LOCUS       sequence-317420-        9516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018363
FEATURES             Location/Qualifiers
     source          1..9516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2220..2240
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2241..2264
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2290..2346
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2347..2363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2356..2378)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2572..2591)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2691..2713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2751..2769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3555..4754
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4985..6133)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6405..6776)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6809..6918
                     /note="incP origin of transfer"
     rep_origin      7071..7702
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8165..8753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8654..8673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8907..8924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9041..9062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9077..9082,9083..9100,9101..9107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9115..9131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9120..9142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9139..9155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9139..9155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcccc
      361 gccgtgagcg cacgcgctgc gtcagcgacc gccgccgctt gttcgtggtc gcagtgggcg
      421 gatcgctctt cctggccggc tgcaagggcg acaaggagaa ggacgacccg cgcgtggccc
      481 tggccgcgcg ggtcggtccc gccacgacca gcggcgacac caccacgaag ttcgcggatg
      541 acggccagtg ggtgcggccg gcgaaggact tccagggtac ccgcttcagc gggctcaccg
      601 agatcaacaa cacgaacgtt cagaagcttc acgtcttcgg caccttcagc ttggggacga
      661 cccgcggcgt ggaggcggcg cccatcgtgg ccggcacgac gatgtatatc gtcacgccgt
      721 tccccaactt cgtctacgcc ctggacctca cgaaggaggg tatgccgacc aagtggagct
      781 acaagccgcg cccggtcaac gccgcccagg gcgtggcgtg ctgcgacgtg gtcaaccgcg
      841 gtctcgtagt cgatgccgga aaggtgatct tcaataccct ggacggccgc accattgccc
      901 tggacgcagc cacgggcaag gagctctggg agacccagct ggcgaacatc aacctgggcg
      961 agtccgtgac gatggccccc ctcgtggtga agggcaaggt gttcgtgggg gtgtccggcg
     1021 gcgagttcgg cgtgcgcggg tggctgaaag cggtggacga gaactccggc aagctcgcgt
     1081 ggacggccta ccacaccggc cccgacaaag acgtcctcat cgggcccaac ttcaagccct
     1141 tctataagat ggaccagggc aaggatctcg gcgtgtccag ctggccggcc gaccagtgga
     1201 agatcggcgg cgccggcgtt tggggctgga tcacctacga tcccgagctg gacctcatct
     1261 actacgggac ggccaacccc ggaccctgga accccgagat gcgtcccggc gataataagt
     1321 ggagctccac ggtgttcgcg cgccatccgg ataacgggga agccgtctgg gcgtaccagc
     1381 tgtcccctca cgacgagcac gactatgatg gcgtcaacga gaacatcctc gtggacatgg
     1441 actggaaggg tacgcgccgg cagctcatcc ttcgcccgga acgcaacggc tacttctacg
     1501 tgttcgaccg gaaaaccggg gaagtcctct ccgcggaacc cttcggcttc gtcaacacga
     1561 ccacgggcgt ggacctgaag acgggccgcc tgcaatatgc gcccgagaag gcgacgcgca
     1621 tcggccaggt ggtgcgctac tcctgcccgg cagccccggg catgaaggat tggcagccga
     1681 gcgcgtactc cccgcgcacg ggcctggtgt acatccccca tcagaacctt tgcatggacg
     1741 aagaggggct cgaggcgaac tatatcgccg gcacgcccta tgtggggacc aacgtcaaga
     1801 tgtacgcggg ccccggcggg aaccgtgggt tcttcgatgc ctgggacccg gtcggccagc
     1861 acaaggtctg gagcatcaag gaagatctgc cggtgtggag tggggcgctc gtgacggcag
     1921 gcgatgtggc cttttacggc acgatggatg gctggttcaa ggcggtcgac gcgcgcaccg
     1981 gcaacccgct ctggcagttc aagacggggt ccggcatcat ctcgcagccg atgacctact
     2041 cgtatggggg taagcagtac atcgccgtcc tcagcggcgt cggcggctgg tcgggcgcca
     2101 tcgtcgccgg cggcctggac gcccgggaca gctcggccgc cctgggtttc gtgaacgcca
     2161 tgaaggacct gccgcagaag tcgaccaagg gtggcatgct gtacgtgttc gccctggccg
     2221 aaaacctcta ttttcaggga catcatcacc atcaccatca ccactgacag ctatgaccat
     2281 gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
     2341 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2401 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2461 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     2521 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     2581 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     2641 acgggcttgt atgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     2701 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     2761 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagcacc ctttctcggt
     2821 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
     2881 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
     2941 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
     3001 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     3061 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     3121 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
     3181 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     3241 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     3301 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     3361 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     3421 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     3481 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     3541 tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc actgtcgcgc
     3601 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     3661 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttggtgc
     3721 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct
     3781 tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca gcgcctttcc
     3841 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     3901 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     3961 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     4021 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     4081 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     4141 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     4201 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     4261 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     4321 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     4381 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     4441 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     4501 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     4561 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     4621 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     4681 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     4741 gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag gcgacttccg
     4801 gcaagctata cgcgccctag aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
     4861 agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt
     4921 gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa
     4981 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
     5041 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
     5101 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
     5161 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
     5221 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
     5281 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
     5341 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
     5401 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
     5461 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
     5521 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
     5581 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
     5641 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
     5701 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
     5761 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
     5821 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
     5881 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
     5941 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
     6001 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
     6061 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
     6121 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
     6181 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
     6241 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
     6301 cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     6361 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     6421 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     6481 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     6541 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     6601 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     6661 tacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     6721 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     6781 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     6841 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     6901 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     6961 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     7021 atgaccccga agcagggtta tgcagcggaa aagatccgtc gaccctttcc gacgctcacc
     7081 gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa
     7141 acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa
     7201 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     7261 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     7321 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     7381 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     7441 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     7501 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     7561 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     7621 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     7681 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     7741 ggccgcgaac ggcctcaccc caaaaatggc agccaagctg accacttctg cgctcggccc
     7801 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7861 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7921 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7981 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8041 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8101 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8161 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8221 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8281 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8341 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8401 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8461 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8521 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8581 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8641 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8701 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8761 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8821 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8881 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8941 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9001 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9061 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9121 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctagcttccc
     9181 gcttggtcgg gccgcttcgc gagggcccgt tgacgacaac ggtgcgatgg gtcccggccc
     9241 cggtcaagac gatgccaata cgttgcgaca ctacgccttg gcacttttag aattgcctta
     9301 tcgtcctgat aagaaatgtc cgaccagcta aagacatcgc gtccaatcaa agcctagaaa
     9361 atataggcga agggacgcta ataagtcttt cataagaccg cgcaaatcta aaaatatcct
     9421 tagattcacg atgcggcact tcggatgact tccgagcgag cctggaacct cagaaaaacg
     9481 tctgagagat accgcggatc tcacacagga aatttc
//
