LOCUS       sequence-317440-        9567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018385
FEATURES             Location/Qualifiers
     source          1..9567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2271..2291
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2292..2315
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2341..2397
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2398..2415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2398..2414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2407..2429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2623..2642)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2742..2764
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2802..2820)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3606..4805
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(5036..6184)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6456..6827)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6860..6969
                     /note="incP origin of transfer"
     rep_origin      7122..7753
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8216..8804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8705..8724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8958..8975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9092..9113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9128..9133,9134..9151,9152..9158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9166..9182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9171..9193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9190..9206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9190..9206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaagc
      361 tcagcctcgg cgttctgacg ggcacgatcg tcgcggtcac gatgggcctc gcgggtcagg
      421 cctccgccat gaacaccgac gaggagcagc tcaagcgcat ggccgacccg gaccaatggc
      481 ccgccccggg ccgcgacttc gccctgaccc gccatagctc gctgtccgac atctcgaccg
      541 aaaacgtcaa caaactgcag atggcgtgga tgcagggcac gaacgcgctg cgtggccatg
      601 agggccagcc gctggtcatc aaggacgtgg gcggcaagcc catgctcttc atgatcagcg
      661 gctgcccctc gatggccaac tgcaatgtgg tccagggcct ggacttgtcg gatccggaca
      721 acccgaaaca ggtctggagc tatgttaaga agaccgaccg cgacgagtcg gctgtgccgc
      781 gggcctgctg cgataccgtc aaccgcggcg gcagctacgc ggatggcaag ttcgtcttcg
      841 gcaccctgga cgggttcctg atcgcactgg acggccagac cggcaaggaa gtgtgggtgg
      901 tgaagtgggc gtaccccgag aagggcgaga ccatcacccc ggccccgctg atcgcggaca
      961 acaaggtttt catcggcttc ggcgggtcgg agttcgcggc gcgcggccgc gtcggcgcgt
     1021 tcaacctcgc tgacggcagc gaggcctggg tctgccacac cacggggccg gataaggacg
     1081 tgtgtctcac cccggagacc aacaaggcca atccccacta cgggaccgcc ggccaagacc
     1141 tcggcgtcaa gaccttccca aacgaggact ggaagatcgg cggcgggacc gcctggggtt
     1201 ggtactcgta cgacccggac acgaagttgg tctactactc gaccgcgaac cccggcctgt
     1261 ggtcgcccca gtatcgctgc ccggacaaga cccacgagga gtgcaacgcc gtcgacccct
     1321 ccctggacgg gcaggtcggc cgcttcgaca acaaatggac gctctccatc ttcgcccgca
     1381 aaatcgagaa cggcgaggcc gtctgggtgt atcagatcac ccccttcgac cagtgggact
     1441 acgacggcat caacgagaac atcatcacgg aaatggaggt cgatggcaag aagcgaaagg
     1501 tcctcagcca cttcgatcgc aacggcttcg cctatgtttg ggacgccgcg gacggcaccg
     1561 tcctgcgcgc caccaagtat gtgacgaccg actgggccga gaagatcgat ctcaagacgg
     1621 gacgacccat caaggtccgg gatcattcgc cgctcgaggt cggccgcaat gtcagcgcct
     1681 gccccagcgc gatgggcggc aaggaccagc agccggccag cgttgaccca aaggagccga
     1741 acctcttcta cgtcccgacc aacaactggt gcatggagct ggagccgcag gagcgtacgc
     1801 atacgaacca aggcacggtc tacgtcttcg ccaacgtgta tatgtacccg gagaagcagg
     1861 gcgttaccgg gaagatcaag aagtacgacg tcctgacggg caagaccctc tgggaaatcc
     1921 ccgacccctt cccgaactgg tcgggcacca tgaacaccga cggcgggctg atgttctacg
     1981 gctcgctcgg cggggacttc cgcgcggtcg atcgggatac cggcaagatc ctctggagca
     2041 ggaagctggg gtcgggcatc atcgggaacc ccattacctt caagacgggc gggaagcagt
     2101 acgtcggggt ctacagcggc atcggcggct ggatcgggct gccggtgacc gccgggctgg
     2161 acctcaatga caagttcggc gcgataggcg ccaccgcgat gaccaaggcc gccggcctcc
     2221 agcacgtccc gcagggtggc accctgaacg tcttccgggt ctacgacgcc gaaaacctct
     2281 attttcaggg acatcatcac catcaccatc accactgaca gctatgacca tgattacgcc
     2341 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
     2401 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     2461 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     2521 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     2581 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     2641 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2701 tatgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2761 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2821 tttataggtt aatgtcatga taataatggt ttcttagcac cctttctcgg tccttcaacg
     2881 ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg
     2941 aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag
     3001 agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc
     3061 tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg
     3121 gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt
     3181 gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc
     3241 ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc
     3301 gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg
     3361 ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt
     3421 gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta
     3481 ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat atgtccacca
     3541 acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc
     3601 cagacgtgaa acccaacata cccctgatcg taattctgag cactgtcgcg ctcgacgctg
     3661 tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga
     3721 acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttggtg caatttgcct
     3781 gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct
     3841 cgctggccgg cgccactgtc gactacgcca tcatggcgac agcgcctttc ctttgggttc
     3901 tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt
