LOCUS       sequence-317402-        3747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  citrus GRF4-GIF1 in pDONR-zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik 
            JF, Dubcovsky J
  TITLE     A GRF-GIF chimeric protein improves the regeneration efficiency of 
            transgenic plants.
  JOURNAL   Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0.
  PUBMED    33046875
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0."
COMMENT     Sequence Label: JD689
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     97..114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(273..300)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(392..478)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     527..549
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     541..558
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     542..558
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    574..673
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2348..2447)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2464..2483)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2465..2483)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2488..2504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2488..2504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(2572..2946)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2965..3012)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      3102..3690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3591..3610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      121 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      181 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
      241 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
      301 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
      361 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
      421 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
      481 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
      541 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
      601 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
      661 aaaaaagctg ccaccatgaa tagcggcaac ggcggtggcg gtgctggtgg ggctggtggg
      721 ttggggatga tgggaatgag ggcatcacct tttacggtat cacaatggca agaactggaa
      781 catcaagctt tgatctttaa gtacatggtg gcaggtttgc ctgtgccacc tgatcttgta
      841 ctccctatac aaaagagctt tgagtctatt tctcataggt tctttcatca ccccaccatg
      901 ggttattgtt ccttctatgg gaaaaaagtt gatcccgaac caggacggtg caggaggact
      961 gacggcaaaa aatggcggtg ttccaaagat gcatacccag actcaaagta ctgtgagcgc
     1021 cacatgcacc gcggccgcaa ccgttcaaga aagcctgtgg aatcacaaac cataacacag
     1081 tcatcatcca ctgtgacatc actgactgcc actggaagca gtggtgggac tggaagctac
     1141 cagaaccttc cattacatgc ctttgctaat ccccaaggca ctacttctgg aaccaaccaa
     1201 tcccattatc atgtggactc catttcctgt ggaattccca acaaagaata caggtacctt
     1261 caagttcaag gacttaaacc cgaggttgga gagcatagtt tcttcactga agcttcagga
     1321 agcaataggg gtctccagat ggactcacca attgacagtg catggcctct gatgcaatct
     1381 agagtctcct cgcatcccca gtcgaaatcc agcaacacct ccattttgca gaatgattat
     1441 cctcagcatt cacttttcag tagtgaattc acctcgggtg agcctgtaaa acaggaaggt
     1501 cagtctctcc gacctttctt tgatgagtgg cctaagacaa gagactcatg gtctggtctt
     1561 gaagacaaga gatcccacca gacctcattc tcaacaacca agctctctat atccatccct
     1621 atggcctcat ccgacttctc ggcaacgagt tctcgatcgc ctcatgataa cgcggccgct
     1681 gccatgcaac agcacctgat gcagatgcag cccatgatgg cagcttatta tcccaacaac
     1741 gtcactactg accacattca acagtatcta gatgagaaca aatcattgat tttgaagatt
     1801 gttgagagcc agaattcagg gaaactgagc gagtgtgcag agaaccaggc aagattgcag
     1861 cggaatctca tgtacctggc tgctattgct gatgctcaac cccaaccacc tagcgttcat
     1921 gcccagttct cttctggtgg cattatgcag ccaggagctc actatatgca acaccagcaa
     1981 tctcagccaa tgacaccaca gtcacttatg gctgcacgct catccatggt gtactctcaa
     2041 cagcaatttt cagtgcttca gcaacagcaa gccttgcatg gtcagcttgg catgagctct
     2101 ggtggtagct caggacttca catgctgcaa agtgagggta gtactgcagg aggtagtggt
     2161 tcacttgggg gtgggggatt ccctgatttt ggccgtggct catctggtga aggcttgcac
     2221 tcaaggggaa tggggagcaa gcatgatata ggcagttctg gatctgctga aggacgagga
     2281 gggagctcag gaagccaaga tggaggcgaa actctctact tgaaaggggc tgatgatgga
     2341 aattaacgtt cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2401 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2461 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2521 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg atcagtcctg
     2581 ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga actcccgccc
     2641 ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga agttcgtgga
     2701 cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca cccaggccag
     2761 ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg
     2821 gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc ggtcggtcca
     2881 gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt
     2941 ggccatggtt tagttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga
     3001 ttaattgtca acacgtgctg atcatgacca aaatccctta acgtgagtta cgcgtcgttc
     3061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3241 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3481 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3721 ctggcctttt gctggccttt tgctcac
//
