LOCUS       sequence-317331-        8250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon optimized 
            AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and 
            P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Walton RT, Hsu JY, Joung JK, Kleinstiver BP
  TITLE     Scalable characterization of the PAM requirements of CRISPR-Cas 
            enzymes using HT-PAMDA.
  JOURNAL   Nat Protoc. 2021 Feb 5. pii: 10.1038/s41596-020-00465-2. doi: 
            10.1038/s41596-020-00465-2.
  PUBMED    33547443
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2021 Feb 5. pii: 10.1038/s41596-020-00465-2. doi: 
            10.1038/s41596-020-00465-2."
COMMENT     Sequence Label: pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)
FEATURES             Location/Qualifiers
     source          1..8250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             658..4578
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4298..4307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4579..4626
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4633..4713
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4732..4788
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4789..4791,4792..4794,4795..5508)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4789..4791,4792..4794,4795..5505)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4834..4855)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5095..5114)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5442..5463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5514..5531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5554..5571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5560..5784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5855..5871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5855..5871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5868..5890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5879..5895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5903..5909,5910..5927,5928..5933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5948..5969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6086..6103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6257..6845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6337..6356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7016..7807,7808..7876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7639..7658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7877..7981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8060..8079)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 acacagttcg agggctttac caacctgtat caggtgagca agacactgcg gtttgagctg
      721 atcccacagg gcaagaccct gaagcacatc caggagcagg gcttcatcga ggaggacaag
      781 gcccgcaatg atcactacaa ggagctgaag cccatcatcg atcggatcta caagacctat
      841 gccgaccagt gcctgcagct ggtgcagctg gattgggaga acctgagcgc cgccatcgac
      901 tcctatagaa aggagaaaac cgaggagaca aggaacgccc tgatcgagga gcaggccaca
      961 tatcgcaatg ccatccacga ctacttcatc ggccggacag acaacctgac cgatgccatc
     1021 aataagagac acgccgagat ctacaagggc ctgttcaagg ccgagctgtt taatggcaag
     1081 gtgctgaagc agctgggcac cgtgaccaca accgagcacg agaacgccct gctgcggagc
     1141 ttcgacaagt ttacaaccta cttctccggc ttttatgaga acaggaagaa cgtgttcagc
     1201 gccgaggata tcagcacagc catcccacac cgcatcgtgc aggacaactt ccccaagttt
     1261 aaggagaatt gtcacatctt cacacgcctg atcaccgccg tgcccagcct gcgggagcac
     1321 tttgagaacg tgaagaaggc catcggcatc ttcgtgagca cctccatcga ggaggtgttt
     1381 tccttccctt tttataacca gctgctgaca cagacccaga tcgacctgta taaccagctg
     1441 ctgggaggaa tctctcggga ggcaggcacc gagaagatca agggcctgaa cgaggtgctg
     1501 aatctggcca tccagaagaa tgatgagaca gcccacatca tcgcctccct gccacacaga
     1561 ttcatccccc tgtttaagca gatcctgtcc gataggaaca ccctgtcttt catcctggag
     1621 gagtttaaga gcgacgagga agtgatccag tccttctgca agtacaagac actgctgaga
     1681 aacgagaacg tgctggagac agccgaggcc ctgtttaacg agctgaacag catcgacctg
     1741 acacacatct tcatcagcca caagaagctg gagacaatca gcagcgccct gtgcgaccac
     1801 tgggatacac tgaggaatgc cctgtatgag cggagaatct ccgagctgac aggcaagatc
     1861 accaagtctg ccaaggagaa ggtgcagcgc agcctgaagc acgaggatat caacctgcag
     1921 gagatcatct ctgccgcagg caaggagctg agcgaggcct tcaagcagaa aaccagcgag
     1981 atcctgtccc acgcacacgc cgccctggat cagccactgc ctacaaccct gaagaagcag
     2041 gaggagaagg agatcctgaa gtctcagctg gacagcctgc tgggcctgta ccacctgctg
     2101 gactggtttg ccgtggatga gtccaacgag gtggaccccg agttctctgc ccggctgacc
     2161 ggcatcaagc tggagatgga gccttctctg agcttctaca acaaggccag aaattatgcc
     2221 accaagaagc cctactccgt ggagaagttc aagctgaact ttcagatgcc tacactggcc
     2281 tctggctggg acgtgaataa ggagaagaac aatggcgcca tcctgtttgt gaagaacggc
     2341 ctgtactatc tgggcatcat gccaaagcag aagggcaggt ataaggccct gagcttcgag
     2401 cccacagaga aaaccagcga gggctttgat aagatgtact atgactactt ccctgatgcc
     2461 gccaagatga tcccaaagtg cagcacccag ctgaaggccg tgacagccca ctttcagacc
     2521 cacacaaccc ccatcctgct gtccaacaat ttcatcgagc ctctggagat cacaaaggag
     2581 atctacgacc tgaacaatcc tgagaaggag ccaaagaagt ttcagacagc ctacgccaag
     2641 aaaaccggcg accagaaggg ctacagagag gccctgtgca agtggatcga cttcacaagg
     2701 gattttctgt ccaagtatac caagacaacc tctatcgatc tgtctagcct gcggccatcc
     2761 tctcagtata aggacctggg cgagtactat gccgagctga atcccctgct gtaccacatc
     2821 agcttccaga gaatcgccga gaaggagatc atggatgccg tggagacagg caagctgtac
     2881 ctgttccaga tctataacaa ggactttgcc aagggccacc acggcaagcc taatctgcac
     2941 acactgtatt ggaccggcct gttttctcca gagaacctgg ccaagacaag catcaagctg
