LOCUS       sequence-317436-        9537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018373
FEATURES             Location/Qualifiers
     source          1..9537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2241..2261
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2262..2285
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2311..2367
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2368..2385)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2368..2384)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2377..2399)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2593..2612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2712..2734
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2772..2790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3576..4775
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(5006..6154)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6426..6797)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6830..6939
                     /note="incP origin of transfer"
     rep_origin      7092..7723
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8186..8774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8675..8694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8928..8945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9062..9083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9098..9103,9104..9121,9122..9128)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9136..9152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9141..9163
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9160..9176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9160..9176
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgcggg
      361 agaagaagct ctcgcgcctg acgaagaccg ggtcggcggc cctcgtctcc tccgtaatcg
      421 ggatgatgac cgtcggggcc gcgttcgcca acgacaagct cgtcgaactc cagaaggacg
      481 aggcgaactg ggtcatgcag ggcaagaact acagcgcgga ccactactcg tcctcgaagc
      541 agatcaacaa gaagaatgtg tcgaagcttg caccgtcgtg gaccttttcg acgggggtcc
      601 tgtcgggcca cgagggcgcc ccactcgtga taggggatat gatgtacatc cacagcccgt
      661 tccccaacaa caccttcgcg atcaacctga acgatccggg cacgatcgtg tgggagcata
      721 agcccaagca ggaccccgcc gcgcgcgcgg tcgcctgttg cgacgtcgtc aaccgcggcc
      781 tggcctactg gccgggcgat gataagacgc cggccatgat cgtcaagagc cagctggacg
      841 ggcacgtcgt cgcgctcaac gccgagaccg gggaactcca ctggcaggtg gagaatgggg
      901 acatctcggt cggccagacg cagacccagg cccccttcgt ggtcaaggac ctcgtcatcc
      961 aaggctcctc gggcgcggag ctgggtgtgc ggggctacgt caccgcgttc gacatccata
     1021 ccggcgagat ggcctggcgc tggtatgcca cgggtcccga ttcggaaatc gggctcgcca
     1081 aggacttcaa cagcgaaaac ccgcactacg gccagaagga cctcggcctc aagacctggg
     1141 aggacgaggc ctggaagatc ggcgggggca ccaactgggg ctggtatgcg tacgacccgg
     1201 acctcgagat gttctactac ggcagcggca acccggcccc ctggaacgag accatgcgcc
     1261 cgggcgacaa caagtggacc atgaccatct gggggcgcga cgccgatacg ggccttgcca
     1321 agttcggcta ccaaaagacg ccccacgacg agtgggatta cgcgggcgtg aacgtcatga
     1381 tgctgagcga acagaaggac aagagcggga agatgcgtaa gctgctgacg cacccggatc
     1441 gcaatggcat catctacacg ctcgaccgcg agacgggcga cctggtctcc gcggacaaga
     1501 tggacgatac cgccaactgg gtgaagaaag tggacctgga gaccggcctg ccgatccgcg
     1561 acccggagtt ctcgacgcgc atggggcacc ggtcgcgtga catctgcccg tcggcgatgg
     1621 gcttccacaa ccagggcatg gacagctacg accccgagcg ccagaatttc tacctcggga
     1681 tcaatcacct ctgcatggac tgggagccct tcatgctgcc gtaccgtgcg ggacagttct
     1741 tcgtgggcgc caacgtgtgg tcgtacccgg gccccaaggg cgaccggcag cgcgggatcg
     1801 ggtcgggcca ggtcaaagcc tataacgcca tcaccggcga gttcgcgtgg gaaaagatgg
     1861 agaagttctc aatctggggc ggcacgatgg ccacggccgg cggcctcgtg ttctacggca
     1921 ccttggacgg ttttatcaaa gcgcgggacg cggacaacgg ggacctgctc tggaagttca
     1981 agctgccatc cggcgcgatc gggcatccga tgacctacac caaggacggc gtacagtacg
     2041 tcgccatcaa ctacggggtt ggcggctggc cggccgtggg cctcgtgttc gatctcaacg
     2101 acccctccgc gggcttgggt gcggtgggcg ccttcaagga gctggcccgt aacacgcaga
     2161 tgggcggtgg cttgatggtc ttcgccctgg acggcaaggg cccgtacgat gacctcagcg
     2221 tgggagagta tgaaatggcc gaaaacctct attttcaggg acatcatcac catcaccatc
     2281 accactgaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt cgactctaga
     2341 ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga
     2401 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2461 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2521 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     2581 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2641 aacacccgct gacgcgccct gacgggcttg tatgctcccg gcatccgctt acagacaagc
     2701 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2761 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2821 ttcttagcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat
     2881 ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag
     2941 gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc
     3001 tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg
     3061 attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga
     3121 agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc
     3181 gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat
     3241 gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct
     3301 tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc
     3361 tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc
     3421 aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact
     3481 ttatcactga taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc
     3541 aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacata cccctgatcg
     3601 taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc
     3661 cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc
     3721 tggcgctgta tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc
     3781 gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg cgccactgtc gactacgcca
     3841 tcatggcgac agcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca
     3901 ccggggcgac tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc
     3961 gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg
     4021 tgctcggtgg gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct
     4081 tgaacggcct caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac
     4141 gccggccgtt acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca
     4201 tgaccgtcgt cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc
     4261 cggccgcgct ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg
     4321 gcatttcgct tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc
     4381 ctgtagccgc ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca
     4441 caggctacat cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc
     4501 tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg
     4561 atgaggaacg tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga
     4621 tcgtcggacc cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt
     4681 gggcatggat tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc
     4741 tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag gcctatgcca
     4801 tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta gaattgtcaa ttttaatcct
     4861 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
     4921 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
     4981 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     5041 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     5101 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     5161 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     5221 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     5281 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     5341 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     5401 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     5461 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     5521 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     5581 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     5641 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     5701 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     5761 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     5821 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     5881 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
     5941 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     6001 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     6061 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     6121 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     6181 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     6241 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     6301 acttggtatt ccgaatcttg ccctgcacga ataccagctc cgcgaagtcg ctcttcttga
     6361 tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa
     6421 aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc
     6481 tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
     6541 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
     6601 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
     6661 ccgacggcca gcaggtaggc ctacaggctc atgccggccg ccgccgcctt ttcctcaatc
     6721 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
     6781 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
     6841 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
     6901 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
     6961 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
     7021 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt
     7081 cgaccctttc cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
     7141 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
     7201 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     7261 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     7321 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
     7381 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     7441 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     7501 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     7561 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     7621 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     7681 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     7741 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagccaagct
     7801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8941 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9001 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9061 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9181 attacgccaa gctagcttcc cgcttggtcg ggccgcttcg cgagggcccg ttgacgacaa
     9241 cggtgcgatg ggtcccggcc ccggtcaaga cgatgccaat acgttgcgac actacgcctt
     9301 ggcactttta gaattgcctt atcgtcctga taagaaatgt ccgaccagct aaagacatcg
     9361 cgtccaatca aagcctagaa aatataggcg aagggacgct aataagtctt tcataagacc
     9421 gcgcaaatct aaaaatatcc ttagattcac gatgcggcac ttcggatgac ttccgagcga
     9481 gcctggaacc tcagaaaaac gtctgagaga taccgcggat ctcacacagg aaatttc
//
