LOCUS       sequence-317459-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose-inducible expression of green fluorescent protein encoding
            zero amber codons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: pJH25_pBad_AraC_Para-sfGFP.0TAG
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             join(131..133,134..844)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             851..868
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
       61 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
      121 ttaacctagg atgagcaagg gcgaagaact gtttacgggc gtggtgccga ttctggtgga
      181 actggatggt gatgtcaatg gtcacaaatt cagcgtgcgc ggcgaaggtg aaggcgatgc
      241 aaccaatggt aaactgacgc tgaagtttat ttgcaccacg ggtaaactgc cggttccgtg
      301 gccgaccctg gtcaccacgc tgacgtatgg tgttcagtgt ttcagtcgtt acccggatca
      361 catgaaacgc cacgactttt tcaagtccgc gatgccggaa ggttatgtcc aagaacgtac
      421 catctcattt aaagatgacg gcacctacaa aacgcgcgcc gaagtgaaat tcgaaggtga
      481 tacgctggtt aaccgtattg aactgaaagg catcgatttt aaggaagacg gtaatattct
      541 gggccataaa ctggaatata acttcaattc gcacaacgtg tacatcaccg cagataagca
      601 gaagaacggt atcaaggcta acttcaagat ccgccataat gtggaagatg gcagcgttca
      661 actggccgac cactatcagc aaaacacccc gattggtgat ggcccggtcc tgctgccgga
      721 caatcattac ctgagcacgc agtctgtgct gagtaaagat ccgaacgaaa agcgtgacca
      781 catggtcctg ctggaattcg tgaccgcggc cggcatcacg cacggtatgg acgaactgta
      841 taaaggctca catcatcatc atcatcattg actcgagatc tgcagctggt accatatggg
      901 aattcgaagc ttggctgttt tggcggatga gagaagattt tcagcc
//
