LOCUS       sequence-317394-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV plasmid to induce expression of micro-RNA targeted against 
            mouse Mecp2 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ito-Ishida A, Baker SA, Sillitoe RV, Sun Y, Zhou J, Ono Y, Iwakiri 
            J, Yuzaki M, Zoghbi HY
  TITLE     MeCP2 Levels Regulate the 3D Structure of Heterochromatic Foci in 
            Mouse Neurons.
  JOURNAL   J Neurosci. 2020 Nov 4;40(45):8746-8766. doi: 
            10.1523/JNEUROSCI.1281-19.2020. Epub 2020 Oct 12.
  PUBMED    33046553
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.1281-19.2020"; journalName: "J Neurosci"; date: 
            "2020-11-4- 4"; volume: "40"; issue: "45"; pages: "8746-8766"
COMMENT     Sequence Label: pAAV-CAG-GFP-mirMecp2
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     242..263
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           368..394
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           458..499
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(587..607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cnggggnnna gctggagtac anctacaaca gccacaacgt ctatatcatg gccgacaagc
       61 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      121 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      181 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      241 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      301 acaagtaagc taagcacttc gtggccgtcg atcgtttaaa gggaggtagt gagtcgacca
      361 gtggatcctg gaggcttgct gaaggctgta tgctgttcac ctgaacacct tctgatgttt
      421 tggccactga ctgacatcag aaggttcagg tgaacaggac acaaggcctg ttactagcac
      481 tcacatggaa caaatggccc agatctggcc gcactcgaat caagcttatc gataatcaac
      541 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
      601 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
      661 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
      721 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
      781 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
      841 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
      901 ctganaattc cgtggtnttg tcggggaaat catcgtcctt tcctggctgc tcgctgtgtt
      961 gccnctggat ctnnngggac gtcttctgct ngtccttcgg ccnaatcagn nnacttcttc
     1021 cgcggctggt gcggnntgng gcnttccggt tncgctnnnn tnaannnnng atnccttggg
     1081 cgnnnccnan nnnnnncncc gggnc
//
