LOCUS       sequence-317397-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV plasmid to induce expression of universal negative control 
            micro-RNA and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ito-Ishida A, Baker SA, Sillitoe RV, Sun Y, Zhou J, Ono Y, Iwakiri 
            J, Yuzaki M, Zoghbi HY
  TITLE     MeCP2 Levels Regulate the 3D Structure of Heterochromatic Foci in 
            Mouse Neurons.
  JOURNAL   J Neurosci. 2020 Nov 4;40(45):8746-8766. doi: 
            10.1523/JNEUROSCI.1281-19.2020. Epub 2020 Oct 12.
  PUBMED    33046553
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.1281-19.2020"; journalName: "J Neurosci"; date: 
            "2020-11-4- 4"; volume: "40"; issue: "45"; pages: "8746-8766"
COMMENT     Sequence Label: pAAV-CAG-GFP-mirNega
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     238..259
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           364..390
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           454..495
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(583..603)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggnnnagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
       61 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      121 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      181 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      241 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      301 gtaagctaag cacttcgtgg ccgtcgatcg tttaaaggga ggtagtgagt cgaccagtgg
      361 atcctggagg cttgctgaag gctgtatgct gaaatgtact gcgcgtggag acgttttggc
      421 cactgactga cgtctccacg cagtacattt caggacacaa ggcctgttac tagcactcac
      481 atggaacaaa tggcccagat ctggccgcac tcgaatcaag cttatcgata atcaacctct
      541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
      601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
      661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
      721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
      781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
      841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
      901 caattccgng gngttgtcgg ggaaatcatc gtcctttcnt ggctgcnnnc tgtgtgcncc
      961 tggatntnnn nggacnnnct ctgcnngtcc ttcggccnaa tcagcggact tcttccnngg
     1021 ctgnngcggt nngggncntt cggnttnnnc tnnccnnann nnnnnnannn cttgggccnt
     1081 nccgnnnann nncnccgggg nnntcganaa annaaaaa
//
