LOCUS       sequence-317463-        1249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lactate-inducible expression of green fluorescent protein encoding 
            zero amber codons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: pJH625_pJH_LldR_PlldR-sfGFP.0TAG
FEATURES             Location/Qualifiers
     source          1..1249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      20..47
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             383..1096
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
       61 actctgctag caagtaaggc cgaccaacaa cctaagggca attctctgat gaggattgcc
      121 cttttcttta ccagacatct ccccccacaa gaattggccc taccaattct tcgcttatct
      181 gacctctggt tcacaatttc ccaattaaaa ctcacatcaa tgttgccaat acataacatt
      241 tagttaacca ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac
      301 aacgtgtgac aaggagatga gcaacagact cattacacga tgtgcgtgga ctcccattta
      361 actttaagaa ggagatatac atatgcgtaa aggtgaagaa ctgttcaccg gtgttgttcc
      421 gatcctggtt gaactggacg gtgacgttaa cggtcacaaa ttctctgttc gtggtgaagg
      481 tgaaggtgac gctaccaacg gtaaactgac cctgaaattc atctgcacca ccggtaaact
      541 gccggttccg tggccgaccc tggttaccac cctgacctac ggtgttcagt gcttcgctcg
      601 ttacccggac cacatgaaac agcacgactt cttcaaatct gctatgccgg aaggttacgt
      661 tcaggaacgt accatctctt tcaaagacga cggtacctac aaaacccgtg ctgaagttaa
      721 attcgaaggt gacaccctgg ttaaccgtat cgaactgaaa ggtatcgact tcaaagaaga
      781 cggtaacatc ctgggtcaca aactggaata caacttcaac tctcacaacg tttacatcac
      841 cgctgacaaa cagaaaaacg gtatcaaagc taacttcaaa atccgtcaca acgttgaaga
      901 cggttctgtt cagctggctg accactacca gcagaacacc ccgatcggtg acggtccggt
      961 tctgctgccg gacaaccact acctgtctac ccagtctgtt ctgtctaaag acccgaacga
     1021 aaaacgtgac cacatggttc tgctggaatt cgttaccgct gctggtatca cccacggtat
     1081 ggacgaactg tacaaagatg catgccagtt ctaacataac cctaatgagt gagctaactt
     1141 acattaattg cgttgcgcct taattaacgg cactcctcag caaatataat gaccctcttg
     1201 ataacccaag agggcatttt ttaatgccca tggcgtttac cacagctaa
//
