LOCUS       sequence-317433-        9531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018371
FEATURES             Location/Qualifiers
     source          1..9531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2235..2255
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2256..2279
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2305..2361
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2362..2378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2371..2393)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2587..2606)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2706..2728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2766..2784)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3570..4769
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(5000..6148)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6420..6791)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6824..6933
                     /note="incP origin of transfer"
     rep_origin      7086..7717
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8180..8768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8669..8688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8922..8939
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9056..9077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9092..9097,9098..9115,9116..9122)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9130..9146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9135..9157
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9154..9170
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9154..9170
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaagg
      361 ggatgctcca cccggcccgc gcacgggtcg acgcgggcca gggcgcccgc gccctcgcca
      421 cagagaacag cttccgcacc cgcatacttc cggtgatgct gctcgcctgc ggcctcttcc
      481 tgtccggcgc gagcgaaccg gccagcgctg cggccatcgc ccccccggcc gcggccgccc
      541 cgcccgatga cggccagtgg acgatgcccg cgaagaatta tgcctccacc cgcttctcgg
      601 agctgtcgga aatcaacgag ggcaatgtca aaaacctgca ggtggccttc accttctcca
      661 ccggcgtcaa caagggtcag gaggccgcac ccttggtcgt cggcaacaat atgtacatcg
      721 tcaccccctt cccgaacatc gtctacgccc tcgacctctc gcgcccgggc gcgcccatga
      781 agtggaagta cgaaccgaac cccgagcctg ccgcgcaggg tgtggcgtgc tgcgacgtgg
      841 tgaatcgagg cgccgccttc gcggacggcc gtatcttttt caacaccctg gatggccaca
      901 ccatcgcgct cgatgccaac accgggaagc cgatctggaa cacccacatc gggaacatta
      961 acatcgggga gaccatcacc atggcgccgc tggtggtcaa gggcaaggtc ctcgttggca
     1021 acagcggcgg cgagatgggg gtccgcggtt gggtcaaagc gctcgatgcc ggagacggtc
     1081 acgtcgtctg gaccgcttac ggaaccggcc ccgacaagga cgtcctcatc ggccccgact
     1141 tcaagccgca ctacgatatg gaccggggca ccgacctggg cgtcacgacc tggccgccgg
     1201 aggcctggaa gatcggcggc gggaatatgt ggggctggat cagctacgac ccggatctga
     1261 acctcatctt ccacggtacg ggcaacccgg gcccgtggaa cccggacctc cgcccgggcg
     1321 acaataagtg gacctcgggc atcttcgcgc gcgacgccga caccggcgcg gctaagtggt
     1381 tctaccaatg gtctccccac gacctgcacg actacgacgg catcaacgag caaatcctgc
     1441 tcgatatgac gtggcagggt aagccgcgga aggtgctggt gcgccctgaa cggaacggct
     1501 acctctacat cctggaccgc accacggggg aggtcctctc ggcgacggct tacggtccgg
     1561 tgaactcgtc gaagggcgtc gacttgaaga cgggtcgcct gatcgcgaat cccgacaagc
     1621 gcacaggtac cggcaaagta gtgcgagaca tctgccctac cgcctcgggc ctgaaggact
     1681 ggcagccgag cgcgttctcc ccacgcaccg gcctgctcta catcccccat aacaacctct
     1741 gcatggacga ggagggcgtg gaggtcaact acatcgccgg taccccgtac gtcggcatga
     1801 acgtgcggat gatcccgggc ccgggcggca accgtggcgc gttcacggcg tgggacgtgg
     1861 cggcggcgaa gcccgcgtgg gtcctgaagg agaacttccc ggtatggagc ggcaccgtgg
     1921 tgaccgcggg caacgtggtg ttctacggta cgatggaagg ttggttcaaa gccgtcagcg
     1981 cgcgcaccgg cgacctcctc tggcaattca agaccagctc gggcatcatc ggacaaccga
     2041 tcacctaccg tggcccggac ggccatcagt acgtcgcgat cctgagcggc gtcggcgggt
     2101 gggcgggcgc tatcgtctcc ggtgacctcg acccccgaga tgccacggcc gccctgggct
     2161 tcgtgaacgc catgaaggac ctgaagaacg caaccacggc cggcggcacc ctgtacgtgt
     2221 tccgcttgcc ggccgaaaac ctctattttc agggacatca tcaccatcac catcaccact
     2281 gacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc
     2341 ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     2401 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     2461 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     2521 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     2581 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     2641 cgctgacgcg ccctgacggg cttgtatgct cccggcatcc gcttacagac aagctgtgac
     2701 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     2761 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2821 gcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg
     2881 acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct
     2941 atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg
     3001 gcatcgctgt cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc
     3061 atcagcgcat tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc
     3121 gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca
     3181 tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc
     3241 cagtcgtcgt cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc
     3301 agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc
     3361 cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg
     3421 tccagggcgg cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca
     3481 ctgataaaca taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg
     3541 accagcggag gctggtccgg aggccagacg tgaaacccaa catacccctg atcgtaattc
     3601 tgagcactgt cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc
     3661 tcctgcgcga tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc
     3721 tgtatgcgtt ggtgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg
     3781 ggcggcggcc aatcttgctc gtctcgctgg ccggcgccac tgtcgactac gccatcatgg
     3841 cgacagcgcc tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg
     3901 cgactggggc ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc
     3961 ggcacttcgg cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg
