LOCUS       sequence-317442-        9576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018387
FEATURES             Location/Qualifiers
     source          1..9576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2280..2300
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2301..2324
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2350..2406
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2407..2424)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2407..2423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2416..2438)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2632..2651)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2751..2773
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2811..2829)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3615..4814
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(5045..6193)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6465..6836)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6869..6978
                     /note="incP origin of transfer"
     rep_origin      7131..7762
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8225..8813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8714..8733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8967..8984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9101..9122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9137..9142,9143..9160,9161..9167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9175..9191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9180..9202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9199..9215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9199..9215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accatgaagt
      361 tcgagaagct gggcttgggt atcggtctca cggccgcgct gctcctcgcc ccggtgtccg
      421 tgatggcgga ccagtccgcg tccgccgaaa tcgacgagcg cgccaaggac cccgacctgt
      481 ggccggcccc cggccgcgat aaccagctca cgcggcactc cccgctcaag gacatcaaca
      541 ccgaaaacgt gggcaagctg ggcatgatct gggcccagtc catgaactcg ctccgcggcc
      601 aagaggggca gcccatcgtg atcaagctcg aggatggaac cccgatgatg tacttcgtct
      661 cgggctgccc gtcgatgccc ctgtgcaatg tcgtgcaagc cctggatctg acagaccccg
      721 ataacccgaa gcagatctgg aactacgtca agaagacgga ccgggacgag tccgccattc
      781 cgcgcgcctg ctgcgacacc gtgaaccgcg gcctgaatta cgctaagggt aaggtggtct
      841 tccacaccct cgacggcttc gtgatcgcgc tggacggcaa gaccggcaag gaaatctgga
      901 ccgtgaagca cgcctacccc gagcacggcg agacccacac gggcccgacc atgatcgccg
      961 agaacctcgt cctcgcaggc ttcggcggtg acgaattcgc cgcacgcggc cgcatcacgg
     1021 cctacaacat cgaggacggc agcgaagtgt ggtcctgcca tagcaccggc tcggataagg
     1081 atgtgtgcct caccgccgac accaacaagg ccaaccccca ttacggctcg tacggcaagg
     1141 acctcggcgt cgtgacctac ccgaaggacg agtggaagat cggcggcggt tccccgtggg
     1201 cctggttctc ttacgatccg gagctcaaga tgatctacta ctccaccgga aacccaggcc
     1261 actggtcgcc gggcctgcga tgcggactgg aaggcgaaga ccacacccac gagaagtgca
     1321 acgagttcga cccggacttg acctccggca aattcgacaa caagtggtcc atgacgatct
     1381 tcgggcggaa ggtggacacc ggcgaggctg tcttcgccta ccagatgacc cccttcgatc
     1441 agtgggacta cgacggagtc aacgagaaca tccttgtcaa catggaggtc gacggcaagg
     1501 accgcaaaac cctcgtccac ttcgatcgca atggcttcgc gtacgtgttg gaccgggtgg
     1561 acggcacctt gctgcgcgcg aacaagttcg tcacggtcga ttgggcggag aaggtggacc
     1621 tcaagaccgg ccgccctatc aaggtcaagg agcactcccc cttcgatatc ggcgtcaaca
     1681 cctccgcctg cccgtccgcc atgggcggga aggaccagca gccgtgcgcc atcgacccga
     1741 aggaaggcaa catcgcgtac tgccccacga acaattggtg catggaggat gagccgcagg
     1801 agcgcaccca cacccagcag ggcaccgtct acgtgttcgc caacgtctac atgtacccgg
     1861 agaagccggg cgttacaggc aagttcaaga aattcgacgt cctcaccgga aagaccctgt
     1921 gggagatccc cgacccgtat ccgaactggg gcggtgccgc ggtgaccgac ggaggcctgg
     1981 tgttctacgg ctcattgggc ggggacttcc gggcggtcga taggaagacg ggcaaggtcc
     2041 tgtggagccg gaagctcggc agcgggatca tcggcaaccc gatcacctac aagatcgatg
     2101 gcaagcagta tgtgagcgtc cattcgggca tcggcggctg gattggcctt cctgtcacag
     2161 ccggcctcga tatgagcgac aagttcggcg ccatcggcgc gaccgccatg gcgaaggcca
     2221 ccggcctgtc gaatatcccg cagggtggca ccgtcaccac gttccgcctg cttgacgccg
     2281 aaaacctcta ttttcaggga catcatcacc atcaccatca ccactgacag ctatgaccat
     2341 gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
     2401 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2461 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2521 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     2581 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     2641 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     2701 acgggcttgt atgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     2761 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     2821 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagcacc ctttctcggt
     2881 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
     2941 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
     3001 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
     3061 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     3121 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     3181 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
     3241 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     3301 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     3361 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     3421 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     3481 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     3541 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     3601 tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc actgtcgcgc
     3661 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     3721 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttggtgc
     3781 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct
     3841 tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca gcgcctttcc
     3901 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     3961 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     4021 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     4081 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     4141 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     4201 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     4261 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     4321 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     4381 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     4441 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     4501 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     4561 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     4621 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     4681 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     4741 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     4801 gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag gcgacttccg
     4861 gcaagctata cgcgccctag aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
     4921 agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt
     4981 gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa
     5041 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
     5101 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
     5161 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
     5221 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
     5281 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
     5341 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
     5401 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
     5461 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
     5521 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
     5581 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
     5641 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
     5701 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
     5761 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
     5821 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
     5881 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
     5941 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
     6001 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
     6061 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
     6121 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
     6181 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
     6241 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
     6301 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
     6361 cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     6421 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     6481 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     6541 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     6601 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     6661 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     6721 tacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     6781 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     6841 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     6901 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     6961 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     7021 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     7081 atgaccccga agcagggtta tgcagcggaa aagatccgtc gaccctttcc gacgctcacc
     7141 gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa
     7201 acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa
     7261 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     7321 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     7381 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     7441 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     7501 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     7561 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     7621 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     7681 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     7741 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     7801 ggccgcgaac ggcctcaccc caaaaatggc agccaagctg accacttctg cgctcggccc
     7861 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7921 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7981 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8041 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8341 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8881 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8941 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9001 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9061 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9181 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctagcttccc
     9241 gcttggtcgg gccgcttcgc gagggcccgt tgacgacaac ggtgcgatgg gtcccggccc
     9301 cggtcaagac gatgccaata cgttgcgaca ctacgccttg gcacttttag aattgcctta
     9361 tcgtcctgat aagaaatgtc cgaccagcta aagacatcgc gtccaatcaa agcctagaaa
     9421 atataggcga agggacgcta ataagtcttt cataagaccg cgcaaatcta aaaatatcct
     9481 tagattcacg atgcggcact tcggatgact tccgagcgag cctggaacct cagaaaaacg
     9541 tctgagagat accgcggatc tcacacagga aatttc
//
