LOCUS       sequence-317431-        9522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018367
FEATURES             Location/Qualifiers
     source          1..9522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2226..2246
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2247..2270
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2296..2352
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2353..2370)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2353..2369)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2362..2384)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2578..2597)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2697..2719
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2757..2775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3561..4760
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4991..6139)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6411..6782)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6815..6924
                     /note="incP origin of transfer"
     rep_origin      7077..7708
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8171..8759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8660..8679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8913..8930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9047..9068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9083..9088,9089..9106,9107..9113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9121..9137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9126..9148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9145..9161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9145..9161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaagc
      361 gcagggcgta ccaccggctg ctcttccttc tcatcctctc agccgtctcg tgggtcggcc
      421 tgtcgatcgc caacccccag gtcgaagaac tgtcgaagaa ggccggcctc tggcccgccc
      481 ccggccggga ctaccacctc acgcgctaca gcccgctgag ccagatcaat acgcataacg
      541 tgaagaacct cgacctggcc tggagcttct cgaccggcgt cctccgcggc cacgaaggcc
      601 agccgctcgt gatcggcaac acgatgtatg tgcagaccgc ctatcccaac atcgtctatg
      661 ccctggatat ctccaaggga aacgactaca agatcctgtg gaagtacgtc ccgcaacagg
      721 acgaccgcgc ggtcgctgtg gcctgctgcg ataccgtcat gcggggaatc tcttacgccg
      781 acggcaagat agtcatgaac acgctggatg gccacgtcat cgcactcgac gcgaagacag
      841 gcaaggagct gtggaaggtc aagcacgccg acccgtacaa gggcgaaacc atcacgatgg
      901 cgcccatcgt ggccaaagac aaggtcatcg tcggcatctc gggcggcgag ttcggcgtcc
      961 gggggcgtgt cgcggcgtac gatcttaact ccggccgccg cgtctggctg tgctacaacc
     1021 agggcccgga ccaggacatg tgctacggct cgcgcacccc ggccagctac aagggactgg
     1081 gcgtcaacac ctggcagggc gatcagtgga agatcggcgg ggccaccgtg tggggctggt
     1141 tctcctacga ccccaagctc aacctggtct actacggcac gtcgaacccg ggcacgtgga
     1201 acccctatat ccggtgttcc gcctcctggg aggaatgcca gtcgggccgc tacgacaaca
     1261 agtggagcac ctcgatcatc gcccgcgatg ccgacaccgg cgagttggtc tgggcgttcc
     1321 agatgacccc gttcgaccag tgggattacg acggcgtcaa cgagtccatc ctgctcgatg
     1381 tgaacgtcgg cagtaagaag gtgccagcgc tgattcactt cgaccgcaac ggcttcgcct
     1441 tcctcctcaa ccgcgccacg ggcgagctga tctacgctaa gccgttcgtg tacgtcaact
     1501 gggcgaagtc gtgggatgag cggcacgcgc gcgacggtca cactttcaag gtcacgaagg
     1561 accccacgaa aagcccgtgg aaaccgggcg acaacaagaa ggacgtctgc ccctccgcga
     1621 tgggcggcaa ggaccagcag ccggcggcgt acagcccgga aaccggcctg gtctatgtgc
     1681 cgaccaacca gtggtgcatg gactacgagc cagccgagac caagtacacc gagggcgccc
     1741 cctacgtcgg cgcgaatgtg cttatgaagt tttacgagca caagtcgtcg gtccacggcc
     1801 tgtccaagac cccgggccac tacggtcgca tcatagcctg ggatccggcg aagggcgaga
     1861 tcaagtgtta catcgacgag aagtacccga cctggagcgg cgtccttgtt accaagggcg
     1921 gcgtcggctt ctacggcacg atggacggtt ggttccgcgc gttcgacctg aagacctgca
     1981 aggtcctctg gtcgaagaag ctcggatcgg gcatcatcgg aaatccgatc tccttcgagg
     2041 tcaacggcaa gcagtacatc gcggtgtact ccggaatcgg gggctgggcg ggcctgcccg
     2101 tcaccgccaa cctggacccg aacgacccgt atggcggcct gggcgccacg aacctctccc
     2161 tcgagctgga tctctaccgg caggccacga tgtccggcgt cctgtacgtg ttttcgctgg
     2221 aggccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagctat
     2281 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     2341 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2401 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2461 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     2521 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2581 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2641 gccctgacgg gcttgtatgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     2701 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2761 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agcacccttt
     2821 ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc
     2881 gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc
     2941 cgcaacagcg gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg
     3001 tcgccggcct gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca
     3061 ttgacggcgt ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc
     3121 gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag
     3181 gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg
     3241 tcggctctcg gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg
     3301 agcagcgccc gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt
     3361 tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg
     3421 gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac
     3481 ataatatgtc caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga
     3541 ggctggtccg gaggccagac gtgaaaccca acatacccct gatcgtaatt ctgagcactg
     3601 tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg
     3661 atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt
     3721 tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc
     3781 caatcttgct cgtctcgctg gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc
     3841 ctttcctttg ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg
     3901 cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg
     3961 gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga
     4021 tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt
     4081 tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc
     4141 gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg
     4201 ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg
     4261 tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg
     4321 catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc
     4381 tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc
     4441 ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     4501 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     4561 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     4621 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     4681 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag
     4741 ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg gtcaaggcga
     4801 cttccggcaa gctatacgcg ccctagaatt gtcaatttta atcctctgtt tatcggcagt
     4861 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     4921 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     4981 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     5041 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     5101 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     5161 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     5221 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     5281 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     5341 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     5401 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     5461 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     5521 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     5581 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     5641 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     5701 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     5761 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     5821 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     5881 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     5941 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     6001 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     6061 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     6121 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     6181 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     6241 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     6301 tcttgccctg cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga
     6361 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
     6421 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
     6481 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
     6541 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
     6601 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
     6661 taggcctaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
     6721 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
     6781 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
     6841 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     6901 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     6961 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     7021 gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtcgacc ctttccgacg
     7081 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     7141 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
     7201 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
     7261 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
     7321 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
     7381 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
     7441 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
     7501 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
     7561 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
     7621 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
     7681 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
     7741 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcc aagctgacca cttctgcgct
     7801 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7861 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7921 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7981 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8281 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8881 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8941 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9001 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9061 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9121 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctag
     9181 cttcccgctt ggtcgggccg cttcgcgagg gcccgttgac gacaacggtg cgatgggtcc
     9241 cggccccggt caagacgatg ccaatacgtt gcgacactac gccttggcac ttttagaatt
     9301 gccttatcgt cctgataaga aatgtccgac cagctaaaga catcgcgtcc aatcaaagcc
     9361 tagaaaatat aggcgaaggg acgctaataa gtctttcata agaccgcgca aatctaaaaa
     9421 tatccttaga ttcacgatgc ggcacttcgg atgacttccg agcgagcctg gaacctcaga
     9481 aaaacgtctg agagataccg cggatctcac acaggaaatt tc
//
