LOCUS       sequence-31744-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Leung JY, Kolligs FT, Wu R, Zhai Y, Kuick R, Hanash S, Cho KR, 
            Fearon ER
  TITLE     Activation of AXIN2 expression by beta-catenin-T cell factor. A 
            feedback repressor pathway regulating Wnt signaling.
  JOURNAL   J Biol Chem. 2002 Jun 14. 277(24):21657-65.
  PUBMED    11940574
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Jun 14. 277(24):21657-65."
COMMENT     Sequence Label: 200 bp Axin2 mutant
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    322..413
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(354..373)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    427..475
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(743..764)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttacttaga tcgcagatct cgagcccggg ctagccaagt cagcaggggc
      121 tcatctgaac ctcctctctg gaaagaaaag gaagggggga ggtggggaga gagaaaaggg
      181 tattgatcta atcaaaatca gatctaccca acgccaaagc aagaaagtaa acaggctttt
      241 caactcctca aattcaaatc aagcaaccca gctatctgcg aggtgctcat ctaaagtatc
      301 aggcgctggg tacctatcga tagagaaatg ttctggcacc tgcacttgca ctggggacag
      361 cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta
      421 tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattgcggc
      481 cgctccaagt acctcccgta ccttaatatt acttacttat catggtagct tgggctggcg
      541 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      601 atggcaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      661 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      721 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      781 ctccaacgtc aaagggcgaa aaaccgtcta tcanggcgat ggcccactac gtgaaccatc
      841 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgag
//
