LOCUS       sequence-31739-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for nuclear expression of small RNAs in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Good PD, Krikos AJ, Li SX, Bertrand E, Lee NS, Giver L, Ellington A,
            Zaia JA, Rossi JJ, Engelke DR
  TITLE     Expression of small, therapeutic RNAs in human cell nuclei.
  JOURNAL   Gene Ther. 1997 Jan . 4(1):45-54.
  PUBMED    9068795
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Ther. 
            1997 Jan . 4(1):45-54."
COMMENT     Sequence Label: pAV U6+27
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..258
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10..30
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     181..200
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     polyA_signal    405..526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(442..461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     496..515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgggcaggag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
       61 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
      121 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      181 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      241 tggaaaggac gaaacaccgt gctcgcttcg gcagcacata tactagtcga cgggtctaga
      301 gcggacttcg gtccgctttt tactaggacc tgcaggcatg caagcttgac gtcggttacc
      361 gatatccata tggcggccgc atcgatctcg agccgcggac tagtaacttg tttattgcag
      421 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      481 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtcttacgta
      541 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
      601 actcctctct gcgcgctcg
//