     4021 gtgggctgat gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg
     4081 gcctcaattt cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc
     4141 cgttacgccg ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg
     4201 tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg
     4261 cgctttgggt cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt
     4321 cgcttgccgc atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag
     4381 ccgcccggct cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct
     4441 acatcctgct tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg
     4501 cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg
     4561 aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg
     4621 gacccctcct cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat
     4681 ggattgcagg cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga
     4741 gcggcgcagg gcaacgagcc gatcgctgat cgtggaaacg ataggcctat gccatgcggg
     4801 tcaaggcgac ttccggcaag ctatacgcgc cctagaattg tcaattttaa tcctctgttt
     4861 atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc
     4921 gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg
     4981 gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt
     5041 gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact
     5101 tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt
     5161 acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc
     5221 ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct
     5281 cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt
     5341 gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg
     5401 cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc
     5461 ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct
     5521 tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc
     5581 cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct
     5641 cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca
     5701 tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga
     5761 tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca
     5821 ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca
     5881 tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg
     5941 atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag
     6001 cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc
     6061 ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt
     6121 atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg
     6181 ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat
     6241 ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg
     6301 tattccgaat cttgccctgc acgaatacca gctccgcgaa gtcgctcttc ttgatggagc
     6361 gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt
     6421 catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc
     6481 tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg
     6541 tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg
     6601 gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg
     6661 gccagcaggt aggcctacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt
     6721 cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt
     6781 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
     6841 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
     6901 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
     6961 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc
     7021 gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagat ccgtcgaccc
     7081 tttccgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt ctatggccct
     7141 gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg ccggcgttgt
     7201 ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga
     7261 ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg
     7321 acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca
     7381 cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg
     7441 actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga
     7501 ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag
     7561 ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata
     7621 tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag
     7681 gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc catcccccca
     7741 ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcca agctgaccac
     7801 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7861 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7921 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7981 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8041 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8101 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8161 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8221 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8281 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8341 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8401 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8461 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8521 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8581 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8641 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8701 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8761 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8821 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8881 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8941 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9001 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9061 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9121 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9181 ccaagctagc ttcccgcttg gtcgggccgc ttcgcgaggg cccgttgacg acaacggtgc
     9241 gatgggtccc ggccccggtc aagacgatgc caatacgttg cgacactacg ccttggcact
     9301 tttagaattg ccttatcgtc ctgataagaa atgtccgacc agctaaagac atcgcgtcca
     9361 atcaaagcct agaaaatata ggcgaaggga cgctaataag tctttcataa gaccgcgcaa
     9421 atctaaaaat atccttagat tcacgatgcg gcacttcgga tgacttccga gcgagcctgg
     9481 aacctcagaa aaacgtctga gagataccgc ggatctcaca caggaaattt c
//
