LOCUS       sequence-31747-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     Slug plasmid
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPGS-hSLUG.fl.flag
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    27..579
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    29..595
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(189..208)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     418..435
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     605..624
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        605..623
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     648..664
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     649..665
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(753..776)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctgcgtgcat ccatcttgtt caaccatatt atcatcgtgt ttttcaaagg aaaaccacgt
       61 ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg
      121 tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc
      181 ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa
      241 cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc
      301 agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg
      361 tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca
      421 ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt
      481 tccgggccct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
      541 cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg gcggaattcg
      601 cgcgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg
      661 tatcgataag cttgatatcg aattcctgca gcccggggga tccgcccggg ctagagcggc
      721 cgcgaattca ctagtgatta tcctcaaaca accttgtcat cgtcgtcctt gtagtcgtgt
      781 gctacacagc agccagattc ctcatgtttg tgcag
//
