LOCUS       sequence-317458-        4019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose-inducible expression of six mutagenesis genes, each 
            encoding one amber codon after the initiator methionine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: 
            MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG
FEATURES             Location/Qualifiers
     source          1..4019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        118..283
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     206..225
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3103..3360
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="M*TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVH
                     TAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             3390..4019
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="M*TDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGE
                     RRACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEK
                     ILEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKI
                     FIQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc
       61 tcgctaaccc aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa
      121 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat
      181 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat
      241 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttggacg
      301 cgtacaactc aagtctgaca taaatgaccg ctatgtagag cactgcaatt acacgccaga
      361 tcgttctcgc taccgcaacc accggtatga accagattgg tgcgcactat gaaggccaca
      421 agatcattga gattggtgcc gttgaagtgg tgaaccgtcg cctgacgggc aataacttcc
      481 atgtttatct caaacccgat cggctggtgg atccggaagc ctttggcgta catggtattg
      541 ccgatgaatt tttgctcgat aagcccacgt ttgccgaagt agccgatgag ttcatggact
      601 atattcgcgg cgcggagttg gtgatccata acgcagcgtt cgatatcggc tttatggact
      661 acgagttttc gttgcttaag cgcgatattc cgaagaccaa tactttctgt aaggtcaccg
      721 atagccttgc ggtggcgagg aaaatgtttc ccggtaagcg caacagcctc gatgcgttat
      781 gtgctcgcta cgaaatagat aacagtaaac gaacgctgca cggggcatta ctcgatgccc
      841 agatccttgc ggaagtttat ctggcgatga ccggtggtca aacgtcgatg gcttttgcga
      901 tggaaggaga gacacaacag caacaaggtg aagcaacaat tcagcgcatt gtacgtcagg
      961 caagtaagtt acgcgttgtt tttgcgacag atgaagagat tgcagctcat gaagcccgtc
     1021 tcgatctggt gcagaagaaa ggcggaagtt gcctctggcg agcataattt aatatcagta
     1081 aaccggacat aacccatgta gaagaaaaat cgcgcttttt tgaagtgggc agggggcaag
     1141 tatcccctgc ttgatgatat taaacggcat ttgcccaagg gcgaatgtct ggttgagcct
     1201 tttgtaggtg ccgggtcggt gtttctcaac accgactttt ctcgttatat ccttgccgat
     1261 atcaatagcg acctgatcag tctctataac attgtgaaga tgcgtactga tgagtacgta
     1321 caggccgcac gcgagctgtt tgttcccgaa acaaattgcg ccgaggttta ctatcagttc
     1381 cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg cggtactgtt tttatatttg
     1441 aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc gcggtgagtt taacgtgccg
     1501 ttcggccgct acaaaaaacc ctatttcccg gaagcagagt tgtatcactt cgctgaaaaa
     1561 gcgcagaatg cctttttcta ttgtgagtct tacgccgata gcatggcgcg cgcagatgat
     1621 gcatccgtcg tctattgcga tccgccttat gcaccgctgt ctgcgaccgc caactttacg
     1681 gcgtatcaca caaacagttt tacgcttgaa caacaagcgc atctggcgga gatcgccgaa
     1741 ggtctggttg agcgccatat tccagtgctg atctccaatc acgatacgat gttaacgcgt
     1801 gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc gacgcagtat aagcagcaac
     1861 ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt acaaaccagg agtcgtttca
     1921 cccgcgaaaa aataattcag ctaagacact gcactggatt aagatgtaga aaacgattga
     1981 agttgatgat gaactctaca gctatattgc cagccacact aagcatatcg gcgagagcgc
     2041 atccgacatt ttacggcgta tgttgaaatt ttccgccgca tcacagcctg ctgctccggt
     2101 gacgaaagag gttcgcgttg cgtcacctgc tatcgtcgaa gcgaagccgg tcaaaacgat
     2161 taaagacaag gttcgcgcaa tgcgtgaact tctgctttcg gatgaatacg cagagcaaaa
     2221 gcgagcggtc aatcgcttta tgctgctgtt gtctacacta tattctcttg acgcccaggc
     2281 gtttgccgaa gcaacggaat cgttgcacgg tcgtacacgc gtttactttg cggcagatga
     2341 acaaacgctg ctgaaaaatg gtaatcagac caagccgaaa catgtgccag gcacgccgta
     2401 ttgggtgatc accaacacca acaccggccg taaatgcagc atgatcgaac acatcatgca
     2461 gtcgatgcaa ttcccggcgg aattgattga gaaggtttgc ggaactatct aacggctgaa
     2521 attaatgagg tcatacccaa atgtaggata gttcgtttac gcccattgaa caaatgctaa
     2581 aatttcgcgc cagccgccac gaagattttc cttatcagga gatccttctg actcgtcttt
     2641 gcatgcacat gcaaagcaag ctgctggaga accgcaataa aatgctgaag gctcagggaa
     2701 ttaacgagac gttgtttatg gcgttgatta cgctggagtc tcaggaaaac cacagtattc
     2761 agccttctga attaagttgt gctcttggat catcccgtac caacgcgacg cgtattgccg
     2821 atgaactgga aaaacgcggt tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc
     2881 tgcatctgca attaacggaa aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc
     2941 ataactgcct gcatcaactc tggtccgcgc tcagcacaac agaaaaagat cagctcgagc
     3001 aaatcacccg caaattgctc tcccgtctcg accagatgga acaagacggt gtggttctcg
     3061 aagcgatgag ctaataatac aaaaattagg aggaatttca acatgtagac aaatttatct
     3121 gacatcattg aaaaagaaac aggaaaacaa ctagtgattc aagaatcaat tctaatgtta
     3181 ccagaagaag tagaggaagt aattgggaat aaaccagaaa gtgatatttt agttcatact
     3241 gcttatgatg aaagtacaga tgaaaatgta atgctattaa cttcagatgc tccagaatat
     3301 aaaccttggg ctttagtaat tcaagacagt aatggagaaa ataaaattaa aatgttataa
     3361 gtcgagatta agtaaaccgg aatctgaaga tgtagaccga cgcggaatac gttcgtatcc
     3421 acgaaaaact ggacatctac accttcaaaa aacagttctt caacaacaaa aaatctgttt
     3481 ctcaccgttg ctacgttctg ttcgaactga aacgtcgtgg tgaacgtcgt gcgtgcttct
     3541 ggggttacgc ggttaacaaa ccgcagtctg gtaccgaacg tggtatccac gcggaaatct
     3601 tctctatccg taaagttgaa gaatacctgc gtgacaaccc gggtcagttc accatcaact
     3661 ggtactcttc ttggtctccg tgcgcggact gcgcggaaaa aatcctggaa tggtacaacc
     3721 aggaactgcg tggtaacggt cacaccctga aaatctgggc gtgcaaactg tactacgaaa
     3781 aaaacgcgcg taaccagatc ggtctgtgga acctgcgtga caacggtgtt ggtctgaacg
     3841 ttatggtttc tgaacactac cagtgctgcc gtaaaatctt catccagtct tctcacaacc
     3901 agctgaacga aaaccgttgg ctggaaaaaa ccctgaaacg tgcggaaaaa cgtcgttctg
     3961 aactgtctat catgatccag gttaaaatcc tgcacaccac caaatctccg gcggtttaa
//
