LOCUS       sequence-317477-        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of green fluorescent protein encoding two 
            stop codons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: pJH807_pJH_LldR_Pcon-sfGFP.2TAG
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      38..65
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc
       61 ctttttgcgt ttctacaaac tctgctagca agtaaggccg accaacaacc taagggcaat
      121 tctctgatga ggattgccct tttctttacc agacatctcc ccccacaagc ttcgcttatc
      181 tgacctctgg ttcacaattt cccaattaaa actcacatca atgttgccaa tacataacat
      241 ttagttaacc attcattgtc attatcccta cacaacactt acacaacgtg tgacaaggag
      301 atgagcaaca gactcattac acgatgtgcg tggactccca tttaacttta agaaggagat
      361 atacatatgc gtaaaggtga agaactgttc accggtgttg ttccgatcct ggttgaactg
      421 gacggtgacg ttaacggtca caaattctct gttcgtggtg aaggtgaagg tgacgctacc
      481 tagggtaaac tgaccctgaa attcatctgc accaccggta aactgccggt tccgtggccg
      541 accctggtta ccaccctgac ctacggtgtt cagtgcttcg ctcgttaccc ggaccacatg
      601 aaacagcacg acttcttcaa atctgctatg ccggaaggtt acgttcagga acgtaccatc
      661 tctttcaaag acgacggtac ctacaaaacc cgtgctgaag ttaaattcga aggtgacacc
      721 ctggttaacc gtatcgaact gaaaggtatc gacttcaaag aagacggtta gatcctgggt
      781 cacaaactgg aatacaactt caactctcac aacgtttaca tcaccgctga caaacagaaa
      841 aacggtatca aagctaactt caaaatccgt cacaacgttg aagacggttc tgttcagctg
      901 gctgaccact accagcagaa caccccgatc ggtgacggtc cggttctgct gccggacaac
      961 cactacctgt ctacccagtc tgttctgtct aaagacccga acgaaaaacg tgaccacatg
     1021 gttctgctgg aattcgttac cgctgctggt atcacccacg gtatggacga actgtacaaa
     1081 gatgcatgcc agttctaa
//
