LOCUS       sequence-31745-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Snail plasmid
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPGS-hSNAIL.fl.flag
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    28..580
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    30..596
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(190..209)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     419..436
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     606..625
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        606..624
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     649..665
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     650..666
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(759..782)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actgcgtgca tccatcttgt tcnaccatat tatcatcgtg tttttcaaag gaaaaccacg
       61 tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca tgtaaagcat
      121 gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc atccttcagc
      181 cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat ccaactcaca
      241 acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg cttttggccg
      301 cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc tacagacgtt
      361 gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa cagaccttgc
      421 attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac agggccaggt
      481 ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac
      541 gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg ggcggaattc
      601 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg
      661 gtatcgataa gcttgatatc gaattcctgc agcccggggg atccgcccgg gctagagcgg
      721 ccgcgaattc actagtgatc gtaggatcct catacgatct tgtcatcgtc gtccttgtag
      781 tcgcggggac atcctgagca gccggactct tggtgcttgt ggagcaggga cattcgggag
      841 aangtccgag cacacgcctg gcactggtac ttcttgacat ctga
//