     3961 atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct
     4021 gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc
     4081 cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt
     4141 tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc
     4201 cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct
     4261 tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg
     4321 atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc
     4381 attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg
     4441 cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac
     4501 ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg
     4561 cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct
     4621 cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct
     4681 atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact
     4741 tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc
     4801 gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat
     4861 acgcgcccta gaattgtcaa ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg
     4921 tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa
     4981 atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc
     5041 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
     5101 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
     5161 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
     5221 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
     5281 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
     5341 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
     5401 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
     5461 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
     5521 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
     5581 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
     5641 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
     5701 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
     5761 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
     5821 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
     5881 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
     5941 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
     6001 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg
     6061 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
     6121 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
     6181 tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct
     6241 gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg
     6301 tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga
     6361 ataccagctc cgcgaagtcg ctcttcttga tggagcgcat ggggacgtgc ttggcaatca
     6421 cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg gctctgccct cgggcggacc
     6481 acgcccatca tgaccttgcc aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg
     6541 aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc
     6601 aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac gtgctcatag
     6661 tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc ctacaggctc
     6721 atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc
     6781 ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca
     6841 tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc
     6901 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
     6961 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
     7021 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
     7081 aagcagggtt atgcagcgga aaagatccgt cgaccctttc cgacgctcac cgggctggtt
     7141 gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg
     7201 aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc
     7261 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg
     7321 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa
     7381 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg
     7441 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga
     7501 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag
     7561 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac
     7621 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag
     7681 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc
     7741 ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa
     7801 cggcctcacc ccaaaaatgg cagccaagct gaccacttct gcgctcggcc cttccggctg
     7861 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7921 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7981 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8041 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8101 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8161 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8221 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8281 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8341 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8401 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8461 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8521 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8581 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8641 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8701 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8761 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8821 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8881 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8941 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9001 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9061 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9181 aatttcacac aggaaacagc tatgaccatg attacgccaa gctagcttcc cgcttggtcg
     9241 ggccgcttcg cgagggcccg ttgacgacaa cggtgcgatg ggtcccggcc ccggtcaaga
     9301 cgatgccaat acgttgcgac actacgcctt ggcactttta gaattgcctt atcgtcctga
     9361 taagaaatgt ccgaccagct aaagacatcg cgtccaatca aagcctagaa aatataggcg
     9421 aagggacgct aataagtctt tcataagacc gcgcaaatct aaaaatatcc ttagattcac
     9481 gatgcggcac ttcggatgac ttccgagcga gcctggaacc tcagaaaaac gtctgagaga
     9541 taccgcggat ctcacacagg aaatttc
//
