LOCUS       sequence-317405-         174 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast low copy plasmid with dCas9, sgRNA expression cassette and 
            VP64 activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 174)
  AUTHORS   Camara E, Lenitz I, Nygard Y
  TITLE     A CRISPR activation and interference toolkit for industrial 
            Saccharomyces cerevisiae strain KE6-12.
  JOURNAL   Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.
  PUBMED    32884066
REFERENCE   2  (bases 1 to 174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-3"; volume: "10(1):14605. doi"; pages: " 
            10.1038/s41598-020-71648-w"
COMMENT     Sequence Label: EC2_2_dCas9_VP64_sgRNA
FEATURES             Location/Qualifiers
     source          1..174
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..150
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             154..174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
       61 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
      121 gcccttgatg atttcgacct ggacatgctg attccgaaaa agaaacgcaa agtt
//
