LOCUS       sequence-31748-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Bommer GT, Feng Y, Iura A, Giordano TJ, Kuick R, Kadikoy H, Sikorski
            D, Wu R, Cho KR, Fearon ER
  TITLE     IRS1 regulation by Wnt/beta-catenin signaling and varied 
            contribution of IRS1 to the neoplastic phenotype.
  JOURNAL   J Biol Chem. 2010 Jan 15. 285(3):1928-38.
  PUBMED    19843521
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jan 15. 285(3):1928-38."
COMMENT     Sequence Label: IRS1-A4
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     42..58
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    126..144
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(190..209)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        355..854
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(390..413)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     787..806
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(879..898)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatcgatctc tcgaggtcga cggtatcgat
       61 aagcttaaaa agagcatgca caaacgcttc ttgacaggaa gagaagcatt tgtacatgct
      121 cgggatctct atcactgata gggaacttat aagattccca aatccaaaga catttcacgt
      181 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg
      241 tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca
      301 ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc taccgggtag
      361 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      421 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      481 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      541 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      601 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      661 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
      721 ccgggggcgg gctcaggggc gggctcangg gcggggcggg cgcccgaagg tcctccggag
      781 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
      841 ctccgggcct ttcgacctgc agcccaagct agcttaccat gaccgagtac aagcccacgg
      901 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcg
//