     3001 aatggccagg ccgagctgtt ctaccgccct aagtccagga tgaagaggat ggcacaccgg
     3061 ctgggagaga agatgctgaa caagaagctg aaggatcaga aaaccccaat ccccgacacc
     3121 ctgtaccagg agctgtacga ctatgtgaat cacagactgt cccacgacct gtctgatgag
     3181 gccagggccc tgctgcccaa cgtgatcacc aaggaggtgt ctcacgagat catcaaggat
     3241 aggcgcttta ccagcgacaa gttctttttc cacgtgccta tcacactgaa ctatcaggcc
     3301 gccaattccc catctaagtt caaccagagg gtgaatgcct acctgaagga gcaccccgag
     3361 acacctatca tcggcatcga tcggggcgag agaaacctga tctatatcac agtgatcgac
     3421 tccaccggca agatcctgga gcagcggagc ctgaacacca tccagcagtt tgattaccag
     3481 aagaagctgg acaacaggga gaaggagagg gtggcagcaa ggcaggcctg gtctgtggtg
     3541 ggcacaatca aggatctgaa gcagggctat ctgagccagg tcatccacga gatcgtggac
     3601 ctgatgatcc actaccaggc cgtggtggtg ctggagaacc tgaatttcgg ctttaagagc
     3661 aagaggaccg gcatcgccga gaaggccgtg taccagcagt tcgagaagat gctgatcgat
     3721 aagctgaatt gcctggtgct gaaggactat ccagcagaga aagtgggagg cgtgctgaac
     3781 ccataccagc tgacagacca gttcacctcc tttgccaaga tgggcaccca gtctggcttc
     3841 ctgttttacg tgcctgcccc atatacatct aagatcgatc ccctgaccgg cttcgtggac
     3901 cccttcgtgt ggaaaaccat caagaatcac gagagccgca agcacttcct ggagggcttc
     3961 gactttctgc actacgacgt gaaaaccggc gacttcatcc tgcactttaa gatgaacaga
     4021 aatctgtcct tccagagggg cctgcccggc tttatgcctg catgggatat cgtgttcgag
     4081 aagaacgaga cacagtttga cgccaagggc acccctttca tcgccggcaa gagaatcgtg
     4141 ccagtgatcg agaatcacag attcaccggc agataccggg acctgtatcc tgccaacgag
     4201 ctgatcgccc tgctggagga gaagggcatc gtgttcaggg atggctccaa catcctgcca
     4261 aagctgctgg agaatgacga ttctcacgcc atcgacacca tggtggccct gatccgcagc
     4321 gtgctgcaga tgcggaactc caatgccgcc acaggcgagg actatatcaa cagccccgtg
     4381 cgcgatctga atggcgtgtg cttcgactcc cggtttcaga acccagagtg gcccatggac
     4441 gccgatgcca atggcgccta ccacatcgcc ctgaagggcc agctgctgct gaatcacctg
     4501 aaggagagca aggatctgaa gctgcagaac ggcatctcca atcaggactg gctggcctac
     4561 atccaggagc tgcgcaacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
     4621 aaaaagggat cctacccata cgatgttcca gattacgctt atccctacga cgtgcctgat
     4681 tatgcatacc catatgatgt ccccgactat gccggaggcg gtggaagcgg cgcaacaaac
     4741 ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat ggtgagcaag
     4801 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4861 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4921 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4981 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5041 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5101 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5161 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5221 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5281 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5341 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     5401 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5461 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgacc ggtcatcatc
     5521 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5581 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5641 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5701 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5761 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     5821 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5881 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     5941 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6001 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6061 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6181 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6241 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6301 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6361 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6421 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6481 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6541 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6601 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6661 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6721 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6781 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6841 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6901 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6961 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7021 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7081 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7141 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7201 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7261 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7321 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7381 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7441 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7501 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7561 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7621 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7681 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7741 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7801 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7861 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7921 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7981 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     8041 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8101 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     8161 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     8221 ttcgcgatgt acgggccaga tatacgcgtt
//
