LOCUS       sequence-317457-         368 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose-inducible expression of supP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: pJH474_pTech_Para-supP
FEATURES             Location/Qualifiers
     source          1..368
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        118..283
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     206..225
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc
       61 tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa
      121 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat
      181 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat
      241 cctacctgac gctttttatc gcaactctct actgtttctc catgccgaag tggcgaaatc
      301 ggtagacgca gttgattcua aatcaaccgt agaaatacgt gccggttcga gtccggcctt
      361 cggcacca
//
