LOCUS       sequence-317475-        1215 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of green fluorescent protein encoding zero 
            stop codons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: pJH805_pJH_LldR_Pcon-sfGFP.0TAG
FEATURES             Location/Qualifiers
     source          1..1215
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      20..47
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             349..1062
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
       61 actctgctag caagtaaggc cgaccaacaa cctaagggca attctctgat gaggattgcc
      121 cttttcttta ccagacatct ccccccacaa gcttcgctta tctgacctct ggttcacaat
      181 ttcccaatta aaactcacat caatgttgcc aatacataac atttagttaa ccattcattg
      241 tcattatccc tacacaacac ttacacaacg tgtgacaagg agatgagcaa cagactcatt
      301 acacgatgtg cgtggactcc catttaactt taagaaggag atatacatat gcgtaaaggt
      361 gaagaactgt tcaccggtgt tgttccgatc ctggttgaac tggacggtga cgttaacggt
      421 cacaaattct ctgttcgtgg tgaaggtgaa ggtgacgcta ccaacggtaa actgaccctg
      481 aaattcatct gcaccaccgg taaactgccg gttccgtggc cgaccctggt taccaccctg
      541 acctacggtg ttcagtgctt cgctcgttac ccggaccaca tgaaacagca cgacttcttc
      601 aaatctgcta tgccggaagg ttacgttcag gaacgtacca tctctttcaa agacgacggt
      661 acctacaaaa cccgtgctga agttaaattc gaaggtgaca ccctggttaa ccgtatcgaa
      721 ctgaaaggta tcgacttcaa agaagacggt aacatcctgg gtcacaaact ggaatacaac
      781 ttcaactctc acaacgttta catcaccgct gacaaacaga aaaacggtat caaagctaac
      841 ttcaaaatcc gtcacaacgt tgaagacggt tctgttcagc tggctgacca ctaccagcag
      901 aacaccccga tcggtgacgg tccggttctg ctgccggaca accactacct gtctacccag
      961 tctgttctgt ctaaagaccc gaacgaaaaa cgtgaccaca tggttctgct ggaattcgtt
     1021 accgctgctg gtatcaccca cggtatggac gaactgtaca aagatgcatg ccagttctaa
     1081 cataacccta atgagtgagc taacttacat taattgcgtt gcgccttaat taacggcact
     1141 cctcagcaaa tataatgacc ctcttgataa cccaagaggg cattttttaa tgcccatggc
     1201 gtttaccaca gctaa
//
