctact acaacggcat cagcaccaac ctgtgataag gatccgcggc
     4681 cgcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4741 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4801 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4861 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4921 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4981 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5041 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5101 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5161 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5221 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggcgtcc
     5281 ggaatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5341 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5401 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5461 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5521 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5581 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5641 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     5701 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     5761 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     5821 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     5881 tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5941 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgcgtta
     6001 actaaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6121 atcttatcat gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg
     6181 gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac
     6241 tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga
     6301 acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt
     6361 ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa
     6421 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     6481 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     6541 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     6601 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     6661 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     6721 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     6781 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     6841 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     6901 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
     6961 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
     7021 gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
     7081 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
     7141 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
     7201 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
     7261 acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt
     7321 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
     7381 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7441 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7501 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7561 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7621 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7681 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7741 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7801 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7861 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7921 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7981 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8041 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8101 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8161 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8221 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8281 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8341 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8401 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8461 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8521 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8581 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8641 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8701 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8761 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8821 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8881 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8941 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9001 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9061 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9121 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9181 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9241 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9301 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9361 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9421 atgttgaata ctcat
//
